The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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A Study on Contents of Vitamin K1 in Local Agricultural Products

지역농산물의 비타민 K1 함량 조사

  • Lee, A Reum (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Kim, Joo Hee (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Park, Jae-Ho (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Kim, Youngho (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Hong, Eui Yon (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Kim, Haeng-Ran (Dept. of Agro-Food Resources, NAAS, RDA) ;
  • Choi, Youngmin (Dept. of Agro-Food Resources, NAAS, RDA) ;
  • Lee, Junsoo (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Eom, Hyun-Ju (Chungcheongbukdo Agricultural Research and Extension Services)
  • Received : 2016.05.06
  • Accepted : 2016.05.27
  • Published : 2016.06.30
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Abstract

Vitamin K (phylloquinone) is an essential cofactor in the synthesis of active blood-clotting factors II, VII, IX and X. Deficiency of vitamin K leads to inadequate activity of these factors, resulting in bleeding. In this study, we investigated vitamin $K_1$ content of agricultural products that are widely and specifically grown in Korea including 9 leaves and vegetables, 16 fruits, and 11 cereals and specialty crops. Vitamin $K_1$ analysis of the agro-samples was by a validated, modified, reversed phase-HPLC method with fluorescence detection after post-column derivatization. The vitamin $K_1$ content ranged from 1.83 to $682.73{\mu}g$/100 g in leaves and vegetables, 0.17 to $28.22{\mu}g$/100 g in fruits, and ND to $279{\mu}g$/100 g in cereals and specialty crops. Among the 36 samples, high content of vitamin $K_1$ were found in Gugija (Lycium chinense Miller) leaves (average $682.73{\mu}g$/100 g) and Hansan ramie leaves (average $423.12{\mu}g$/100 g); however, mushroom, amaranth and Chinese artichoke showed no detectable levels. The results of ourstudy provide reliable vitamin $K_1$ content of Korean grown agricultural products that expand nutritional information and food composition database.

본 연구는 국내에서 많이 재배되는 농산물과 특수하게 재배되는 농산식품 자원(잎 및 채소류 9종, 과일류 16종, 곡류 및 특용작물 11종)을 선정하여 비타민 $K_1$의 함량 분석하여 기초데이터를 마련하고, 실험에 사용한 분석법을 검증하여 결과의 신뢰도를 확보하고자 하였다. 각 식품군의 비타민 $K_1$은 용매추출법을 이용하여 추출하였고, 형광 검출기를 이용하여 HPLC를 통해 정량하였다. 잎채소류 내 비타민 $K_1$의 범위는 $107.03{\sim}682.73{\mu}g$/100 g였으며, 구기자 잎에서 $682.73{\mu}g$/100 g으로 가장 높게 검출되었고, 대왕토란과 고구마는 각각 $1.83{\mu}g$/100 g과 $2.72{\sim}2.74{\mu}g$/100 g으로 낮은 함량을 보였다. 과일류의 비타민 $K_1$ 함량 범위는 $0.17{\sim}28.22{\mu}g$/100 g으로 분석되었고, 마른 반시에서 $28.22{\mu}g$/100 g으로 가장 높은 수치를 보였으며, 청포도 $14.97{\mu}g$/100 g, 키위류 $13.54{\sim}16.36{\mu}g$/100 g으로 다른 과일들과 비교해서 비교적 높은 함량을 보였다. 곡류의 비타민 $K_1$ 함량 범위는 $1.14{\sim}3.47{\mu}g$/100 g이었고, 느타리버섯, 이슬송이버섯, 초석잠에서는 모두 비타민 $K_1$이 검출되지 않았다. 검량선의 상관계수는 0.999로 높은 유의 수준을 보여 분석에 적합함을 알 수 있었으며, 반복성과 재현성의 변동계수 값은 각각 8.95%, 5.28%로 우수하였다. 따라서, 본 연구에서 분석된 자료는 국내에서 재배되는 지역농산물의 비타민 $K_1$ 기초데이터로 이용될 수 있을 것으로 판단된다.

Keywords

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