Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

A New Analysis of p-Coumaric Acid and Trans-cinnamic Acid in Propolis By UPLC

UPLC를 이용한 프로폴리스 중 p-Coumaric Acid와 Trans-cinnamic Acid 분석법

  • Kim, Se Gun (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hong, In Pyo (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Woo, Soon Ok (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Jang, Hye Ri (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Han, Sang Mi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 홍인표 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 우순옥 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 장혜리 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 한상미 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2016.02.13
  • Accepted : 2016.04.19
  • Published : 2016.06.30
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Abstract

A new UPLC-DAD method for simultaneous determination of p-coumaric acid and trans-cinnamic acid in propolis was developed using Halo-C18 column and step gradient elution of MeCN and $0.1%H_3PO_4$ in 8 min. The method was validated by specificity, linearity, limit of detection, limit of quantification, precision(intra- and inter-day variability) and recovery tests. The validated method was sufficiently applicable for quantitative analysis of propolis products as well as propolis collected from different regions in Korea. The established method is simple, appropriate and efficient, and can be used for quality assessment of Korean propolis.

Keywords

References

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