Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-Inflammatory and Antioxidant Effects of Clam Worm Extract in Macrophage RAW264.7 Cells

갯지렁이와 지렁이 추출물의 항염증 및 항산화 효과 비교

  • Kim, Se-woong (Department of Dental Pharmacology, School of Dentistry, Chonbuk National University) ;
  • Sapkota, Mahesh (Department of Dental Pharmacology, School of Dentistry, Chonbuk National University) ;
  • Li, Liang (Department of Dental Pharmacology, School of Dentistry, Chonbuk National University) ;
  • Yang, Ming (Department of Dental Pharmacology, School of Dentistry, Chonbuk National University) ;
  • Park, Chan-il (Institue of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Soh, Yunjo (Department of Dental Pharmacology, School of Dentistry, Chonbuk National University)
  • 김세웅 (전북대학교 치의학전문대학원 치과약리학교실) ;
  • 삽코타마헤쉬 (전북대학교 치의학전문대학원 치과약리학교실) ;
  • 이양 (전북대학교 치의학전문대학원 치과약리학교실) ;
  • 양명 (전북대학교 치의학전문대학원 치과약리학교실) ;
  • 박찬일 (경상대학교 해양과학대학) ;
  • 소윤조 (전북대학교 치의학전문대학원 치과약리학교실)
  • Received : 2016.04.04
  • Accepted : 2016.04.20
  • Published : 2016.06.30
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Abstract

Earth worm (Eisenia andrei) and clam worm (Perinereis linea) have been used as anti-bacterial and anti-inflammatory agent. However, it is unclear how they exerted their physiological effects in macrophages. In this experiment, the anti-inflammatory and antioxidant effects of clam worm extract (CWE) and earth worm extract (EWE) in RAW264.7 cells were examined by measuring MDA, catalase, SOD, GSH-Px and inflammatory cytokines (nitric oxide, iNOS, interleukin-$1{\beta}$ and tumor necrosis factor-${\alpha}$). Treatment with CWE significantly increased the activities of catalase, SOD and GSH-Px in RAW264.7 cells and decreased the level of MDA. Interestingly, treatment with CWE induced more activities of SOD than EWE. In addition, CWE decreased NO production, iNOS, COX-2, TNF-${\alpha}$ and IL-$1{\beta}$ in RAW264.7. The EWE also decreased NO production and iNOS, but increased COX-2 and IL-$1{\beta}$ suggesting that CWE could be better resources for anti-inflammatory and antioxidant agent than EWE. Taken together, these results indicate that CWE has the potential as a natural antioxidant and a therapeutic for inflammation-related diseases.

Keywords

References

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