Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Potentiating Activity of (+)-Usnic Acid on EDTA and Sodium Azide Methicillin-resistant Staphylococcus aureus

메티실린-내성 포도상구균에 대하여 EDTA 및 Sodium Azide 병용에 의한 우스닌산 약효증대

  • Lee, Young-Seob (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Hye-Sung (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University) ;
  • Lee, Jae Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Dae-Young (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Geum-Soog (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Hyoun-Wook (Animal Products Utilization Division, National Institute of Animal Science, RDA) ;
  • Noh, Geon-Min (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Lee, Seung Eun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Sun Ae (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University) ;
  • Song, Ok Hee (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University)
  • 이영섭 (농촌진흥청 국립원예특작과학원 인삼특작이용팀) ;
  • 김혜성 (원광대학교 약학대학 한약학과) ;
  • 이재원 (농촌진흥청 국립원예특작과학원 인삼특작이용팀) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작이용팀) ;
  • 김금숙 (농촌진흥청 국립원예특작과학원 인삼특작이용팀) ;
  • 김현욱 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 노건민 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작이용팀) ;
  • 이순애 (원광대학교 약학대학 한약학과) ;
  • 송옥희 (원광대학교 약학대학 한약학과) ;
  • 권동렬 (원광대학교 약학대학 한약학과)
  • Received : 2016.04.29
  • Accepted : 2016.06.16
  • Published : 2016.06.30
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Abstract

Methicillin-Resistant Staphylococcus aureus(MRSA) is a multidrug-resistant(MDR) strain. (+)-Usnic acid(UA) is uniquely found in lichens, and is especially abundant in genera such as Usnea and Cladonia. UA has antimicrobial activity against human and plant pathogens. Therefore, UA may be a good antibacterial drug candidate for clinical development. In search of a natural products capable of inhibiting this multidrug-resistant bacteria, we have investigated the antimicrobial activity of UA against 17 different strains of the bacterium. In this study, the effects of a combination of UA and permeable agents against MRSA were investigated. For the measurement of cell wall permeability, UA with concentration of Ethylenediaminetetraacetic acid(EDTA) was used. In the other hand, Sodium azide($NaN_3$) was used as inhibitors of ATPase. Against the 17 strains, the minimum inhibitory concentrations(MICs) of UA were in the range of $7.81-31.25{\mu}g/ml$. EDTA or $NaN_3$ cooperation against MRSA showed synergistic activity on cell wall. UA and in combination with EDTA and $NaN_3$ could lead to the development of new combination antibiotics against MRSA infection.

Keywords

References

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