Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Diels-Alder type adducts from the fruits of Morus alba L.

오디(Morus alba fruit)로 부터 Diels-Alder형 부가체 화합물의 분리 및 동정

  • Lee, Yeong-Geun (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Seo, Kyeong-Hwa (Natural Medicine Research Center, KRIBB) ;
  • Hong, Eock-Kee (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Dong-Man (Korea Food Research Institute) ;
  • Kim, Young-Eon (Korea Food Research Institute) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University)
  • Received : 2015.11.30
  • Accepted : 2016.01.28
  • Published : 2016.06.30
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Abstract

The fruits of Morus alba L. were extracted with 80 % aqueous MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated silica gel ($SiO_2$) and octadecyl silica gel column chromatographies for the EtOAc and n-butyl alcohol fractions led to isolation of two phenolic compounds. The chemical structures of the compounds were determined as Diels-Alder type adducts, mulberrofuran E (1) and chalcomoracin (2) based on spectroscopic data analyses including nuclear magnetic resonance, mass spectrometry, and infrared spectrometry. Compounds 1 and 2 were isolated for the first time from the fruits of M. alba L. in this study.

오디(Morus alba L.) 를 실온에서 80 % MeOH 수용액으로 추출하고 이 추출물을 EtOAc, n-butyl alcohol, 그리고 물 분획으로 나누었다. EtOAc 분획에 대하여 $SiO_2$ 및 ODS column chromatography를 반복 실시하여 2종의 Diels-Alder 부가화합물을 분리, 정제하였다. NMR, IR 및 ESI/MS data를 해석하여, 화합물 1과 2를 mulberrofuran E와 chalcomoracin 으로 각각 구조동정 하였다. 두 화합물 모두 오디로부터는 이번 실험에서 처음으로 분리되었다.

Keywords

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