DOI QR코드

DOI QR Code

Structural Characterization of pre-miRNA 155

  • Kim, Won-Je (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Shin, JiYeon (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Bang, Kyeongmi (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Song, Hyun Kyu (Division of Life Sciences, Korea University) ;
  • Kim, Nak-Kyoon (Advanced Analysis Center, Korea Institute of Science and Technology)
  • Received : 2016.03.10
  • Accepted : 2016.05.02
  • Published : 2016.06.20

Abstract

MiRNA-155, upregulated in various cancers, is one of the miRNAs that suppress apoptosis of human cancer. Thus, inhibition of the maturation of miRNA-155 could be an effective way to induce apoptotic cancer cell death. The apical stem-loop of the pre-miRNA-155 has been known as a Dicer biding site for RNA cleavage. Here, to understand the molecular basis of the tertiary interaction between pre-miRNA-155 with Dicer, we characterize the structure of the apical stem-loop of pre-miRNA-155 using NMR spectroscopy. The RNA has a stem-bulge-stem-loop-stem structure, which is consist of G-C Watson-Crick and G-U Wobble base pairs. The assignments of imino- protons were further confirmed by 2D $^{15}N-^1H$ HSQC NMR spectrum. The NMR parameters obtained in this study can be further used to investigate the tertiary interaction between pre-miRNA-155 and other biomolecules such as protein, nucleic acids, or small chemicals which might be used to control the apoptosis of cancer.

Keywords

References

  1. M. Ha and V. N. Kim, Nat. Rev. Mol. Cell Biol. 15, 509 (2014) https://doi.org/10.1038/nrm3838
  2. A. Esquela-Kerscher and F. J. Slack, Nat. Rev. Cancer 6, 259 (2006) https://doi.org/10.1038/nrc1840
  3. J. Pai, S. Hyun, J. Y. Hyun, S. H. Park, W.-J. Kim, S. H. Bae, N-.K. Kim, J. Yu, and I. Shin, J. Am. Chem. Soc., 138, 857 (2016) https://doi.org/10.1021/jacs.5b09216
  4. N-.K.Kim, Y-.S.Nam, and K-.B Lee, J. Kor. Mag. Reson. Soc. 18, 5 (2014) https://doi.org/10.6564/JKMRS.2014.18.1.005
  5. W-.J. Lee and N-.K.Kim, J. Kor. Mag. Reson. Soc. 19, 143 (2015) https://doi.org/10.6564/JKMRS.2015.19.3.143
  6. J. Gullerez,, P.J. Lopez,, F. Proux,, H. Launay, and M. Dreyfus, Proc. Natl. Acad. Sci. U.S.A. 102, 5958 (2005) https://doi.org/10.1073/pnas.0407141102
  7. M. Zuker, Nucleic Acids Res. 31, 3406 (2003) https://doi.org/10.1093/nar/gkg595
  8. A. J. Dingley and S. Grzesiek, J. Am. Chem. Soc. 120, 8293 (1998) https://doi.org/10.1021/ja981513x