Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Antioxidant Activity of Solvent Fractions from Distylium racemosum in Jeju

제주 자생 조록나무 분획물의 항산화 효과

  • Kim, Hye-Ran (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Park, Gyu-Nam (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Jung, Bo-Kyoung (Department of Clinical Laboratory Science, Catholic University of Pusan) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Jung, Yong-Hwan (Jeju Biodiversity Research Institute, Jeju Technopark) ;
  • Chang, Kyung-Soo (Department of Clinical Laboratory Science, Catholic University of Pusan)
  • 김혜란 (부산가톨릭대학교 임상병리학과) ;
  • 박규남 (부산가톨릭대학교 임상병리학과) ;
  • 정보경 (부산가톨릭대학교 임상병리학과) ;
  • 윤원종 (제주테크노파크 생물종다양성연구소) ;
  • 정용환 (제주테크노파크 생물종다양성연구소) ;
  • 장경수 (부산가톨릭대학교 임상병리학과)
  • Received : 2016.02.23
  • Accepted : 2016.03.07
  • Published : 2016.06.30
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Abstract

Anti-oxidant activity of 600 medicinal plants from Jeju was analyzed. Extracts from the leaves of Distylium racemosum have the highest anti-oxidant activity. D. racemosum is an oriental medicinal plant belonging to the Hamamelidacea and grows in the wild in Jeju. This study was conducted to evaluate the antioxidant and cell viability of different fractions (n-hexane, methylene chloride, ethyl acetate, buthanol, DW) from D. racemosum. Anti-oxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test and total phenolic content. Cell viability was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) cell viability assay on HepG2 and A549 cells. Among various extracts, the ethyl acetate fraction showed the highest DPPH radical scavenging activity, reaching approximately 93% at 0.5 mg/mL, higher than that of quercetin used as a positive control. Ethyl acetate fractions showed the highest total phenolic content at 505 mg GAE/g. The phenolic content of each extract showed association with DPPH radical scavenging activity. The ethyl acetate extracts were resistant against hydrogen peroxide ($H_2O_2$) treatment in the MTT cell viability assay and showed a higher cell protective effect than other fraction extracts. These results suggest that the ethyl acetate fraction might be a source of anti-oxidants of D. racemosum.

600여 종의 제주 천연 추출물을 이용하여 항산화 효과를 스크린한 결과, 조록나무 잎 추출물이 가장 높은 항산화 효과를 나타내었다. 조록나무는 조록나무과에 속하는 상록 나무로써 제주 천연자원이다. 본 연구에서는 극성이 다른 유기용매(헥산, 메틸렌 클로라이드, 에틸 아세테이트, 부탄올, 물)로 순차적으로 분획 한 조록나무 물질 5가지를 이용하여 항산화 효과 및 간세포와 폐세포를 이용하여 세포 보호능을 확인하였다. 에틸 아세테이트 분획물질은 0.5 mg/mL에서 약93%로 가장 우수한 DPPH 라디칼 소거능을 보이며 양성 대조군으로 사용한 quercetin 보다 높은 효능을 보였다. 총 페놀 함량을 확인 한 결과, 에틸 아세테이트 분획물질은 505 mg GAE/g로 가장 높은 총 페놀 함량을 보였으며, 모든 추출물에서 DPPH 라디칼 소거능과 연관성 있는 결과를 보였다. 또한 에틸 아세테이트 분획물질은 $H_2O_2$에 대한 산화적 스트레스로부터 다른 분획 물질보다 높은 세포보호효과를 나타내었다. 본 연구를 통해 조록나무의 에틸 아세테이트 분획물질의 높은 항산화 효과를 확인하였으며, 천연 항산화 소재의 가능성이 높을 것으로 사료된다.

Keywords

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