The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects on Anti-inflammatory Action in HaCaT Cells and Inhibiting Sebum Secretion in SEB-1 Cells by Gleditsiae Fructus Extract

조협 추출물이 HaCaT cells의 항염증과 SEB-1 cells의 피지분비 억제에 미치는 영향

  • Koo, Eun Jin (Department of Pediatrics, College of Korean Medicine, Daejeon University) ;
  • Han, Jae Kyung (Department of Pediatrics, College of Korean Medicine, Daejeon University) ;
  • Kim, Yun Hee (Department of Pediatrics, College of Korean Medicine, Daejeon University)
  • 구은진 (대전대학교 한의과대학 소아과학교실) ;
  • 한재경 (대전대학교 한의과대학 소아과학교실) ;
  • 김윤희 (대전대학교 한의과대학 소아과학교실)
  • Received : 2016.04.22
  • Accepted : 2016.05.16
  • Published : 2016.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives The purpose of this study is to investigate the effects of Gleditsiae Fructus 70% EtOH extract (JS_E) on anti-inflammatory action in HaCaT cells (A spontaneously immortalized human keratinocyte cell line) and inhibiting triglyceride genesis in SEB-1 cells (Immortalized human sebocyte). Methods The anti-inflammatory effect of JS_E was analyzed by enzyme-linked immunosorbent assays (ELISA) which measured levels of IP-10, RANTES and MDC in HaCaT cells. Also the effect on secretion of sebum of JS_E was analyzed by TG-S kit which measured the quantity of triglyceride in SEB-1 cells. Results JS_E inhibited IP-10, RANTES and MDC expression in a dose dependent manner. IP-10 expression was inhibited significantly in comparison to TNF-${\alpha}$ and IFN-${\gamma}$ recombination (TI) control group at concentration of JS_E $200{\mu}g/ml$ and RANTES and MDC expressions were inhibited significantly at concentration of JS_E 100, $200{\mu}g/ml$. JS_E also inhibited triglyceride secretion of SEB-1 cells significantly in comparison to the control group in a dose dependent manner. Conclusions This study shows that JS_E has the effects of anti-inflammatory action and inhibiting sebum secretion. According to these results, JS_E can be used for treating skin diseases such as acne and dermatitis caused by inflammation and excessive secretion of sebum by controlling the activity of the HaCaT and SEB-1 cells.

Keywords

References

  1. Jung JY. ACNE. Seoul: MDworld Co. 2007:15-35.
  2. Hwang KY. ABC of dermatology. Seoul: Medbook Co. 2001:91-9.
  3. Yu M. Review of the kind, pathogenesis, treatment and management of acne. Grad School Konkuk Univ. 2003.
  4. Kim KB, Kim DG, Kim YH, Kim JH, Min SY, Park EJ, Baek JH, Sung HK, Yu SA, Lee SY, Lee JY, Chang GT, Jung MJ, Chai JW, Chun JH, Han YJ, Han JK. Pediatr Korean Medicine. Seoul: EuiSeongDang Publishing Co. 2015:398-401.
  5. Roh SS. Wonsaek Dermatology. Seoul: Ibicykihuik Co. 2006:737-41.
  6. The society of Korean medicine obstetrics and gynecology. Hanbangyeosunguihak. Seoul: EuiSeongDang Publishing Co. 2012:866-75.
  7. Kim KL. Antimicrobial activity and acne improvement effect of ethanol extract from Caesalpinia Sappan. Grad School Wonkwang Univ. 2009.
  8. Lee SY. The effects of Sulfur extract on anti-inflammation and anti-propionibacterium acne. Grad School Sangji Univ. 2007.
  9. Yoon JY. Effects of Epigallocatechin-3-gallate on P. acnes and sebocytes. Grad School Konkuk Univ. 2011.
  10. Jeon OD. The effects of Houttuyniae herba extract on anti-inflammation and anti-propionibacterium acnes. Grad School Sangji Univ. 2007.
  11. Kim NK, Kim MH, Yoon CS, Choi SW. Studies on the anti-inflammatory activity of Paulownia Coreana Uyeki leaf extract. J Soc Cosmet Sci Korea. 2006;32(4):241-7.
  12. Tak MJ. The inhibitory effects of Yang Geouk San Hwa Tang on LPS-stimulated inflammation in RAW264.7 macrophage cells. Grad School Dongeui Univ. 2010.
  13. Choi KH. The effects of Jeondo-San on anti-inflammation and anti-propionibacterium acnes. Grad School Sangji Univ. 2007.
  14. Hong SH, Roh SS. The study on the effect of Chungsang BangpungTang and Lonicerae flos, Coicis semen (CBTLC) on acnes. J Korean Orient Med Ophthalmol Otolaryngol Dermatol. 2002;15(1):315-35.
  15. Hyung YK. The study on the effect of Okyongsan in acnes. Grad School Woosuk Univ. 1998.
  16. Yim HS. The experimental study of Gagambipachungpe-eum on acne. Grad School Kyunghee Univ. 2000.
  17. Kim SB. Effect of the addition temperament drugs of Yeongyopaedock-san on acne in the state of inflammation. Grad School Gachon Univ. 2002.
  18. Yoo JG. The effects of Yeouigeumhwang-san on anti-inflammation and anti-propionibacterium acnes. Grad School Sangji Univ. 2007.
  19. Lee YS, Yoon JY, Han JK, Kim YH. A case study about the effect of Yukmijihwangtang Gamibang and comedon extraction on male acne. Collection of dissertations. Research Institute of Korean Med, Daejeon Univ. 2011;20(1):119-25.
  20. Kim JH, Yoo DY. A case report of acne vulgaris patient treated with Cheongsangbangpung-tang. Collection of dissertations, Research Institute of Korean Med, Daejeon Univ. 2013;22(1):185-91.
  21. Kim MA, Kim MB, Shin SH, Byun SM, Ko WS, Lee HS, Park SK, Yoon HJ. The study on external treatment of oriental medical care on acne. J Korean Orient Med Ophtalmol Otolaryngol Dermatol. 2008;21(2):102-11.
  22. Hong SH. The clinical study on the effect of Jeondo-san (Diandao-san) on acnes. J Korean Med. 2005;26(3):74-9.
  23. Hanuigwadaehak Bonchohak Pyeonchanwiwonhoe. Bonchohak. Seoul: Yeongrimsa Publishing Co. 2008:490-1.
  24. Shin KG. Shincibonchohak. Seoul: Sumunsa Publishing Co. 1973:404-7.
  25. Jang KA. Hankulkyoungakjeonsu (2). Seoul: Hanmiuihak Publishing Co. 2011:58.
  26. Lee SJ. Bonchogangmok. Seoul: Komunsa Publishing Co. 1993:1153-7.
  27. Heo J. Shindaeyukdonguibokam. Seoul: Bubinmunhwasa Publishing Co. 2009:597-9.
  28. Lee EO, Yu CS. Prosapogenin in pods of Gleditschia officinalis. J Korean Pharmacog. 1978;9(2):93-7.
  29. Seo BI. A philological study on poisoning of Glenditsiae fructus. J East West Med. 2008;33(1):15-28.
  30. Kim YJ, Kim J, Park HJ, Lee CM. Inhibition effect of Gleditsia sinensis lam on T tyrosinase activity and reactive oxygen species related to Melanin biosynthes. J Korean Biotechnol Bioengineering. 2005;15:541-3.
  31. Park DY. Antioxidant activities of extracts from Gleditsiae fructus. Grad School Chungang Univ. 2012.
  32. Berridge MV, Tan AS. Characterisation of the cellular reduction of 3-(4,5-dimethylthiazol-2yl)-2,5-dipheny ltetrazolium bromide (MTT): Subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Archives Biochem Biophys. 1993;303:474-82. https://doi.org/10.1006/abbi.1993.1311
  33. Park HJ, Kim MM, Lee DH. Effect of saponin with antioxidant activity on matrix Metalloproteinase in human dermal fibroblasts. Kor J Life Sci. 2011;21(9):1266-73. https://doi.org/10.5352/JLS.2011.21.9.1266
  34. Joh EH. Anti-inflammatory effects of saponins derived from natural products. Grad School KyungHee Univ. 2015.
  35. Geum JH. Compounds from the spina of Gleditsia japonica and their anti-inflammatory. Grad School Catholic Univ of Daegu. 2015.
  36. Seo CS, Lim HS, Ha H, Jin SE, Shin HK. Quantitative analysis and anti-inflammatory effects of Gleditsia sinensis thorns in RAW 264.7 macrophages and HaCaT keratinocytes. Mol Med Rep. 2015;12(3):4773-81. https://doi.org/10.3892/mmr.2015.3936
  37. Park EH, Shin MJ. Anti-inflammator activity of aqueous extrat from Gleditsiae spina. J Korean Pharm Soci. 1993;37(2):124-8
  38. You JY. The anti-inflammatory activity of ethanol extracts of Gleditsia japonica Miquel var. Koraiensis Nakai in raw 264.7 cells. Grad school Chung-ang Univ. 2009.
  39. Park NH. Anti-inflammatory effects of GST extract fermented by lactic acid bacteria on mouse macrophage cells and the CIA mouse model. Grad School Korea Univ. 2015.
  40. Han YS, Choi TB, Lee YH, Kim CJ, Kim JY, Jang MH. Dermatology (3rd edition). Seoul: Jungdammedia. 2011:18-22, 75-80.
  41. Lee KE, Nam JJ, Kim SM, Kim HK, Moon SJ, Youm JK. Anti-inflammatory effects of the mixture of Sorbus commixta, Urtica dioica, Phyllostachys nigra, and Rhus semialata Gall extracts on LPS-induced inflammation in HaCaT cells. J Soc Cosmet Sci Korea. 2014;40(1):45-54 https://doi.org/10.15230/SCSK.2014.40.1.45
  42. Kim HJ, Kim WS, Park HJ, Moon G, Kim DW, Won JH, Kim YK. Inhibitory effect of Jaeumganghwa-tang on allergic inflammatory reaction. Korean J Orient Int Med. 2004;25(2):174-82.
  43. Andrew D Luster. The role of chemokines in linking innate and adaptive immunity. Curr Opin Immunol. 2002;14(1):129-35. https://doi.org/10.1016/S0952-7915(01)00308-9
  44. Park SJ, Kim YM, Lim KS, Woo WH, Mun YJ. Inhibitory effect of extract of Ficis folium on the Sebum synthesis in human sebocyte cell line (SZ95). J Korean Orient Physiol Pathol. 2006;20(4):919-23.
  45. Monica O, Emanuela C, Mauro P. Lipid mediators in Acne. J Mediat Inflamm. 2010:Article ID 858176.
  46. Park SM. Studies on the effects of sucrose disinapate isolated from Raphanus sativus for the production of sebum in sebocytes. Grad School Hanbat Univ. 2014.
  47. Yoon JY. Effects of Epigallocatechin-3-gallate on P.acnes and sebocytes. Grad School Konkuk Univ. 2011.
  48. Park JS. Evaluation of the sebosuppressive activity of topical Glycyrrhizinitic acid. Grad School Korea Univ. 2004.
  49. Higaki. Lipase inhibitors for the treatment of acne. J Mol Catal B Enzym. 2003;22(5/6):377-84. https://doi.org/10.1016/S1381-1177(03)00053-5

Cited by

  1. 국내 한약 치험례 분석을 통한 한의학적 여드름 치료법에 대한 고찰 vol.31, pp.4, 2018, https://doi.org/10.6114/jkood.2018.31.4.065
  2. 모돈 군사 사육의 깔짚과 슬랏바닥의 행동 및 환경 수준 평가 비교 vol.21, pp.8, 2016, https://doi.org/10.5762/kais.2020.21.8.388
  3. 조협 (皂莢, Gleditsiae Fructus) n-hexane 추출물이 NC/Nga Tnd mouse의 아토피 피부염에 미치는 영향 vol.35, pp.1, 2016, https://doi.org/10.7778/jpkm.2021.35.1.76