The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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The Anti-inflammatory Effects of Hataedock Taken Douchi Extracts on Atopic Dermatitis-like Skin Lesion of NC/Nga Mouse

두시(豆豉) 추출물을 이용한 하태독법(下胎毒法)이 NC/Nga 생쥐에서 유발된 아토피 유도 피부염에 미치는 항염증 효과

  • Aum, Sun Ho (Department of Pediatrics, School of Korean Medicine, Pusan National University) ;
  • Ahn, Sang Hyun (Research Institute for Korean Medicine, Pusan National University) ;
  • Park, Sun Young (Department of Physiology, College of Korean Medicine, Semyung University) ;
  • Cheon, Jin Hong (Department of Pediatrics, School of Korean Medicine, Pusan National University) ;
  • Kim, Ki Bong (Department of Pediatrics, School of Korean Medicine, Pusan National University)
  • 엄선호 (부산대학교 한의학전문대학원 소아과학교실) ;
  • 안상현 (부산대학교 한의과학연구소) ;
  • 박선영 (세명대학교 한의과대학 생리학교실) ;
  • 천진홍 (부산대학교 한의학전문대학원 소아과학교실) ;
  • 김기봉 (부산대학교 한의학전문대학원 소아과학교실)
  • Received : 2016.04.22
  • Accepted : 2016.05.16
  • Published : 2016.05.31
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Abstract

Objectives Hataedock is a Korean herbal medical oral treatment that removes fetal toxic heat and meconium from new born babies. The purpose of this study is to evaluate whether Hataedock treatment of Duchi extracts has anti-inflammation effects on AD (Atopic Dermatitis)-induced NC/Nga mice. Methods After Hataedock treatment of Duchi extracts on days 0, 3-week-old NC/Nga mice were sensitized on days 28, 35, 42 by exposure of DNFB (dinitrochlorobenzene) and were induced to have AD. Immunohistochemistry of NF-${\kappa}B$ p65, iNOS, COX-2 and TUNEL assay of apoptotic body was used to identify changes of skin damages and anti-inflammation effects. Results The alleviate effect of the skin damage and angiogenesis was observed in DT group. The damage of stratum corneum, hyperplasia, edema, infiltration of lymphocytes and distribution of capillary were decreased in DT group. Also, the study results suggested that Hataedock treatment of Duchi extracts in DT group remarkably downregulated levels of NF-${\kappa}B$ p65 by 70% (p < 0.001), as well as COX-2 by 51%, iNOS by 62% (p < 0.001). Additionally, Hataedock treatment of Duchi extracts in DT group up-regulated apoptosis of inflammatory cells by 68% in atopic dermatitis-like skin lesion. Conclusions From the study results, we observed that Hataedock treatment of Duchi extracts alleviates AD through diminishing various inflammatory cytokines in the skin lesions, which are involved in the initial steps of AD development. It is anticipated to have potential applications for prevention and treatment of atopic dermatitis.

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References

  1. Flohr C, Mann J. New insights into the epidemiology of childhood atopic dermatitis. Allergy. 2014;69(1):3-16. https://doi.org/10.1111/all.12270
  2. Suh SH. Prevalence of allergic diseases in Korean children, 2010. Health Dis. 2011;4:425-31.
  3. Schneider L, Tilles S, Lio P, Boguniewicz M, Beck L, LeBovidge J, Novak N. Atopic dermatitis: a practice parameter update 2012. J Allergy Clin Immunol. 2013;131(2):295-9. https://doi.org/10.1016/j.jaci.2012.12.672
  4. Kabashima K. New concept of the pathogenesis of atopic dermatitis: interplay among the barrier, allergy, and pruritus as a trinity. J Invest Dermatol. 2013;70:3-11.
  5. Manti S, Chimenz R, Salpietro A, Colavita L, Pennisi P, Pidone C, Sturiale M, Arrigo T, Miraglia Del Giudice M, Salpietro C, Cuppari C. Atopic dermatitis: expression of immunological imbalance. J Biol Regul Homeost Agents. 2015;29:13-7.
  6. Park YM. Advances in the pathophysiology of atopic dermatitis. Allergy Asthma Respir Dis. 2006;16(3):189-96.
  7. Ministry of Health and Welfare. Korea Health Statistics 2012: Korea National Health and Nutrition Examination Survey [KNHANES V-3].
  8. Halonen M, Lohman IC, Stern DA, Spangenberg A, Anderson D, Mobley S, Ciano K, Peck M, Wright AL. Th1/Th2 patterns and balance in cytokine production in the parents and infants of a large birth cohort. J Immunol. 2009;182(5):3285-93. https://doi.org/10.4049/jimmunol.0711996
  9. Im GM, Jeong HW, Kim HS, Jeong WY. Oriental medical approach on the allergic disease. Kor J Orient Physiol Pathol. 2002;16(5):831-9.
  10. Kang MY, Jang GT, Kim JH. A study on fetal toxicosis removal therapy. J Pediatr Korean Med. 2003;17(1):29-51.
  11. Hopkins JE, Naisbitt DJ, Kitteringham NR, Dearman RJ, Kimber I, Park BK. Selective haptenation of cellular or extracellular protein by chemical allergens: association with cytokine polarization. Chem Res Toxicol. 2005;18(2):375-81. https://doi.org/10.1021/tx049688+
  12. Park MC, Choi IH. The efficacy of Sihocheonggan-san (Chaihuqinggan-san) extract to improve the function of Stratum Corneum on mice model after atopic dermatitis elicitation. J Kor Orient Med. 2004;25(3):137-48.
  13. Baeuerle PA, Baltimore D. NF-kappa B: ten years after. Cell. 1996;87(1):13-20. https://doi.org/10.1016/S0092-8674(00)81318-5
  14. Bruch-Gerharz D, Fehsel K, Suschek C, Michel G, Ruzicka T, Kolb-Bachofen V. A proinflammatory activity of interleukin 8 in human skin: expression of the inducible nitric oxide synthase in psoriatic lesions and cultured keratinocytes. J Exp Med. 1996;184(5):2007-12. https://doi.org/10.1084/jem.184.5.2007
  15. Bruch-Gerharz D, Ruzicka T, Kolb-Bachofen V. Nitric oxide and its implications in skin homeostasis and disease - a review. Arch Dermatol Res. 1998;290(12):643-51. https://doi.org/10.1007/s004030050367
  16. Heo J. DongUiBoGam. 2nd ed. Seoul: NamSanDang. 2004.
  17. Yeh CY, Jung CJ, Huang CN, Huang YC, Lien HT, Wang WB, Wang LF, Chia JS. A legume product fermented by Saccharomyces cerevisiae modulates cutaneous atopic dermatitis-like inflammation in mice. BMC Complement Altern Med. 2014;14:194. https://doi.org/10.1186/1472-6882-14-194
  18. Matsuda A, Tanaka A, Pan W, Okamoto N, Oida K, Kingyo N, Amagai Y, Xia Y, Jang H, Nishikawa N, Kajiwara N, Ahn G, Ohmori K, Matsuda H. Supplementation of the fermented soy product immubalance effectively reduces itching behavior of atopic NC/Tnd mice. J Dermatol Sci. 2012;67(2):130-9. https://doi.org/10.1016/j.jdermsci.2012.05.011
  19. Zhang T, Pan W, Takebe M, Schofield B, Sampson H, Li XM. Therapeutic effects of a fermented soy product on peanut hypersensitivity is associated with modulation of T-helper type 1 and T-helper type 2 responses. Clin Exp Allergy. 2008;38(11):1808-18.
  20. Bao ZS, Hong L, Guan Y, Dong XW, Zheng HS, Tan GL, Xie QM. Inhibition of airway inflammation, hyperresponsiveness and remodeling by soy isoflavone in a murine model of allergic asthma. Int Immunopharmacol. 2011;11(8):899-906. https://doi.org/10.1016/j.intimp.2011.02.001
  21. Akiyama T, Ishida J, Nakagawa S, Ogawara H, Watanabe S, Itoh N, Shibuya M, Fukami Y. Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem. 1987;262(12):5592-5.
  22. Robert V, Triffaux E, Paulet PE, Guery JC, Pelletier L, Savignac M. Protein kinase C-dependent activation of CaV1.2 channels selectively controls human Th2-lymphocyte functions. J Allergy Clin Immunol. 2014;133(4):1175. https://doi.org/10.1016/j.jaci.2013.10.038
  23. Yang JQ, Leitges M, Duran A, Diaz-Meco MT, Moscat J. Loss of PKC lambda/iota impairs Th2 establishment and allergic airway inflammation in vivo. Proc Natl Acad Sci USA. 2009;106(4):1099-104. https://doi.org/10.1073/pnas.0805907106
  24. Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med. 2003;9(6):669-76. https://doi.org/10.1038/nm0603-669
  25. Jung YJ, Isaacs JS, Lee S, Trepel J, Neckers L. IL-1beta-mediated up-regulation of HIF-1alpha via an NFkappaB/COX-2 pathway identifies HIF-1 as a critical link between inflammation and oncogenesis. FASEB J. 2003;17(14):2115-7. https://doi.org/10.1096/fj.03-0329fje
  26. Kroncke KD, Fehsel K, Kolb-Bachofen V. Nitric oxide: cytotoxicity versus cytoprotection-how, why, when, and where?. Nitric Oxide. 1997;1(2):107-20. https://doi.org/10.1006/niox.1997.0118
  27. Dong Z, Saikumar P, Weinberg JM, Venkatachalam MA. Calcium in cell injury and death. Annu Rev Pathol. 2006;1:405-34. https://doi.org/10.1146/annurev.pathol.1.110304.100218