Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Extraction of Active Ingredient from Angelica Using Microwave Energy

마이크로웨이브 에너지를 이용한 안젤리카로부터 유효성분의 추출

  • Lee, Seung Bum (Department of Chemical Engineering, Dankook University) ;
  • Kim, Hoon Gi (Department of Chemical Engineering, Dankook University) ;
  • Jeon, Gil Song (Department of Chemical Engineering, Dankook University) ;
  • Hong, In Kwon (Department of Chemical Engineering, Dankook University)
  • Received : 2016.04.05
  • Accepted : 2016.05.15
  • Published : 2016.06.10
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Abstract

In this study, the extractable content of each parameters from angelica was confirmed using conventional solvent extraction and microwave extraction in order to extract the functional active ingredient. In addition, the functionality of active ingredients was evaluated by measuring the antioxidant activity and the flavonoids and total polyphenols of the active ingredient extracted from angelica, For the conventional solvent extraction at optimal conditions of the extraction time (2 h), ethanol/pure water volume ratio (60%), the extraction temperature ($60^{\circ}C$), 20.6 wt% of the active ingredient were extracted. Also, when using microwave extraction at optimal conditions of the microwave irradiation time (6 min), microwave intensity (600 W) and ethanol/pure water volume ratio (60 vol%) 22.8 wt% of the active ingredient were extracted. The microwave method required shorter time to complete extraction compared to that of using the conventional solvent extraction method. The antioxidant activity of active ingredients extracted from angelica was 31.46% of DPPH radical scavening activity. The flavonoid content was 14.20 mg QE/mg dw, and total polyphenol content was 11.70 mg GAE/g when using the microwave extraction process.

본 연구에서는 안젤리카로부터 기능성 유효성분을 추출하기 위해 기존의 용매추출법과 마이크로웨이브 추출법을 이용하여 각 변수에 따른 추출량을 확인하였다. 또한 안젤리카로부터 추출된 유효성분의 항산화능 및 플라보노이드성분 함량, 총폴리페놀성분 함량 등을 측정함으로써 유효성분의 기능성을 평가하였다. 기존의 용매추출법의 최적조건인 추출시간(2 h), 주정/초순수의 부피비(60 vol%), 추출온도($60^{\circ}C$)에서 20.6 wt%의 유효성분이 추출되었다. 또한 마이크로웨이브 추출법의 최적조건인 마이크로웨이브 조사시간(6 min), 마이크로웨이브 세기(600 W), 주정/초순수의 부피비(60 vol%)의 조건에서 유효성분이 22.8 wt% 추출되어 기존의 용매추출법에 비해 빠른 시간에 추출이 완료됨을 알 수 있었다. 안젤리카로부터 추출된 유효성분의 추출량 유효성분의 항산화능은 DPPH radical scavening activity로 31.46%이었고, 플라보노이드성분 함량은 14.20 mg QE/mg dw, 그리고 총 폴리페놀 함량은 11.70 mg GAE/g으로 나타났다.

Keywords

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