Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
권호 표지
DOI QR코드

DOI QR Code

Polypyrrole-incorporated conductive hyaluronic acid hydrogels

  • Yang, Jongcheol (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST)) ;
  • Choe, Goeun (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST)) ;
  • Yang, Sumi (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST)) ;
  • Jo, Hyerim (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST)) ;
  • Lee, Jae Young (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST))
  • Received : 2016.07.28
  • Accepted : 2016.09.14
  • Published : 2016.12.01
⟨ Previous Next ⟩

Abstract

Background: Hydrogels that possess hydrophilic and soft characteristics have been widely used in various biomedical applications, such as tissue engineering scaffolds and drug delivery. Conventional hydrogels are not electrically conductive and thus their electrical communication with biological systems is limited. Method: To create electrically conductive hydrogels, we fabricated composite hydrogels of hyaluronic acid and polypyrrole. In particular, we synthesized and used pyrrole-hyaluronic acid-conjugates and further chemically polymerized polypyrrole with the conjugates for the production of conductive hydrogels that can display suitable mechanical and structural properties. Results: Various characterization methods, using a rheometer, a scanning electron microscope, and an electrochemical analyzer, revealed that the PPy/HA hydrogels were soft and conductive with ~ 3 kPa Young's modulus and ~ 7.3 mS/cm conductivity. Our preliminary in vitro culture studies showed that fibroblasts were well attached and grew on the conductive hydrogels. Conclusion: These new conductive hydrogels will be greatly beneficial in fields of biomaterials in which electrical properties are important such as tissue engineering scaffolds and prosthetic devices.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF), Korea Health Industry Development Institute (KHIDI)

References

  1. O'brien FJ. Biomaterials & scaffolds for tissue engineering. Mater Today. 2011;14:88-95. https://doi.org/10.1016/S1369-7021(11)70058-X
  2. Hardy JG, Lee JY, Schmidt CE. Biomimetic conducting polymer-based tissue scaffolds. Curr Opin Biotechnol. 2013;24:847-54. https://doi.org/10.1016/j.copbio.2013.03.011
  3. Balint R, Cassidy NJ, Cartmell SH. Conductive polymers: Towards a smart biomaterial for tissue engineering. Acta Biomater. 2014;10:2341-53. https://doi.org/10.1016/j.actbio.2014.02.015
  4. Pires F, Ferreira Q, Rodrigues CA, Morgado J, Ferreira FC. Neural stem cell differentiation by electrical stimulation using a cross-linked PEDOT substrate: expanding the use of biocompatible conjugated conductive polymers for neural tissue engineering. Biochim Biophys Acta BBA-Gen Subj. 2015;1850: 1158-68. https://doi.org/10.1016/j.bbagen.2015.01.020
  5. Green RA, Baek S, Poole-Warren LA, Martens PJ. Conducting polymerhydrogels for medical electrode applications. Sci Technol Adv Mater. 2016.
  6. Guimard NK, Gomez N, Schmidt CE. Conducting polymers in biomedical engineering. Prog Polym Sci. 2007;32:876-921. https://doi.org/10.1016/j.progpolymsci.2007.05.012
  7. Bendrea A-D, Cianga L, Cianga I. Review paper: progress in the field of conducting polymers for tissue engineering applications. J Biomater Appl. 2011;26:3-84. https://doi.org/10.1177/0885328211402704
  8. Guiseppi-Elie A. Electroconductive hydrogels: Synthesis, characterization and biomedical applications. Biomaterials. 2010;31:2701-16. https://doi.org/10.1016/j.biomaterials.2009.12.052
  9. Qu B, Chen C, Qian L, Xiao H, He B. Facile preparation of conductive composite hydrogels based on sodium alginate and graphite. Mater Lett. 2014;137:106-9. https://doi.org/10.1016/j.matlet.2014.08.137
  10. Peng R, Yu Y, Chen S, Yang Y, Tang Y. Conductive nanocomposite hydrogels with self-healing property. RSC Adv. 2014;4:35149-55. https://doi.org/10.1039/C4RA05381H
  11. Guarino V, Alvarez-Perez MA, Borriello A, Napolitano T, Ambrosio L. Conductive PANi/PEGDA macroporous hydrogels for nerve regeneration. Adv Healthc Mater. 2013;2:218-27. https://doi.org/10.1002/adhm.201200152
  12. Kim D, Abidian M, Martin DC. Conducting polymers grown in hydrogel scaffolds coated on neural prosthetic devices. J Biomed Mater Res A. 2004; 71:577-85.
  13. Thrivikraman G, Madras G, Basu B. Intermittent electrical stimuli for guidance of human mesenchymal stem cell lineage commitment towards neural-like cells on electroconductive substrates. Biomaterials. 2014;35:6219-35. https://doi.org/10.1016/j.biomaterials.2014.04.018
  14. Collins MN, Birkinshaw C. Hyaluronic acid based scaffolds for tissue engineering-A review. Carbohydr Polym. 2013;92:1262-79. https://doi.org/10.1016/j.carbpol.2012.10.028
  15. Prestwich GD. Hyaluronic acid-based clinical biomaterials derived for cell and molecule delivery in regenerative medicine. J Controlled Release. 2011; 155:193-9. https://doi.org/10.1016/j.jconrel.2011.04.007
  16. Segura T, Anderson BC, Chung PH, Webber RE, Shull KR, Shea LD. Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern. Biomaterials. 2005;26:359-71. https://doi.org/10.1016/j.biomaterials.2004.02.067
  17. Kogan G, Soltes L, Stern R, Gemeiner P. Hyaluronic acid: a natural biopolymer with a broad range of biomedical and industrial applications. Biotechnol Lett. 2007;29:17-25.
  18. Yoo HS, Lee EA, Yoon JJ, Park TG. Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering. Biomaterials. 2005;26:1925-33. https://doi.org/10.1016/j.biomaterials.2004.06.021
  19. Solis MA, Chen Y-H, Wong TY, Bittencourt VZ, Lin Y-C, Huang LL. Hyaluronan regulates cell behavior: a potential niche matrix for stem cells. Biochem Res Int. 2012;2012.
  20. Zhu H, Mitsuhashi N, Klein A, Barsky LW, Weinberg K, Barr ML, et al. The role of the hyaluronan receptor CD44 in mesenchymal stem cell migration in the extracellular matrix. Stem Cells. 2006;24:928-35. https://doi.org/10.1634/stemcells.2005-0186
  21. Miyake K, Underhill CB, Lesley J, Kincade PW. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J Exp Med. 1990;172:69-75. https://doi.org/10.1084/jem.172.1.69
  22. Lei Y, Gojgini S, Lam J, Segura T. The spreading, migration and proliferation of mouse mesenchymal stem cells cultured inside hyaluronic acid hydrogels. Biomaterials. 2011;32:39-47. https://doi.org/10.1016/j.biomaterials.2010.08.103
  23. Ateh D, Navsaria H, Vadgama P. Polypyrrole-based conducting polymers and interactions with biological tissues. J R Soc Interface. 2006;3:741-52. https://doi.org/10.1098/rsif.2006.0141
  24. Stewart E, Kobayashi NR, Higgins MJ, Quigley AF, Jamali S, Moulton SE, et al. Electrical stimulation using conductive polymer polypyrrole promotes differentiation of human neural stem cells: a biocompatible platform for translational neural tissue engineering. Tissue Eng Part C Methods. 2014;21: 385-93.
  25. Ahuja T, Mir IA, Kumar D. Biomolecular immobilization on conducting polymers for biosensing applications. Biomaterials. 2007;28:791-805. https://doi.org/10.1016/j.biomaterials.2006.09.046
  26. Shi Z, Gao H, Feng J, Ding B, Cao X, Kuga S, et al. In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration. Angew Chem Int Ed. 2014;53:5380-4. https://doi.org/10.1002/anie.201402751
  27. Abu-Rabeah K, Polyak B, Ionescu RE, Cosnier S, Marks RS. Synthesis and characterization of a pyrrole-alginate conjugate and its application in a biosensor construction. Biomacromolecules. 2005;6:3313-8. https://doi.org/10.1021/bm050339j
  28. Hur J, Im K, Kim SW, Kim J, Chung D-Y, Kim T-H, et al. Polypyrrole/agarosebased electronically conductive and reversibly restorable hydrogel. ACS Nano. 2014;8:10066-76. https://doi.org/10.1021/nn502704g

Cited by

  1. Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment vol.117, pp.20, 2017, https://doi.org/10.1021/acs.chemrev.7b00094
  2. Cyclic cryogelation: a novel approach to control the distribution of carbonized cellulose fibres within polymer hydrogels vol.25, pp.1, 2016, https://doi.org/10.1007/s10570-017-1544-y
  3. Incorporation of Conductive Materials into Hydrogels for Tissue Engineering Applications vol.10, pp.10, 2016, https://doi.org/10.3390/polym10101078
  4. Soft and elastic hydrogel-based microelectronics for localized low-voltage neuromodulation vol.3, pp.1, 2019, https://doi.org/10.1038/s41551-018-0335-6
  5. Hyaluronic acid promotes proliferation and migration of human meniscus cells via a CD44-dependent mechanism vol.60, pp.2, 2016, https://doi.org/10.1080/03008207.2018.1465053
  6. Three-Dimensional Printing and Injectable Conductive Hydrogels for Tissue Engineering Application vol.25, pp.5, 2016, https://doi.org/10.1089/ten.teb.2019.0100
  7. Selenophene and Thiophene-Based Conjugated Polymer Gels vol.2, pp.None, 2020, https://doi.org/10.1021/acsmaterialslett.0c00406
  8. Ionic Diffusion and Drug Release Behavior of Core-Shell-Functionalized Alginate-Chitosan-Based Hydrogel vol.5, pp.1, 2016, https://doi.org/10.1021/acsomega.9b03464
  9. Facilitated Transdermal Drug Delivery Using Nanocarriers-Embedded Electroconductive Hydrogel Coupled with Reverse Electrodialysis-Driven Iontophoresis vol.14, pp.4, 2020, https://doi.org/10.1021/acsnano.0c00007
  10. Polypyrrole-Incorporated Conducting Constructs for Tissue Engineering Applications: A Review vol.2, pp.2, 2020, https://doi.org/10.1089/bioe.2020.0010
  11. Design Strategies of Conductive Hydrogel for Biomedical Applications vol.25, pp.22, 2016, https://doi.org/10.3390/molecules25225296
  12. Stimuli‐Responsive Biomaterials: Scaffolds for Stem Cell Control vol.10, pp.1, 2021, https://doi.org/10.1002/adhm.202001125
  13. Novel pH-tunable nontoxic hydrogels of pyrrole-2-carboxylic acid and ethylenediamine derivatives: synthesis and characterization vol.60, pp.3, 2016, https://doi.org/10.1080/25740881.2020.1793200
  14. Endogenous Electric Signaling as a Blueprint for Conductive Materials in Tissue Engineering vol.3, pp.1, 2021, https://doi.org/10.1089/bioe.2020.0027
  15. 3D Photothermal Cryogels for Solar-Driven Desalination vol.13, pp.26, 2021, https://doi.org/10.1021/acsami.1c05087
  16. Programmable Assembly of π‐Conjugated Polymers vol.33, pp.46, 2016, https://doi.org/10.1002/adma.202006287
  17. Conductive Polymeric-Based Electroactive Scaffolds for Tissue Engineering Applications: Current Progress and Challenges from Biomaterials and Manufacturing Perspectives vol.22, pp.21, 2016, https://doi.org/10.3390/ijms222111543
  18. Conductive Bioimprint Using Soft Lithography Technique Based on PEDOT:PSS for Biosensing vol.8, pp.12, 2016, https://doi.org/10.3390/bioengineering8120204