생체재료학회지 (Biomaterials Research)

  • 제20권3호
  • /
  • Pages.166-173
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  • 2016
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
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Study on chemotaxis and chemokinesis of bone marrow-derived mesenchymal stem cells in hydrogel-based 3D microfluidic devices

  • Yoon, Dayoung (Center for Biomaterials, Korea Institute of Science and Technology) ;
  • Kim, Hyerim (Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University) ;
  • Lee, Eojin (Center for Biomaterials, Korea Institute of Science and Technology) ;
  • Park, Min Hee (Center for Biomaterials, Korea Institute of Science and Technology) ;
  • Chung, Seok (School of Mechanical Engineering, Korea University) ;
  • Jeon, Hojeong (Center for Biomaterials, Korea Institute of Science and Technology) ;
  • Ahn, Cheol-Hee (Research Institute of Advanced Materials (RIAM), Department of Materials Science and Engineering, Seoul National University) ;
  • Lee, Kangwon (Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University)
  • 투고 : 2016.05.26
  • 심사 : 2016.07.15
  • 발행 : 2016.09.01
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Controlling the fate of mesenchymal stems cells (MSCs) including proliferation, migration and differentiation has recently been studied by many researchers in the tissue engineering field. Especially, recruitment of stem cells to injury sites is the first and crucial step in tissue regeneration. Although significant progress has been made in the chemotactic migration of MSCs, MSC migration in three dimensional environments remains largely unknown. We developed a 3D hydrogel-based microfluidic-device to study the migration behavior of human MSCs in the presence of stromal-cell derived factor-$1{\alpha}$ (SDF-$1{\alpha}$), interleukin 8 (IL-8) and Substance P (SP) which have been utilized as chemoattractant candidates of human mesenchymal stem cells (hMSCs). Results: We systematically investigated the chemotactic migration behaviors of hMSCs and their responses to SDF-$1{\alpha}$, IL-8, and SP. SDF-$1{\alpha}$ was shown to be the most fascinating chemoattractant candidate among those factors at a certain time point. We also found that each chemokine showed different chemoattractant abilities according to their concentration. In the case of SP, this factor showed chemokinesis not chemotaxis. Especially at a $7-8{\times}10^{-8}M$ concentration range, the chemokinesis ability driven by SP was further increased. The data suggest that some factors at the optimal concentration exhibit chemokinesis or chemotaxis in a 3D hydrogel-based microfluidic device. Conclusion: In this study on chemotaxis and chemokinesis of hMSCs, the system parameters such as chemokine concentration, system stability, and 2D or 3D microenvironment are critically important to obtain meaningful results.

키워드

과제정보

연구 과제 주관 기관 : Korea Institute of Science and technology (KIST)

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