Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Diagnostic and Prognostic Relevance of Bone Marrow Microenvironment Components in Non Hodgkin's Lymphoma Cases Before and After Therapy

  • Soliman, Amira H (Department of Clinical Pathology, National Cancer Institute, Cairo University)
  • Published : 2016.12.01
⟨ Previous Next ⟩

Abstract

Objective: To evaluate stromal cells of the bone marrow microenvironment (BMM) in bone marrow trephine biopsy (BMTB) specimens, with a focus on fibronectin, tumor necrosis factor- alpha (TNF-${\alpha}$) and L-selectin in Non-Hodgkin's lymphoma (NHL) patients, before and after therapy. Materials and Methods: A total of 80 de novo NHL patients, 64 with B-cell lymphomas 80%, (follicular cell lymphoma (FCL) in 32, chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) in 12, and diffuse large cell lymphoma in 20) and 16 with T-cell lymphomas (20%) all diagnosed as T-Lymphoblastic lymphomas, were evaluated before and after therapy. For comparison, 25 age and sex matched BM donors, were included as a control group. BMTB material and BM aspirates were taken for morphological assessment of stromal cells, the plasma of these samples being examined for $TNF{\alpha}$ and L-selectin by ELISA, and fibronectin by radial immunodiffusion (RID). Results: BM stromal cells comprising reticular macrophages and fibroblasts were elevated in 53.3% of NHL cases at diagnosis, while BM fibronectin levels were decreased and BM $TNF{\alpha}$ and L-selectin were higher than in controls (p<0.05). In NHL cases, elevated values of BM $TNF{\alpha}$ and BM L-selectin were associated with signs of aggressive disease, including >1 extra nodal sites, detectable B symptoms, high grade, BM and CNS invasion, and a high International prognostic index (IPI) (p<0.05). Conclusion: BMM components, $TNF{\alpha}$, L-selectin and fibronectin, in NHL can be useful in evaluating disease activity, extent and response to treatment and as prognostic markers according to the IPI.

Keywords

References

  1. Baseggio L, Charlot C, Picollet J, Bienvenu J, Salles G (2001). High circulating tumor necrosis factor levels correlate with increased evels of soluble CD14 in patients with non-Hodgkin's lymphoma. Haematologica, 86, 774-5.
  2. Birbrair A, Frenette PS (2016). Niche heterogeneity in the bone marrow. Ann N Y Acad Sci, 1370, 82-96. https://doi.org/10.1111/nyas.13016
  3. Brenner KA, Corbett SA, Schwarzbauer JE (2000). Regulation of fibronectin matrix assembly by activated Ras in transformed cells. Oncogene, 19, 3156-63. https://doi.org/10.1038/sj.onc.1203626
  4. Campioni D, Voltan R, Tisato V, Zauli G (2014). Heterogeneity of mesenchymal stromal cells in lymphoproliferative disorders. Front Biosci (Landmark Ed), 19, 139-51 https://doi.org/10.2741/4200
  5. Cheson BD, Pfistner B, Juweid ME et al (2007). Revised response criteria for malignant lymphoma. J Clin Oncol, 25, 579-86. https://doi.org/10.1200/JCO.2006.09.2403
  6. Cheson BD, Fisher RI, Barrington SF et al (2014). Recommendations for initial evaluation, staging, and response assessment of Hodgkin and Non-Hodgkin lymphoma: The lugano classification. J Clin Oncol, 32, 3059-68. https://doi.org/10.1200/JCO.2013.54.8800
  7. Greer JP, Foerster J, Lukens JN (2003). Wintrobe's Clinical Hematology 11th edition, Williams and Wilkins, USA, pp 469-664
  8. Handerson AR (1995). Enzyme test in cardiovascular disease. In: Enzyme tests in diagnosis moss DW and rosalis B (ed), London, Hodder Arnold Publ by CRC Press, pp 90.
  9. Janowska-Wieczorek A, Majka M, Ratajczak J, Ratajczak MZ (2001). Autocrine / paracrine mechanisms in human hematopoiesis. Stem cells, 19, 99-107. https://doi.org/10.1634/stemcells.19-2-99
  10. Larsen JK (2000). Measurement of cytoplasmic and nuclear antigens in Flow Cytometry: A practical Approach. The practical approach series.3rd edn. (Ormerod MG. editor) IRL Press at Oxford University Press Oxford, pp 133-58.
  11. Li L, Neaves WB (2006). Normal stem cells and cancer stem cells: the niche matters. Cancer Res, 66, 4553-57 https://doi.org/10.1158/0008-5472.CAN-05-3986
  12. Lim EJ, Peh SC (2000). Bone marrow and peripheral blood changes in non-Hodgkin's lymphoma. Singapore Med J, 41, 279-85.
  13. Luis F, Michael J, Keeting, et al (2001). Interleukin-6 and interleukin-10 levels in chronic lymphocytic leukemia Correlation with phenotypic characteristics and outcome. Blood, 97, 256-63. https://doi.org/10.1182/blood.V97.1.256
  14. Mancini G, Garbonara AO, et al (1965). Immunochemical quantitation of FN by single radial immunodiffusion. Immunochem, 2, 235-54. https://doi.org/10.1016/0019-2791(65)90004-2
  15. McCarthy JT, Williams AW, Johnson WJ (1994). Serum beta 2-microglobulin concentration in dialysis patients: importance of intrinsic renal function. J Lab Clin Med, 123, 495-505.
  16. Meads MB, Hazlehurst LA, Dalton WS (2008). The bone marrow microenvironment as a tumor sanctuary and contributor to drug resistance. Clin Cancer Res, 14, 2519-26. https://doi.org/10.1158/1078-0432.CCR-07-2223
  17. Metkar S, Naresh KN, Manna PP, et al (2000). Circulating levels of TNF receptor super family members in lymphoid neoplasia. Am J Hematol, 65, 105-10. https://doi.org/10.1002/1096-8652(200010)65:2<105::AID-AJH3>3.0.CO;2-R
  18. Ohshima K, Haraoka S, Suzumiya J (2000). Cytoplasmic cytokines in lymphoproliferative disorders. Leuk Lymphoma, 38, 541-5. https://doi.org/10.3109/10428190009059273
  19. Seki Y, Koike T, Yano M, et al (2002). Bone marrow necrosis with dyspnea in patients with malignant lymphoma and plasma level of $TNF{\alpha}$, thrombomodulin. Am J Hematol, 70, 250-53. https://doi.org/10.1002/ajh.10136
  20. Williams JA, Nicholson GI (1963). A modified bone-biopsy drill for outpatient use. Lancet, 1, 1408.
  21. Yaris N, Buyukpamukcu M, Kansu E, Canpinar H (2001). Expression of adhesion molecules LFA-1, ICAM-1, CD44, and L-selectin in childhood non-Hodgkin lymphomas. Med Pediatr Oncol, 36, 359-64. https://doi.org/10.1002/mpo.1086