Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지

Glutaraldehyde-Mediated Synthesis of Asparaginase-Bound Maghemite Nanocomposites: Cytotoxicity against Human Colon Adenocarcinoma Cells

  • Baskar, G (Department of Biotechnology, St. Joseph's College of Engineering) ;
  • George, Garrick Bikku (Department of Biotechnology, St. Joseph's College of Engineering)
  • Published : 2016.09.01
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Abstract

Drugs processed using nanobiotechnology may be more biocompatible, with sustainable and stabilised release or action. L-asparaginase produced from fungi has many advantages for treatment of lymphocytic leukemia with lesser side effect. In the present work, maghemite nanobiocomposites of fungal asparaginase were produced using glutaraldehyde-pretreated colloidal magnetic nanoparticles. Formation of nanobiocomposites was observed using laser light scattering and confirmed by UV-visible spectrophotometry with the absorption peak at 497 nm. The specific asparaginase activity was increased from 320 U/mg with crude asparaginase to 481.5 U/mg. FTIR analysis confirmed that primary amines are the functional groups involved in binding of asparaginase on magnetic nanoparticles. The average size of the produced nanobiocomposite was found in the range of 30 nm to 40 nm using histogram analysis. The magnetic nanobiocomposite of asparaginase synthesised using glutaraldehyde showed 90.75% cytotoxicity against human colon adenocarcinoma cell lines. Hence it can be used as an active anticancer drug with an augmented level of bioavailability.

Keywords

References

  1. Baskar G, Renganathan S (2011). Optimization of media components and operating conditions for exogenous production of fungal L-asparaginase. Chiang Mai J Sci, 38, 270-79.
  2. Baskar G, Renganathan S (2012). Optimization of L-asparaginase production by Aspergillus terreus MTCC 1782 using response surface methodology and artificial neural network linked genetic algorithm. Asia-Pac J Chem Eng, 7, 212-20. https://doi.org/10.1002/apj.520
  3. Bradford M (1974). A rapid and sensitive method method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72, 248-54.
  4. Chytil P, Etrych T, Konak C, et al (2008). New HPMA copolymer-based drug carriers with covalently bound hydrophobic substituents for solid tumour targeting. J Control Release, 127, 121-30. https://doi.org/10.1016/j.jconrel.2008.01.007
  5. Kaushik NT, Snehit SM, Rasesh YP (2010). Biological synthesis of metallic nanoparticles. Nanomedicine, 6, 257-262. https://doi.org/10.1016/j.nano.2009.07.002
  6. Maeda H, Wu J, Sawa T, et al (2000). Tumor vascular permeability and the EPR effect in macromolecular therapeutics: A review. J Control Release, 65, 271-84. https://doi.org/10.1016/S0168-3659(99)00248-5
  7. Marangoni VS, Paino IM, Zucolotto V (2013). Synthesis and characterization of jacalin-goldnanoparticles conjugates as specific markers for cancer cells. Colloids Surf B: Biointerfaces, 112, 380-86. https://doi.org/10.1016/j.colsurfb.2013.07.070
  8. Matsumura Y, Maeda H (1986). A new concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs. Cancer Res, 46, 6387-92.
  9. Michaelis K, Hoffmann MM, Dreis S, et al (2006). Covalent linkage of apolipoprotein e to albumin Nanoparticles strongly enhances drug transport into the brain. J Pharmacol Exp Ther, 317, 1246-53. https://doi.org/10.1124/jpet.105.097139
  10. Mosmann, T (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  11. Parveen S, Misra R, Sahoo SK (2012). Nanoparticles: a boon to drug delivery, therapeutics, diagnostics and imaging. Nanomedicine, 8, 147-66. https://doi.org/10.1016/j.nano.2011.05.016
  12. Sinclair R, Li H, Madsen S, Dai H (2013). HREM analysis of graphite-encapsulated metallic nanoparticles for possible medical applications. Ultramicroscopy, 134, 167-74. https://doi.org/10.1016/j.ultramic.2013.05.006
  13. Steiniger SC, Kreuter J, Khalansky AS, et al (2004). Chemotherapy of glioblastomain rats using doxorubicinloaded nanoparticles. Int J Cancer, 109, 759-67. https://doi.org/10.1002/ijc.20048
  14. Tokonami S, Yamamoto Y, Shiigi H, et al (2012). Synthesis and bioanalytical applications of specific-shaped metallic nanostructures: A review. Anal Chim Acta, 716, 76-91. https://doi.org/10.1016/j.aca.2011.12.025
  15. Tong R, Yala L, Fan TM, et al (2010). The formulation of aptamer-coated paclitaxel-polylactide nanoconjugates and their targeting to cancer cells. Biomaterials, 31, 3043-53. https://doi.org/10.1016/j.biomaterials.2010.01.009
  16. Wohlfart S, Khalansky AS, Gelperina S, et al (2011). Efficient chemotherapy of rat glioblastoma using doxorubicin-loaded PLGA nanoparticles with different stabilizers. PLOS One, 6, 19121. https://doi.org/10.1371/journal.pone.0019121
  17. Wriston JC Jr, Yellin TO. (1973). L-asparaginase: A review. Adv Enzymol Relat Areas Mol Biol, 39, 185- 248.
  18. Yu BY, Kwak SY (2010). Assembly of magnetite nanoparticles into spherical mesoporous aggregates with a 3-D wormholelike porous structure. J Mater Chem, 20, 8320-28. https://doi.org/10.1039/c0jm01274b