Journal of the Korean Data and Information Science Society

한국데이터정보과학회 (The Korean Data and Information Science Society)
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한우 암소에서 근내지방도 관련 DNA 마커의 활용

Application of DNA marker related with marbling score in Hanwoo cow

  • 이윤석 (서울대학교 그린바이오과학기술연구원)
  • Lee, Yoonseok (Institute of Green Bio Science & Technology, Seoul National University)
  • 투고 : 2016.04.27
  • 심사 : 2016.05.09
  • 발행 : 2016.05.31
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초록

본 연구는 이미 한우에서 검증된 지방산 합성 효소 유전자의 g.15532 C>A, g.17924 G>A 단일염기다형성과 암소의 후대들 중 거세우의 도체성적을 이용하여 유전자 활용에 대한 방안을 모색하고자 한다. 이 단일염기다형성의 유전자형을 분석하기 위해 경북지역에서 사육된 암소 270두와 이들의 후대들 중 도체성적이 있는 거세우 270두의 게놈 DNA를 추출하여 단일염기확장법으로 유전자형을 분석하였다. 본 연구에 사용된 통계모델은 GLM의 ANCOVA 방식을 사용하였다. 한우 거세우와 암소집단에서 g.15532 C>A와 g.17924 G>A 단일염기다형성들과 근내지방도간 연관분석을 한결과 거세우집단에서 근내지방도와 유의적인 차이를 나타내었지만 암소집단에서는 유의적인 차이를 나타내지 않았다. 하지만 일반 한우농가에서 개량하는 있는 방식인 후대검정우 등급판정 결과를 이용하여 이 단일염기다형성과 분석한 결과 암소 집단에서 유의적인 차이를 나타내었다. 따라서 g.15532 C>A와 g.17924 G>A 단일염기다형성은 한우 암소 개량에 있어 후대검정우 등급판정결과와 조합하여 사용할 경우 근내지방도 개량에 효율적인 선발 보조도구 활용가치가 높을 것이라 판단되어진다.

The aim of this study was to evaluate combination of each of g.15532 C>A, g.17924 G>A SNP of FASN gene and beef quality grade of progeny in Hanwoo cow. In order to analyze the SNPs, genomic DNA was obtained from 270 Hanwoo cow and their progeny steer and g.15532 C>A and g.17924 G>A SNP was genotyped using single-based extension. Employing GLM as a statistical model. g.15532 C>A and g.17924 G>A SNP have a significant effect in Hanwoo steer but no significant effect in Hanwoo cow. Combination of each of g.15532 C>A, g.17924 G>A SNP and beef quality grade of progeny have a significant effect on marbling score in Hanwoo cow. Therefore, we suggest that g.15532 C>A and g.17924 G>A SNP contribute to genetic improvement on marbling score in Hanwoo cow.

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참고문헌

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