Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

Expression Analysis of Lily Type Lectin Isotypes in the Rock Bream, Oplegnathus fasciatus: in the Tissue, Developmental Stage and Viral Infection

  • Lee, Young Mee (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Yang, In Jung (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Noh, Jae Koo (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Kim, Hyun Chul (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Park, Choul-Ji (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Park, Jong-Won (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Noh, Gyeong Eon (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Kim, Woo-Jin (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS)) ;
  • Kim, Kyung-Kil (Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS))
  • Received : 2016.10.19
  • Accepted : 2016.12.10
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Lectins belong to the pattern-recognition receptors (PRRs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. Recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. However, this study is limited to information that the lectin is involved in the innate immune response against viral infection. In the present study, the lily-type lectin-2 and -3 of Oplegnathus fasciatus (OfLTL-2 and 3) have been presented to be included B-lectin domain and two D-mannose binding sites in the amino acid sequence that an important feature for the fundamental structure. To investigate the functional properties of OfLTLs, the tissue distribution in the healthy rock bream and temporal expression during early developmental stage analysis are performed using quantitative real-time PCR. OfLTL-2 and 3 are predominantly expressed in the liver and skin, but rarely expressed in other organ. Also, the transcripts of OfLTLs are not expressed during the early developmental stage but its transcripts are increased after immune-related organs which are fully formed. In the challenge experiment with RBIV (rock bream iridovirus), the expression of OfLTLs was increased much more strongly in the late response than the early, unlike previously known. These results suggest that OfLTLs are specifically expressed in the immune-related tissues when those organs are fully formed and it can be inferred that the more intensively involved in the second half to the virus infection.

Keywords

References

  1. Arasu A, Kumaresan V, Sathyamoorthi A, Palanisamy R, Prabha N, Bhatt P, Roy A, Thirumalai MK, Gnanam AJ, Pasupuleti M, Marimuthu K, Arockiaraj J (2013) Fish lily type lectin-1 contains ${\beta}$-prism architecture: Immunological characterization. Mol Immunol 56:497-506. https://doi.org/10.1016/j.molimm.2013.06.020
  2. Castro R, Abos B, Pignatelli J, von Gersdorff Jorgensen L, Gonzalez Granja A, Buchmann K, Tafalla C (2014) Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection. PLoS One 22:e111084.
  3. Choi KM., Shim SH, An CM, Nam BH, Jeong JM, Kim JW, Park CI (2015) Functional characterisation and expression analysis of recombinant serum amyloid P isoform 1 (RbSAP1) from rock bream (Oplegnathus fasciatus). Fish Shellfish Immunol 45:277-285. https://doi.org/10.1016/j.fsi.2015.04.021
  4. Inouye K, Yamano K, Maeno Y, Nakajima K, Matsuoka M, Wada Y, Sorimachi M (1992) Iridovirus infection of cultured red sea bream, Pagrus major. Fish Pathol 27:19-27. https://doi.org/10.3147/jsfp.27.19
  5. Kim BS, Nam BH, Kim JW, Park HJ, Song JH, Park CI (2011) Molecular characterisation and expression analysis of a fish-egg lectin in rock bream, and its response to bacterial or viral infection. Fish Shellfish Immunol 31:1201-1207. https://doi.org/10.1016/j.fsi.2011.10.024
  6. Kim JW, Park CI, Hwang SD, Jeong JM, Kim KH, Kim DH, Shim SH (2013) Molecular characterisation and expression analysis of the cathepsin H gene from rock bream (Oplegnathus fasciatus). Fish Shellfish Immunol 35:188-194. https://doi.org/10.1016/j.fsi.2013.04.016
  7. Lee JW, Kim JE, Goo IB, Hwang JA, Im JH, Choi HS, Lee JH (2015) Expression of immune-related genes during loach (Misgurnus anguillicaudatus) embryonic and early larval development. Dev Reprod 19:181-187. https://doi.org/10.12717/DR.2015.19.4.181
  8. Magnadottir B (2006) Innate immunity of fish (overview). Fish Shellfish Immunol 20:137-151. https://doi.org/10.1016/j.fsi.2004.09.006
  9. Nakajima K, Sorimachi M (1994) Biological and physicochemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major. Fish Pathol 29:29-33. https://doi.org/10.3147/jsfp.29.29
  10. Park HJ, Kim JW, Kim EG, Kim HN, Chae YS, Jeong JM, Kim DH, Park CI (2012) Molecular cloning and expression analysis of two distinct F-type lectins from the rock bream, Oplegnathus fasciatus. Dev Comp Immunol 36:230-235. https://doi.org/10.1016/j.dci.2011.05.011
  11. Park HJ, Jeong JM, Bae JS, Kim JW, An CM, Min BH, Kim SY, Myeong JI, Hwang HK, Park CI (2016) Molecular cloning and expression analysis of a new lily-type lectin in the rock bream, Oplegnathus fasciatus. Dev Comp Immunol 65:25-30. https://doi.org/10.1016/j.dci.2016.06.014
  12. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45. https://doi.org/10.1093/nar/29.9.e45
  13. Racanelli V, Rehermann B (2006) The liver as an immunological organ. Hepatology 43:54-62. https://doi.org/10.1002/hep.20984
  14. Saraiva TC, Grund LZ, Komegae EN, Ramos AD, Conceicao K, Orii NM, Lopes-Ferreira M, Lima C (2011) Nattectin a fish C-type lectin drives Th1 responses in vivo: licenses macrophages to differrentiate into cells exhibiting typical DC function. Int Immunopharmacol 11:1546-1556. https://doi.org/10.1016/j.intimp.2011.05.012
  15. Sharon N, Lis H (2004) History of lectins: from hemagglutinins to biological recognition molecules. Glycobiology 4:53-62.
  16. Suzuki Y, Tasumi S, Tsutsui S, Okamoto M, Suetake H (2003) Molecular diversity of skin mucus lectins in fish. Comp Biochem Physiol B Biochem Mol Biol 136:723-730. https://doi.org/10.1016/S1096-4959(03)00178-7
  17. Tasumi S, Yamaguchi A, Matsunaga R, Fukushi K, Suzuki Y, Nakamura O, Kikuchi K, Tsutsui S (2016) Identification and characterization of pufflectin from the grass pufferfish Takifugu niphobles and comparison of its expression with that of Takifugu rubripes. Dev Comp Immunol 59:48-56. https://doi.org/10.1016/j.dci.2016.01.007
  18. Thulasitha WS, Umasuthan N, Whang I, Nam BH, Lee J (2016) Antimicrobial response of galectin-1 from rock bream Oplegnathus fasciatus: Molecular, transcripttional, and biological characterization. Fish Shellfish Immunol 50:66-78. https://doi.org/10.1016/j.fsi.2016.01.010
  19. Umasuthan N, Revathy KS, Bathige SD, Lim BS, Park MA, Whang I, Lee J (2013) A manganese superoxide dismutase with potent antioxidant activity identified from Oplegnathus fasciatus: genomic structure and transcriptional characterrization. Fish Shellfish Immunol 34:23-37. https://doi.org/10.1016/j.fsi.2012.09.011
  20. Wassaman PM, Bleil JD, Florman HM, Greve JM, Roller RJ, Salzmann GS (1986) Nature of the mouse egg's receptor for sperm. Adv Exp Med Biol 207:55-77.
  21. Wilkins MR, Gasteiger E, Bairoch A, Sanchez JC, Williams KL, Appel RD, Hochstrasser DF (1999) Protein identification and analysis tools in the ExPASy server. Methods Mol Biol 112:531-552.
  22. Zhizhong X, Tao H, Jun L, Tianxiang G (2013) Ontogeny of the immune system in rock bream Oplegnathus fasciatus. Chin J Oceanol Limnol 31:1028-1035. https://doi.org/10.1007/s00343-013-2278-9