Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Tricin and Tricin 4'-O-(Threo-β-Guaiacylglyceryl) Ether Contents of Rice Hull (Oryza sativa L.) with Heat Treatment and Germination

열처리와 발아에 따른 왕겨(Oryza sativa L.)의 Tricin과 Tricin 4'-O-(Threo-β-Guaiacylglyceryl) Ether 함량

  • Yoon, Nara (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Jang, Gwi Yeong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Yoon Jeong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Li, Meishan (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Min Young (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Hyun Young (Division of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Junsoo (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Jeong, Heon Sang (Department of Food Science and Biotechnology, Chungbuk National University)
  • 윤나라 (충북대학교 식품생명공학과) ;
  • 장귀영 (충북대학교 식품생명공학과) ;
  • 이윤정 (충북대학교 식품생명공학과) ;
  • ;
  • 김민영 (충북대학교 식품생명공학과) ;
  • 김현영 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 이준수 (충북대학교 식품생명공학과) ;
  • 정헌상 (충북대학교 식품생명공학과)
  • Received : 2016.01.06
  • Accepted : 2016.02.05
  • Published : 2016.05.31
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Abstract

This study was conducted to evaluate the effects of heat treatment and germination on tricin and tricin 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether (TTGE) formation from rice hull (Oryza sativa L.). Heat treatments were conducted at $80{\sim}140^{\circ}C$ for 1~5 h. Germination periods were 1~6 days at $37^{\circ}C$. Germinated rough rice extracts were classified as non-filtrated, filtrated, and residue after filtration. For heat treatment, the highest contents of tricin and TTGE were 253.12 and $208.39{\mu}g/g$ at $130^{\circ}C$ after 1 h, respectively. For germination, the highest contents of tricin and TTGE in rice hull were 118.20 and $95.37{\mu}g/g$ after 2 days, respectively. In the germinated rough rice extract treatment, the highest contents of tricin and TTGE were 361.76 and $308.08{\mu}g/g$, respectively, in residue after filtration of germinated rice extract for 6 days. Therefore, the optimum conditions for tricin and TTGE formation were heat treatment at $130^{\circ}C$ for 1 h, germination for 2 days, and addition of residue after filtration of germinated rough rice extract for 6 days.

본 연구에서는 열처리 및 발아와 발아 벼로부터 추출한 조효소액에 의한 왕겨의 tricin과 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether(TTGE)의 함량 변화에 대해 살펴보았다. 열처리에 의한 왕겨의 tricin과 TTGE의 함량은 온도 및 시간이 증가함에 따라 증가하였고 $130^{\circ}C$에서 1시간 처리하였을 때 각각 253.12 및 $208.39{\mu}g/g$으로 가장 높게 나타났으며, 발아 2일차에서 각각 118.20 및 $95.37{\mu}g/g$으로 가장 높게 나타났다. 발아일수별 벼의 조효소액을 여과한 조효소액 및 여과 후 남은 잔여물을 접종한 결과 여과한 조효소액의 처리구에서는 뚜렷한 함량 차이가 없었으나 잔여물을 처리하였을 때 tricin 및 TTGE의 함량이 유의적으로 증가하였다. 특히 발아 벼 6일 차에서 각각 361.76 및 $308.08{\mu}g/g$으로 가장 많은 함량을 나타내었다. 이상의 결과로부터 tricin과 TTGE의 함량을 가장 많이 증대시킬 수 있는 조건은 발아 6일차 벼로부터 추출한 조효소액 여과 잔여물을 처리한 경우로 나타났다.

Keywords

References

  1. Lee YJ, Jung WK, Sung YJ. 2011. Investigation of the effects of environment friendly digestion process on the rice hull. J Korea TAPPI 4: 139-150.
  2. Park JH, Jin JH, Kim HJ, Park HR, Lee SC. 2005. Effect of far-infrared irradiation on the antioxidant activity of extracts from rice hulls. J Korean Soc Food Sci Nutr 34: 131-134. https://doi.org/10.3746/jkfn.2005.34.1.131
  3. Park SJ, Kim MH, Shin HM. 2005. Chemical compositions and thermal characteristics of rice husk and rice husk ash in Korea. J Biosystems Eng 30: 235-241. https://doi.org/10.5307/JBE.2005.30.4.235
  4. Oh SW, Kang CH. 1999. Studies on the physical properties of molded packaging material using rice-straw pulp. J Korean Wood Sci Technol 27: 79-87.
  5. No SY. 1998. Effective utilization methods of rice husk. J Biosystems Eng 23: 507-518.
  6. Ramarathnam N, Osawa T, Namiki M, Kawakishi S. 1989. Chemical studies on novel rice hull antioxidants. 2. Identification of isovitexin, a C-glycosyl flavonoid. J Agric Food Chem 37: 316-319. https://doi.org/10.1021/jf00086a009
  7. Rho YD, Baek NI, Lee MH. 2001. Separation and identification of natural herbicidal substance from rice hull. Kor J Weed Sci 21: 49-57.
  8. Jeong RH, Lee DY, Cho JG, Lee SM, Kang HC, Seo WD, Kang HW, Kim JY, Baek NI. 2011. A new flavonolignan from the aerial parts of Oryza sativa L. inhibits nitric oxide production in Raw 264.7 macrophage cells. J Korean Soc Appl Biol Chem 54: 865-870. https://doi.org/10.1007/BF03253174
  9. Jiao J, Zhang Y, Liu C, Liu J, Wu X, Zhang Y. 2007. Separation and purification of tricin from an antioxidant product derived from bamboo leaves. J Agric Food Chem 55: 10086-10092. https://doi.org/10.1021/jf0716533
  10. Mohanlal S, Parvathy R, Shalini V, Helen A, Jayalekshmy A. 2011. Isolation, characterization and quantification of tricin and flavonolignans in the medicinal rice Njavara (Oryza sativa L.), as compared to staple varieties. Plant Foods Hum Nutr 66: 91-96. https://doi.org/10.1007/s11130-011-0217-5
  11. Jung YS, Kim DH, Hwang JY, Yun NY, Lee YH, Han SB, Hwang BY, Lee MS, Jeong HS, Hong JT. 2014. Anti-inflammatory effect of tricin 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether, a novel flavonolignan compound isolated from Njavara on in RAW264.7 cells and in ear mice edema. Toxicol Appl Pharmacol 277: 67-76. https://doi.org/10.1016/j.taap.2014.03.001
  12. Choi Y, Lee SM, Chun J, Lee HB, Lee J. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem 99: 381-387. https://doi.org/10.1016/j.foodchem.2005.08.004
  13. Hwang CR, Oh SH, Kim HY, Lee SH, Hwang IG, Shin YS, Lee JS, Jeong HS. 2011. Chemical composition and antioxidant activity of Deoduk (Codonopsis lanceolata) and Doragi (Platycodon grandiflorum) according to temperature. J Korean Soc Food Sci Nutr 40: 798-803. https://doi.org/10.3746/jkfn.2011.40.6.798
  14. Lee YR, Kim JY, Woo KS, Hwang IG, Kim KH, Kim KJ, Kim JH, Jeong HS. 2007. Changes in the chemical and functional components of Korean rough rice before and after germination. Food Sci Biotechnol 16: 1006-1010.
  15. Kim HY, Woo KS, Hwang IG, Le YR, Jeong HS. 2008. Effects of heat treatments on the antioxidant activities of fruits and vegetables. Korean J Food Sci Technol 40: 166-170.
  16. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee J, Jeong HS. 2006. Change of Korean ginseng components with high temperature and pressure treatment. Korean J Food Sci Technol 38: 521-525.
  17. Kim MY, Lee SH, Jang GY, Park HJ, Li M, Kim S, Lee YR, Noh YH, Lee J, Jeong HS. 2015. Effects of high hydrostatic pressure treatment on the enhancement of functional components of germinated rough rice (Oryza sativa L.). Food Chem 166: 86-92. https://doi.org/10.1016/j.foodchem.2014.05.150
  18. Min B, McClung A, Chen MH. 2014. Effects of hydrothermal processes on antioxidants in brown, purple and red bran whole grain rice (Oryza sativa L.). Food Chem 159: 106-115. https://doi.org/10.1016/j.foodchem.2014.02.164
  19. Lee JH, Oh SK, Kim DJ, Yoon MR, Chun A, Choi IS, Lee JS, Kim YG. 2013. Comparison of antioxidant activities by different extraction temperatures of some commercially available cultivars of rice bran in Korea. Korean J Food & Nutr 26: 1-7. https://doi.org/10.9799/ksfan.2013.26.1.001
  20. Thompson KA, Marshall MR, Sims CA, Wei CI, Sargent SA, Scott JW. 2000. Cultivar, maturity, and heat treatment on lycopene content in tomatoes. J Food Sci 65: 791-795. https://doi.org/10.1111/j.1365-2621.2000.tb13588.x
  21. Park EJ, Ahn JJ, Kwon JH. 2013. Effect of reflux conditions on extraction properties and antioxidant activity of freeze dried-Schisandra chinensis. Korean J Food Sci Technol 45: 550-556. https://doi.org/10.9721/KJFST.2013.45.5.550
  22. Turkmen N, Sari F, Velioglu YS. 2005. The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chem 93: 713-718. https://doi.org/10.1016/j.foodchem.2004.12.038
  23. Kim HY, Hwang IG, Kim TM, Woo KS, Park DS, Kim JH, Kim DJ, Lee J, Lee YR, Jeong HS. 2012. Chemical and functional components in different parts of rough rice (Oryza sativa L.) before and after germination. Food Chem 134: 288-293. https://doi.org/10.1016/j.foodchem.2012.02.138
  24. Fowler ZL, Gikandi WW, Koffas MAG. 2009. Increased malonyl coenzyme A biosynthesis by tuning the Escherichia coli metabolic network and its application to flavanone production. Appl Environ Microbiol 75: 5831-5839. https://doi.org/10.1128/AEM.00270-09
  25. Leonard E, Chemler J, Lim KH, Koffas MA. 2006. Expression of a soluble flavone synthase allows the biosynthesis of phytoestrogen derivatives in Escherichia coli. Appl Microbiol Biotechnol 70: 85-91. https://doi.org/10.1007/s00253-005-0059-x
  26. Leonard E, Yan Y, Koffas MA. 2006. Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli. Metab Eng 8: 172-181. https://doi.org/10.1016/j.ymben.2005.11.001
  27. Leonard E, Lim KH, Saw PN, Koffas MA. 2007. Engineering central metabolic pathways for high-level flavonoid production in Escherichia coli. Appl Environ Microbiol 73: 3877-3886. https://doi.org/10.1128/AEM.00200-07
  28. Santos CN, Koffas M, Stephanopoulos G. 2011. Optimization of a heterologous pathway for the production of flavonoids from glucose. Metab Eng 13: 392-400. https://doi.org/10.1016/j.ymben.2011.02.002