Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Effect of salinity and used medium on the induction of sexual reproduction in the rotifer Brachionus rotundiformis

해수산 rotifer Brachionus rotundiformis의 유성생식유도에 관한 염분 및 기사용 배양수의 효과

  • Lee, Kyun-Woo (Marine Life & Ecosystem Division, Korea Institute of Ocean Science & Technology) ;
  • Sim, So-Myung (Marine Life & Ecosystem Division, Korea Institute of Ocean Science & Technology)
  • Received : 2016.03.14
  • Accepted : 2016.04.07
  • Published : 2016.04.30
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Abstract

This study examined the effects of salinity and medium used on the induction of sexual reproduction for the mass production of the resting eggs in the marine rotifer Brachionus rotundiformis for 9 days. After exposure to various salinities, the mixis rate in all treatments began to increase on the third day and decreased after the 4th or 5th day. The resting eggs appeared on the 4th day at 10‰, 15‰, 20‰, 25‰. In particular, the fertilization rates with 33.3% and 31.3% at 20‰ and 25‰ on day 6 were higher than those of the others, respectively. Mean mixis rate for 9 day at 0‰ and 20‰ were the highest with 25.3% and 20.2%, respectively, and the fertilization rate (14.0%) at 25‰ was the highest. In the medium experiment used, the mixis rate in all treatments were similar to the results of the salinity experiment. The resting eggs were only found at 0% and 25% of the used medium treatments. While there were no significant differences in the mean fertilization rate of all treatments, the mean mixis rate (22.3%) in the 25% treatment was higher than that of the control (0% treatment). As a result, salt stress was more effective than using the used medium to induce sexual reproduction in B. rotundiformis. The optimal salinity was considered to be 20-25‰, which caused a high mixis and fertilization rate in the rotifer. These results provide basic data for the mass production of rotifer resting eggs.

해수산 rotifer Brachionus rotundiformis의 내구란 대량생산을 위한 효과적인 유성생식자극요인을 구명하기 위해 염분자극과 기사용배양수의 효과를 9일 동안 조사하였다. 각 염분에 노출된 후 모든 실험구에서 배양 3일째부터 유성생식률이 증가하기 시작했으며 이후 배양 4-5일째 이후 감소하는 경향을 보였다. 내구란은 10‰, 15‰, 20‰, 25‰ 실험구에서 배양 4일째에 출현하였으며 특히 20, 25‰ 실험구는 배양 6일째 수정률이 각각 33.3%와 31.3%로 다른 실험구에 비해 높게 나타났다. 9일간 평균 유성생식률은 10‰와 20‰ 실험구에서 각각 25.3%와 20.2%로 높게 나타났다. 수정률은 25‰ 실험구에서 14.0%로 가장 높았다. 각 기사용배지농도에 노출된 후 모든 실험구의 유성생식률은 염분실험결과와 유사한 경향을 보였고 내구란은 0%과 25% 기사용배지에 노출되었을 때 만 출현하였다. 평균수정률은 모든 실험구가 차이를 보이지 않은 반면, 유성생식률은 25% 실험구가 22.3%로 대조구인 0%에 비해 높게 나타났다. 본 연구결과를 종합해보면, B. rotundiformis의 내구란 대량생산을 위한 효과적인 유성생식유발요인으로 기배양수자극보다 염분자극이 더 효과적이며 최적 염분은 비교적 높은 유성생식률과 수정률을 유도한 20-25‰로 판단된다. 본 연구결과는 차후 rotifer 내구란 대량생산을 위한 핵심적인 기초자료로 활용될 수 있을 것으로 판단된다.

Keywords

References

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