Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Improving 3'-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking Strategy

  • Wang, Tzi-Yuan (Biodiversity Research Center, Academia Sinica) ;
  • Tsai, Yi-Hsuan (Department of Biological Sciences and Technology, National University of Tainan) ;
  • Yu, I-Zen (Department of Biological Sciences and Technology, National University of Tainan) ;
  • Chang, Te-Sheng (Department of Biological Sciences and Technology, National University of Tainan)
  • Received : 2015.09.04
  • Accepted : 2015.12.14
  • Published : 2016.03.28
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Abstract

3'-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3'-hydroxygenistein, indicating a high potential for industrial-scale 3'-hydroxygenistein production.

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