The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Comparative study on stability and efficacy of Banhasasim-tang decoction depending on the preservation temperature and periods

반하사심탕 전탕액의 보관 온도 및 기간에 따른 안정성 및 유효성 연구

  • Jin, Seong Eun (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Ohn Soon (KM Convergence Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Jeong, Soo-Jin (KM Convergence Research Division, Korea Institute of Oriental Medicine)
  • 진성은 (한국한의학연구원 K-herb연구단) ;
  • 김온순 (한국한의학연구원 한의약융합연구부) ;
  • 서창섭 (한국한의학연구원 K-herb연구단) ;
  • 신현규 (한국한의학연구원 K-herb연구단) ;
  • 정수진 (한국한의학연구원 한의약융합연구부)
  • Received : 2016.01.27
  • Accepted : 2016.03.17
  • Published : 2016.03.31
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Abstract

This study aimed to investigate the stability and biological activities of BHSST decoction depending on the preservation temperature and periods. Methods: BHSST decoction was preserved at room temperatures (R/T, $23{\pm}1^{\circ}C$) or refrigeration ($4^{\circ}C$) for 0, 30, 60 and 90 days. To evaluate the stability of BHSST decoction, pH and sugar content were estimated. In addition, high-performance liquid chromatography (HPLC) analysis was performed to determine marker compounds of BHSST decoction. To evaluate anti-inflammatory effect, nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) productions were measured in LPS-stimulated RAW 264.7 macrophages. Antioxidant activity was examined using the assays for 3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. Results: There was no change in pH and sugar content depending on the preservation temperature and periods of BHSST decoction. Among the major components of BHSST, contents of liquiritin, baicalein and wogonin was reduced time-dependently both at R/T and $4^{\circ}C$. Inhibitory effects of BHSST decoction on NO and PGE2 productions were slightly decreased in a time-dependent manner by 90 days of preservation. In addition, BHSST decoction maintained ABTS and DPPH radical scavenging activities by 60 days while significantly reducing the activities in 90 days of preservation at R/T. By contrast, BHSST decoction had no significant change of ABTS and DPPH radical scavenging activities by 90 days at $4^{\circ}C$. Conclusions: Our results suggest that the stability and efficacy of BHSST decoction are maintained for 60 days at $4^{\circ}C$ rather than R/T.

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References

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