Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Expression of Genes Affecting Skin Coloration and Sugar Accumulation in 'Hongro' Apple Fruits at Ripening Stages in High Temperatures

고온에 의한 변색단계별 '홍로' 사과의 착색 및 당 축적 관련 유전자 발현 분석

  • Kim, Seon Ae (Department of Horticulture and Life Science, Yeungnam University) ;
  • Ahn, Soon Young (Department of Horticulture and Life Science, Yeungnam University) ;
  • Yun, Hae Keun (Department of Horticulture and Life Science, Yeungnam University)
  • 김선애 (영남대학교 원예생명과학과) ;
  • 안순영 (영남대학교 원예생명과학과) ;
  • 윤해근 (영남대학교 원예생명과학과)
  • Received : 2016.02.08
  • Accepted : 2016.03.23
  • Published : 2016.03.31
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Abstract

High temperature is one of the important environmental factors limiting cultivation of apple (Malus domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation in response to high temperature was studied in the 'Hongro' apple fruits at different developmental stages in different temperature conditions through real-time PCR. Expression of ${\hat{a}}$-amylase (BMY) and polygalacturonase (PG) genes related with sugar synthesis was higher in late ripening stages than in initial ripening stages. Expression of four genes such as phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and malate dehydrogenase (MDH), which were related with fruit skin coloration, increased gradually in apple fruits of the middle and late ripening stages. Interestingly, the expressions of all genes were highly inhibited expressed at $30-35^{\circ}C$ compared to $25^{\circ}C$ in all ripening stages. In the further work, investigation of expression levels of various genes could be conducted in the level of transcriptomics in fruits at the middle ripening stages to get meaningful information of ripening metabolism in apple in high temperatures.

고온은 사과(Malus domestica Borkh) 과실의 품질에 영향을 끼치는 가장 중요한 환경 요인 중 하나이다. 착색기의 '홍로' 사과 과실을 3단계로 구분하여 Real-time PCR을 통해 온도조건에 따른 안토시아닌 합성과 당 축적 관련 유전자의 발현 차이를 조사하였다. 당 합성관련 유전자인 ${\beta}$-amylase(BMY)와 polygalacturonase(PG)의 발현은 변색 시작단계보다 마지막 단계에서 월등히 높았다. 과피의 착색과 관련있는 phenylalanine ammonia-lyase(PAL), chalcone synthase(CHS), flavanone 3-hydroxylase(F3H)와 malate dehydrogenase(MDH)유전자는 변색 초기작단계에서는 고온 처리 24시간후에 발현이 증가하는 경향을 보였으며 변색 중간단계에서는 점차 증가하는 경향을 보였다. 변색단계별로 보았을 때 $25^{\circ}C$처리구가 다른 온도처리구보다 발현 정도가 더 높았으며, 변색 시작단계보다 마지막 단계에서의 발현이 강하게 유도되었다. 본 연구의 결과로 착색 초기단계의 과실이 고온스트레스가 가장 영향을 끼치므로 착색초기단계의 과실을 이용하여 전사체를 분석하면 분자생물학적 수준에서 사과의 성숙대사에서 유용한 정보를 얻을 수 있을 것으로 사료된다.

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References

  1. KOSAT. 2014. http://kosat.go.kr/potal/korea/kor-nw/2/7/1/index.board.
  2. Atkinson, R.G., R. Schroder, I.C. Hallett, D. Cohen, and E.A. MacRae. 2002. Overexpression of polygalacturonase in transgenic apple trees leads to a range of novel phenotypes involving changes in cell adhesion. Plant Physiol. 129:122-133. https://doi.org/10.1104/pp.010986
  3. Azuma, A., H. Yakushiji, Y. Koshita, and S. Kobayashi. 2012. Flavonoid biosynthesis-related genes in grape skin are differentially regulated by temperature and light conditions. Planta 236:1067-1080. https://doi.org/10.1007/s00425-012-1650-x
  4. Ban, Y., S. Kondo, B.E. Ubi, C. Honda, H. Bessho, and T. Moriguchi. 2009. UDP-sugar biosynthetic pathway: contribution to cyaniding 3-galactoside biosynthesis in apple skin. Planta. 230: 871-881. https://doi.org/10.1007/s00425-009-0993-4
  5. Barritt, B.H., C.R. Rom, K.R. Guelich, S.R. Drake, and M.A. Dilley. 1991. Light level influences spur quality and canopy development and light interception influence fruit production in apple. Hort Science 26:993-999.
  6. Bergqvist, J., N. Dokoozlian, and N. Ebisuda. 2001. Sunlight exposure and temperature effects on berry growth and composition of Cabernet Sauvignon and Grenache in the central San Joaquin valley of California. Am. J. Enol. Vitic. 52:1-7.
  7. Beruter, J. 1985. Sugar accumulation and changes in the activities of related enzymes during development of the apple fruit. J. Plant Physiol. 121:331-341. https://doi.org/10.1016/S0176-1617(85)80026-2
  8. Chang, S., J. Puryear, and J. Cairney. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Mol.Biol. 11:113-116. https://doi.org/10.1007/BF02670468
  9. Costa, F., C.P. Peace, S. Stella, S. Serra, S. Musacchi, M. Bazzani, S. Sansavini, and W.E. Van de Weg. 2002. QTL dynamics for fruit firmness and softening around an ethylene-dependent polygalacturonase gene in apple (Malus x domestica Borkh.). J. Exp. Bot. 1-11.
  10. Curry, E.A. 1997. Temperatures for optimal anthocyanin accumulation in apple tissue. J. Hort. Sci. 72:723-729.
  11. Honda, C., N. Kotoda, M. Wada, S. Kondo, S. Kobayashi, J. Soejima, Z. Zhang, T. Tsuda, and T. Moriguchi. 2002. Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin. Plant Physiol. Biochem. 40: 955-962. https://doi.org/10.1016/S0981-9428(02)01454-7
  12. Hulme, M., Z.C. Zaho, and T. Jiang. 1994. Recent and future climate change in East Asia. Intl. J. Climatol. 14:637-658. https://doi.org/10.1002/joc.3370140604
  13. Ju, Z., C. Liu, and Y. Yuan. 1995a. Activities of chalcone synthase and UDPGal:flavonoid 3-O-glycosyltransferase in relation to anthocyanin synthesis in apple. Sci. Hortic. 63:175-185. https://doi.org/10.1016/0304-4238(95)00807-6
  14. Ju, Z., C. Liu, Y. Yuan, C. Liu, and S. Xin. 1995b. Relationships among phenylalanine ammonia-lyase activity, simple phenol concentrations and anthocyanin accumulation in apple. Sci. Hortic. 61:215-226. https://doi.org/10.1016/0304-4238(94)00739-3
  15. Kim, S.Y., I.H. Heo, and S.H. Lee. 2010. Impacts of temperature rising on changing of cultivation area of apple in Korea. J. Kor. Assoc. Reg. Geograph.. 16:201-215. (in Korean)
  16. Kondo, S., K. Hiraoka, S. Kobayashi, C. Honda, and N. Terahara. 2002. Changes in the expression of anthocyanin biosynthetic genes during apple development, J. Am. Soc. Hort. Sci. 127:971-976.
  17. Lakso, A.N. 1994. Apple. Handbook of Environmental Physiology of Fruit Crops. 1:3-42.
  18. Lancaster, J.E. 1992. Regulation of skin color in apples. Crit. Rev. Plant Sci. 10:487-502. https://doi.org/10.1080/07352689209382324
  19. Lee, H.C. 1999. Physiologycal and ecological factors affecting fruit coloration and color enhancement in Malus domestica Borkh. cv. 'Fuji'. Ph. D. Thesis. Seoul National University, Korea. (in Korean)
  20. Lee, J.C., T. Tomana, U. Naoki, and K. Ikuo. 1979. Physiological study on the anthocyanin development in grape - I. Effect of fruit temperature on the anthocyanin development in "Kyoho" grape. Kor. J. Hort. Sci. Technol. 20:55-65. (in Korean)
  21. Lister, C.E., J.E. Walker, and J.R.L. Lancaster. 1996. Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars. J. Sci. Food Agric. 71:313-320. https://doi.org/10.1002/(SICI)1097-0010(199607)71:3<313::AID-JSFA586>3.0.CO;2-N
  22. Noro, S., H. Ichinohe, and N. Obra. 1991. Effects of controlled air temperature on development of anthocyanin, sugar and citramalic acid during maturation of the apple cultivar Starking Delicious. Bulletin of the Aomori Apple Experiment Station. 27:111-123.
  23. Park, M.Y., Y.Y. Song, H.H. Han, and D.H. Sagong. 2009. Influence of air temperature during the growing period on water core occurrence in 'Hongro' apple cultivar and the mitigation technique. Korean J. Agr. Forest Meteorol. 11:100-110. (in Korean) https://doi.org/10.5532/KJAFM.2009.11.3.100
  24. Reay, P.F. 1999. The role of low temperatures in the development of the red blush on apple fruit ('Granny Smith'). Sci. Hortic. 79:113-119. https://doi.org/10.1016/S0304-4238(98)00197-6
  25. Sagong, D.H., H.J. Kweon, M.Y. Park, Y.Y. Song, S.H. Ryu, M.J. Kim, K.H. Choi, and T.M. Yoon. 2013. Impacts of urban high temperature events on physiology of apple trees: a case study of 'Fuji'/M.9 apple trees in daegu, korea. Korean J. Agr. Forest Meteorol. 15:130-144. (in Korean) https://doi.org/10.5532/KJAFM.2013.15.3.130
  26. Saure, M.C. 1990. External control of anthocyanin formation in apple. Sci. Hortic. 42:181-218. https://doi.org/10.1016/0304-4238(90)90082-P
  27. Seo, H.H. and H.S. Park. 2003. Fruit quality of 'Tsugaru' apples influenced by meteorological elements. Korean J. Agr. Forest Meteorol. 54:218-225.
  28. Song, J.H., I.K. Kang, and D.G. Choi. 2014. Light conditions and characteristics of leaves and fruit at different canopy positions in slender-spindle 'Hongro' apple trees. Kor. J. Hort. Sci. Technol. 32:440-447.
  29. Song, K.J., J.H. Hwang, and H.K. Yun. 2003. Changes of soluble sugar and starch concentrations in fruits of apple cultivars differing in maturity. Hort. Environ. Biotechnol. 44:207-210.
  30. Steyn, W.J., D.M. Horlcroft, S.J.E. Wand, and G. Jacobs. 2004. Anthocyanin degradation in detached pome fruit with reference to preharvest red color loss and pigmentation patterns of blushed and fully red pears. J. Amer. Soc. Hort. Sci. 129:13-19.
  31. Steyn, W.J., S.J.E. Wand, D.M. Holcroft, and G. Jacobs. 2005. Red colour development and loss in pears. Acta Hortic. 671:79-85.
  32. Tomana, T. and H. Yamada. 1988a. Change in sugar composition during maturation stage of apple fruit grown at different locations. J. Jpn. Soc. Hortic. Sci. 57:178-183. https://doi.org/10.2503/jjshs.57.178
  33. Tomana, T. and H. Yamada. 1988b. Relationship between temperature and fruit quality of apple cultivars grown at different locations. J. Jpn. Soc. Hortic. Sci. 56:391-397. https://doi.org/10.2503/jjshs.56.391
  34. Ubi, B.E., C. Honda, H. Bessho, S. Kondo, M. Wada, S. Kobayashi, and T. Moriguchi. 2006. Expression analysis of anthocyanin biosynthetic genes in apple skin: Effect of UV-B and temperature. Plant Sci. 170:571-578. https://doi.org/10.1016/j.plantsci.2005.10.009
  35. Warrington, I.J., T.A. Fulton, E.A. Halligan, and H.N. de Silva. 1999. Apple fruit growth and maturity are affected by early season temperatures. J. Am. Soc. Hortic. Sci. 124(5):468-477.
  36. Yamada, H., H. Ohmura, C. Arai, and M. Terui. 1994. Effect of preharvest fruit temperature on ripening, sugar, and watercore occurrence in apples. J. Am. Soc. Hortic. Sci. 119(6):1208-1214.
  37. Yamada, H., K. Hamamoto, A. Sugiura, and T. Tomana. 1988. Effect of controlled fruit temperature on maturation of apple fruits. J. Jpn. Soc. Hortic. Sci. 57(2):173-177. https://doi.org/10.2503/jjshs.57.173
  38. Yoon, C.G., I.C. Son, and D.I. Kim. 2010. Effects of temperature during growth stage on tree growth and fruit quality in 'Fuji'/M.9 apples. J. Kor. Soc. Int. Agric. 22:143-147.