한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제24권2호
  • /
  • Pages.129-135
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  • 2016
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지
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산벚나무 가지 추출물 및 용매 분획물의 Nitric Oxide 생성 억제 효과와 여드름 원인균에 대한 항균활성

Antibacterial and Nitric Oxide Production Inhibitory Activities of Prunus sargentii Branches Extract and Its Fractions against Pathogens of Acne

  • 양선아 (동신대학교 생물자원산업화지원센터) ;
  • 표병식 (동신대학교 한약재산업학과) ;
  • 김선민 (동신대학교 한약재산업학과)
  • Yang, Sun A (Biotechnology Industrialization Center, Dongshin University) ;
  • Pyo, Byoung Sik (Deptartment of Oriental Medicine Materials, Dongshin University) ;
  • Kim, Sun Min (Deptartment of Oriental Medicine Materials, Dongshin University)
  • 투고 : 2016.02.02
  • 심사 : 2016.02.25
  • 발행 : 2016.04.30
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초록

Background: In this study, we investigated the antibacterial and nitric oxide (NO) production inhibitory activities of 75% ethanol extract of Prunus sargentii branches and its fractions against acne pathogens. Methods and Results: The antibacterial activity against acne causing pathogens was determined using the disc diffusion assay. The ethyl acetate fraction showed higher activities against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. In the DPPH radical and NO scavenging assays, the butanol fraction showed strong DPPH radical and NO scavenging abilities. These activities were related to the total polyphenol and flavonoid contents of butanol fraction. On the other hand, the chloroform and ethyl acetate fractions exhibited the highest NO production inhibitory activity in Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells compared to those exhibited by other fractions. Conclusions: The extract and its ethyl acetate fraction from the branches of P. sargentii exhibited antibacterial activity and could be used as functional materials in antimicrobial related fields. Moreover, the chloroform and ethyl acetate fractions are potential antiinflammatory agents and butanol fraction acts as an effective radical scavenger.

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참고문헌

  1. Anagnostopoulou MA, Kefalas P, Papageorgiou VP, Assimopoulou AN and Boskou D. (2006). Radical scavenging activity of various extracts and fractions of sweet orange peel(Citrus sinensis). Food Chemistry. 94:19-25. https://doi.org/10.1016/j.foodchem.2004.09.047
  2. Blois MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  3. Choi YM, Kim MH, Shin JJ, Park JM and Lee JS. (2003). The antioxidant activities of the some commercial teas. Journal of the Korean Society of Food Science and Nutrition. 32:723-727. https://doi.org/10.3746/jkfn.2003.32.5.723
  4. Cunliffe WJ, Holland DB and Jeremy A. (2004). Comedone formation: Etiology, clinical presentation and treatment. Clinics in Dermatology. 22:367-374. https://doi.org/10.1016/j.clindermatol.2004.03.011
  5. Dewick PM. (2002). Medicinal natural products. John Wiley and Sons. Chichester, England. p.149-151.
  6. Ding AH, Nathan CF and Stuehr DJ. (1988). Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages: Comparison of activating cytokines and evidence for independent production. The Journal of Immunology. 141:2407-2412.
  7. Folin O and Denis W. (1912). On phosphotungstic-phosphomolybdic compounds as color reagents. Journal of Biological Chemistry. 12:239-243.
  8. Kang GJ. (2007). Inhibitory effect of organic extracts from Prunus yedoensis Matsum barks on the atopic dermatitis-like inflammation. Master Thesis. Cheju National University. p.56-60.
  9. Ki HG, Yun SJ, Lee JB, Kim SJ, Lee SC and Won YH. (2005). Microorganism isolated from acne and their antibiotic susceptibility. Korean Journal of Dermatology. 43:871-875.
  10. Kim HJ, Heo BK, Baek SH, Park YS and Park YJ. (2006). Effects of the tea manufacture method on mineral contents and sensory evaluation in flower and young leaf of Prunus serrulata Lindl. var. spontanea Max. People, Plants and Environment. 9:26-33.
  11. Kim SS, Hyun JM, Kim KS, Park KJ, Park SM and Choi YH. (2013). Influence of essential oil in 'Shiranuhi' immature fruit on antioxidant and antimicrobial activities. Korean Journal of Medicinal Crop Science. 21:493-497. https://doi.org/10.7783/KJMCS.2013.21.6.493
  12. Kim YH. (1999). The characterization of anthocyanin pigments prepared from cherry(Prunus serrulata L. var. spontanea Max. Wils.) for the potential sources of red colorant. Journal of the Korean Society for Applied Biological Chemistry. 42:134-169.
  13. Lee ES, Ju HK, Moon TC, Lee E, Jang Y, Lee SH, Son JK, Baek SH and Chang HW. (2004). Inhibition of nitric oxide and tumor necrosis factor-${\alpha}(TNF-{\alpha}$) production by propenone compound through blockade of nuclear factor(NF)-${\kappa}B$ activation in cultured murine macrophage. Biological and Pharmaceutical Bulletin. 27:617-620. https://doi.org/10.1248/bpb.27.617
  14. Lee SS, Lee HJ and Choi DH. (2001). Studies on biological activity of wood extractives(VII): Antimicrobial and antioxidation activities of extractives from the heartwood of Prunus sargentii. Journal of the Korean Wood Science and Technology. 29:140-145.
  15. Li H and Jeong JM. (2015). Antioxidant activities of various berries ethanolic extract. Korean Journal of Medicinal Crop Science. 23:49-56. https://doi.org/10.7783/KJMCS.2015.23.1.49
  16. Marcocci L, Maguire JJ, Droy-Lefaix MT and Packer L. (1994). The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications. 201:748-755. https://doi.org/10.1006/bbrc.1994.1764
  17. Moreno MIN, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several region of Argentina. Journal of Enthropharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  18. Park EY and Yang KS. (2008). In hibition of nitric oxide production by the extracts of Hibiscus manihot. Yakhak Hoeji. 52:259-263.
  19. Park JA and Choi MO. (2011). Antimicrobial activity and antiinflammation effect to the human skin pathogens by the Rumex crispus L. root extracts. Korean Journal of Aesthetics and Cosmetology. 9:9-16.
  20. Park JM, Lee JY, Park TS, Park GH, Park KS, Kim TH, Cho YJ, Kwon OJ, Choi KI and An BJ. (2008). Biological activity investigation, and phenol compounds isolation from barks of Prunus sargentii R. Korean Journal of Medicinal Crop Science. 16:173-182.
  21. Park YJ, Kim HJ and Heo BG. (2007). Anti-microbial, antioxidant and anti-inflammation effects with of the flower and the young leaf extracts in Oriental cherry plants. Korean Society for Plansts, People and Environment. 10:43-49.
  22. Raimer SS. (2000). Managing pediatric atopic dermatitis. Clinical Pediatric. 39:1-14. https://doi.org/10.1177/000992280003900101
  23. Shin KM, Park YM, Kim IT, Hong SP, Hong JP and Lee KT. (2003). In vitro antiinflammatory activity of amygdalin in murine macrophage Raw 264.7 cells. Korean Journal of Pharmacognosy. 34:223-227.
  24. Shin MK. (2006). Clinical traditional herbalogy. Younglimsa. Seoul, Korea. p.399-400.
  25. Sivaranjani N, Rao SV and Rajeev G. (2013). Role of reactive oxygen species and antioxidants in atopic dermatitis. Journal of Clinical and Diagnostic Research. 7:2683-2685.
  26. Song JH and Lee SR. (2015). Anti-oxidant and inhibitory activity on NO production of extract and its fractions from Rosa davurica Pall. leaves. Korean Journal of Medicinal Crop Science. 23:20-26. https://doi.org/10.7783/KJMCS.2015.23.1.20
  27. Velioglu YS, Mazza G, Gao L and Oomah BD. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry. 46:4113-4117. https://doi.org/10.1021/jf9801973
  28. Yang SA, Pyo BS, Kim SM and Lee KI. (2012). Antibacterial activity and nitric oxide production inhibitory activity of the extract and its fractions from the leaves of Prunus sargentii. Korean Journal of Medicinal Crop Science. 20:308-314. https://doi.org/10.7783/KJMCS.2012.20.4.308