Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Chitosan Nanoparticle System for Improving Blood Circulation

혈행개선을 위한 키토산 나노입자화

  • Received : 2016.02.05
  • Accepted : 2016.03.28
  • Published : 2016.04.30
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Abstract

The principal objective of this study was to produce a chitosan nanoparticle (NP) system for improving blood circulation. Chitosan NPs were prepared using fucoidan and $poly-{\gamma}-glutamic$ acid (PGA), denoted as CS/Fu and CS/Fu/PGA NPs, respectively. As the chitosan concentration was increased, the activated partial thromboplastin time (APTT) of the NPs significantly increased (p<0.05). When the concentration of fucoidan and ${\gamma}-PGA$ was 5-20 and $1-10{\mu}g/mL$, respectively, the size of the CS/Fu and CS/Fu/PGA NPs was approximately 200 and 100 nm, respectively. With an increase in the fucoidan and PGA concentration, the APTT of CS/Fu and CS/Fu/PGA NPs significantly increased (p<0.05). These results suggest that CS/Fu and CS/Fu/PGA NPs could be used as a potent NP system for improving blood circulation.

혈행개선을 위한 나노전달시스템을 제조하기 위하여, 천연 양전하성 다당류인 키토산과 혈행개선 소재로 알려져 있는 푸코이단과 PGA을 이용하여 CS/Fu 및 CS/Fu/PGA 두 종류의 나노캡슐을 제조하였다. 기본 피복물질인 키토산의 농도가 증가됨에 따라 나노캡슐의 APTT의 증가로 내인성 혈액응고 활성은 증진되었으나 혈소판 응집능 또한 증가되는 경향을 나타냈다. 따라서 키토산 농도는 대조군과 혈소판 응집능이 유의적으로 차이가 나지 않으며 나노입자 제조가 가능한 최소 농도인 2 mg/mL로 고정하였다. 그 결과 CS/Fu 나노입자의 경우 푸코이단의 농도가 $5-20{\mu}g/mL$일 때 약 200 nm 크기의 입자가 균일하게 생성되었고, CS/Fu/PGA 나노입자의 경우 PGA의 농도 $1-10{\mu}g/mL$에서 약 100nm 크기의 입자가 균일하게 생성되었다. 푸코이단과 PGA 농도 증가에 따라서 나노캡슐의 내인성 혈액응고 활성은 증가되었으나, 혈소판 응집능에는 유의적 차이를 나타내지 않았다. 즉, CS/Fu과 CS/Fu/PGA 나노입자는 각각 약 200 nm와 100 nm의 작고 균일한 입자분포를 가지고 있으며, 내인성 혈액응고 활성을 나타내고 혈소판 응집능에 영향을 미치지 않기 때문에 향후 다양한 특성의 혈행개선 활성성분을 포집할 수 있는 나노전달체로써 활용될 수 있을 것으로 판단된다.

Keywords

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