Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Genotoxicity Study from the Extracts of Fermented Acanthopanax koreanum

발효 탐라오가피 추출물의 유전독성 연구

  • Cho, Myounglae (Department of Food science and Biotechnology, Kangwon National University) ;
  • Kim, Young-Hyun (Department of Food science and Biotechnology, Kangwon National University) ;
  • Kim, Jae-Min (Department of Food science and Biotechnology, Kangwon National University) ;
  • Lee, Sang-Jong (STR Biotech Co., LTD.) ;
  • Shin, Hyun Mu (University of Massachusetts Medical School) ;
  • Lee, Ok-Hwan (Department of Food science and Biotechnology, Kangwon National University)
  • 조명래 (강원대학교 식품생명공학과) ;
  • 김영현 (강원대학교 식품생명공학과) ;
  • 김재민 (강원대학교 식품생명공학과) ;
  • 이상종 ((주)에스티알바이오텍) ;
  • 신현무 (메사추세츠주립대학교의과대학) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2016.02.05
  • Accepted : 2016.02.22
  • Published : 2016.04.30
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Abstract

This study was to determine genotoxicity from the extracts of fermented Acanthopanax koreanum. The bacterial reverse mutation assay, the extracts of fermented A. koreanum did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia coli WP2uvrA with or without metabolic activation of S-9 mixture. In addition, the micronucleus formation in ICR mice, the extracts of fermented A. koreanum treated with dose of 500, 1,000 and 2,000 mg/kg did not affected micronucleated polychromatic erythrocytes (MNPCE/2,000 PCE) and polychromatic erythrocytes (PCE)/200 polychromatic erythrocyte+normochromatic erythrocyte (RBC). The cytotoxicity effects using CHO-K1 cells observed no significant changes compared with negative control group (p < 0.05). Moreover, the extracts of fermented A. koreanum did not cause a significant chromosome aberration on CHO-K1 cells in the chromosome aberration assay. Therefore, these results suggest that the extracts of fermented A. koreanum did not induce any harmful genotoxic effects.

본 연구는 발효 탐라오가피(fermented A. koreanum) 추출물의 유전독성을 연구하기 위하여, 미생물복귀돌연변이 시험, 마우스 골수세포를 이용한 소핵시험, 염색체 이상시험을 연구하였다. 미생물복귀돌연변이 연구에서 발효 탐라오가피 추출물은 Salmonella typhimurium TA98, TA100, TA1535, TA1537와 Escherichia coli WP2uvrA에 대하여 대사활성계의 존재(+S-9 Mix) 및 부재(-S-9 Mix) 하에서 돌연변이 유도를 보이지 않았다. 또한, ICR 마우스를 이용한 소핵실험에서 발효 탐라오가피 추출물은 500, 1,000, 2,000 mg/kg 농도에서 MNPCE/2,000 PCE 와 PCE/200 RBC의 소핵형성을 유발하지 않았다. 한편, CHO-K1 세포를 이용한 염색체 이상실험에서 발효 탐라오가피 추출물은 대사활성계의 존재 6시간 처리군, 대사활성계 부재 6시간 처리군 및 대사활성계 부재 24시간 처리군에서 염색체 이상을 보이지 않았다. 따라서, 본 연구결과 발효 탐라 오가피 추출물은 유전독성을 나타내지 않음을 알 수 있었다.

Keywords

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