Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Nutritional Components and Antioxidant Activity of Dry Bitter Melon (Momordica charantia L.)

건여주의 영양성분 및 항산화 활성 효과

  • Lee, Youn Ri (Department of Food and Nutrition, Daejeon Health Sciences College)
  • 이연리 (대전보건대학교 식품영양과)
  • Received : 2015.12.08
  • Accepted : 2016.02.16
  • Published : 2016.04.30
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Abstract

This study investigated the nutritional components and antioxidant activity of dry bitter melon (Momordica charantia L.). The moisture, crude protein, crude fat, crude ash, carbohydrate, and ascorbic acid contents of dry bitter melon were 6.10%, 3.31%, 1.08%, 2.31%, 87.20%, and 908.84 mg/100 g, respectively. Potassium was the most abundant mineral, followed by Mg, P, Na, Ca, Zn, Cu, and Mn, which means dry bitter melon was an alkali material. Regarding amino acid contents, dry bitter melon was rich in arginine, urea, asparagine, ${\gamma}-aminobutyric$ acid, and alanine. Total polyphenol and total flavonoid contents of dry bitter melon extract were 36.08 mg gallic acid equivalents/extract g and 15.66 mg tannic acid equivalents/extract g, respectively. The $IC_{50}$ value for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was 9.81 mg/mL for dry bitter melon ethanol extracts.

건여주의 다양한 가공식품 개발을 위하여 영양성분 및 생리활성을 분석하였다. 건여주의 영양성분으로 일반성분은 탄수화물 87.20%, 수분 6.10%, 조단백질 3.31%, 조지방 1.08% 및 조회분 2.31%였고 비타민 C 함량은 908.84 mg/100g으로 분석되었다. 무기질 중 칼륨의 함량이 가장 많이 함유되어 있었고 다음으로 Mg, P, Na, Ca, Zn, Cu, Mn 순으로 검출되었다. 가장 많이 함유된 아미노산은 arginine, urea, asparagine, ${\gamma}-aminobutyric$ acid, alanine이며, 전체 아미노산에 대한 필수아미노산 함량 비율이 37.86%로 나타났다. 건여주의 생리활성은 70% 에탄올로 추출하여 측정하였다. 총 폴리페놀 및 플라보노이드 함량이 각각 36.08, 15.66mg GAE/g으로 나타났다. 2,2-Diphenyl-1-picrylhydrazyl 라디칼 소거능의 $IC_{50}$은 9.81 mg/mL로 나타났다.

Keywords

References

  1. Han EK, Lee JY, Jung EJ, Jin YX, Chung CK. 2010. Antioxidative activities of water extracts from different parts of Taraxacum officinale. J Korean Soc Food Sci Nutr 39: 1580-1586. https://doi.org/10.3746/jkfn.2010.39.11.1580
  2. Freeman BA, Grapo JD. 1982. Biology of disease: free radicals and tissue injury. Lab Invest 47: 412-426.
  3. McCord JM. 1987. Oxygen-derived radicals: a link between reperfusion injury and inflammation. Fed Proc 46: 2402-2406.
  4. Senanayake GV, Maruyama M, Shibuya K, Sakono M, Fukuda N, Morishita T, Yukizaki C, Kawano M, Ohta H. 2004. The effects of bitter melon (Momordica charantia) on serum and liver triglyceride levels in rats. J Ethnopharmacol 91: 257-262. https://doi.org/10.1016/j.jep.2003.12.026
  5. Grover JK, Yadav SP. 2004. Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol 93: 123-132. https://doi.org/10.1016/j.jep.2004.03.035
  6. Virdi J, Sivakami S, Shahani S, Suthar AC, Banavalikar MM, Biyani MK. 2003. Antihyperglycemic effects of three extracts from Momordica charantia. J Ethnopharmacol 88: 107-111. https://doi.org/10.1016/S0378-8741(03)00184-3
  7. An SH. 2014. Quality characteristics of muffin added with bitter melon (Momordica charantia L.) powder. Korean J Food Cook Sci 30: 499-508. https://doi.org/10.9724/kfcs.2014.30.5.499
  8. Parkash A, Ng TB, Tso WW. 2002. Purification and characterization of charantin, a napin-like ribosome-inactivating peptide from bitter gourd (Momordica charantia) seeds. J Pept Res 59: 197-202. https://doi.org/10.1034/j.1399-3011.2002.00978.x
  9. Rathi SS, Grover JK, Vats V. 2002. The effect of Momordica charantia and Mucuna pruriens in experimental diabetes and their effect on key metabolic enzymes involved in carbohydrate metabolism. Phytother Res 16: 236-243. https://doi.org/10.1002/ptr.842
  10. Schmourlo G, Mendonca-Filho RR, Alviano CS, Costa SS. 2005. Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants. J Ethnopharmacol 96: 563-568. https://doi.org/10.1016/j.jep.2004.10.007
  11. Lee HJ, Moon JH, Lee WM, Lee SG, Kim AK, Woo YH, Park DK. 2012. Charantin contents and fruit characteristics of bitter gourd (Momordica charantia L.) accessions. J Bio- Environ Control 21: 379-384. https://doi.org/10.12791/KSBEC.2012.21.4.379
  12. Kubola J, Siriamornpun S. 2008. Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chem 110: 881-890. https://doi.org/10.1016/j.foodchem.2008.02.076
  13. Beloin N, Gbeassor M, Akpagana K, Hudson J, de Soussa K, Koumaglo K, Arnason JT. 2005. Ethnomedicinal uses of Momordica charantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity. J Ethnopharmacol 96: 49-55. https://doi.org/10.1016/j.jep.2004.08.009
  14. Lee SY, Eom SH, Kim YK, Park NI, Park SU. 2009. Cucurbitane- type triterpenoids in Momordica charantia Linn. J Med Plants Res 3: 1264-1269.
  15. Choi JR, Choi JM, Lee S, Cho KM, Cho EJ, Kim HY. 2014. The protective effects of protocatechuic acid from Momordica charantia against oxidative stress in neuronal cells. Kor J Pharmacogn 45: 11-16.
  16. Lee HH, Cheong MJ, Huh J, Song SY, Boo HO. 2009. Effects of Momordica charantia L. water extracts on the rat liver and kidney with acute toxicated by lead. Korean J Microscopy 39: 355-363.
  17. Park JY, Heo YR. 2011. Effects of bitter melon (Momordica charantia) extracts on body weight change and lipid composition in C57/BL6J mice fed high fat diet. J Hum Ecol 21: 113-121.
  18. AOAC. 2000. Official methods of analysis of AOAC International. 17th ed. Association of Analytical Communities, Gaithersburg, MD, USA. p 1-26.
  19. Kim DO, Jeong SW, Lee CY. 2003. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 81: 321-326. https://doi.org/10.1016/S0308-8146(02)00423-5
  20. Jeong CH, Kim JH, Shim KH. 2006. Chemical components of yellow and red onion. J Korean Soc Food Sci Nutr 35: 708-712. https://doi.org/10.3746/jkfn.2006.35.6.708
  21. Jeong CH, Ko WH, Cho JR, Ahn CG, Shim KH. 2006. Chemical components of Korean paprika according to cultivars. Korean J Food Preserv 13: 43-49.
  22. Folin O, Denis W. 1915. A colorimetric method for the determination of phenols (and phenol derivatives) in urine. J Biol Chem 22: 305-308.
  23. Kang MS, Kim SY, Lee YH, Choi JW, Baek OH, Han HK, Kim SN, Kim JB, Park HJ, Cho YS. 2011. Analysis of nutritional components of Euonymus sieboldiana leaves. J East Asian Soc Dietary Life 21: 918-923.
  24. Dahl LK, Leitl G, Heine M. 1972. Influence of dietary potassium and sodium/potassium molar ratios on the development of salt hypertension. J Exp Med 136: 318-330. https://doi.org/10.1084/jem.136.2.318
  25. Jackson MJ, Edwards RHT, Symons MCR. 1985. Electron spin resonance studies of intact mammalian skeletal muscle. Biochim Biophys Acta 847: 185-190. https://doi.org/10.1016/0167-4889(85)90019-9
  26. Park PS. 1989. Investigation of vitamin C in the lines of bitter gourd (Momordica Charantia L.). J Chinju Nat Agri & For Jr Coll 24: 235-238.
  27. Lee G, You Y, Hwang K, Lee J, Lee HJ, Jun W. 2012. Physicochemical characteristics and antioxidant activities of Luffa cylindrica (L.) Roem. J Korean Soc Food Sci Nutr 41: 733-738. https://doi.org/10.3746/jkfn.2012.41.6.733
  28. Tannen RL. 1983. Effects of potassium on blood pressure control. Ann Intern Med 98: 773-780. https://doi.org/10.7326/0003-4819-98-5-773
  29. Goto A, Tobian L, Iwai J. 1981. Potassium feeding reduces hyperactive central nervous system pressor responses in Dahl salt-sensitive rats. Hypertension 3: I128-I134. https://doi.org/10.1161/01.HYP.3.3_Pt_2.I128
  30. Macgregor GA, Markandu ND, Smith SJ, Banks RA, Sagnella GA. 1982. Moderate potassium supplementation in essential hypertension. Lancet 320: 567-570. https://doi.org/10.1016/S0140-6736(82)90657-2
  31. http://www.ncbi.nlm.nih.gov/books/NBK234922/ (accessed Dec 2015).
  32. Oh SH, Kim SH, Moon YJ, Choi WG. 2002. Changes in the levels of ${\gamma}$-aminobutyric acid and some amino acids by application of a glutamic acid solution for the germination of brown rices. Korean J Biotechnol Bioeng 17: 49-53.
  33. Xing SG, Jun YB, Hau ZW, Liang LY. 2007. Higher accumulation of ${\gamma}$-aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max (L.) Merr. roots. Plant Physiol Biochem 45: 560-566. https://doi.org/10.1016/j.plaphy.2007.05.007
  34. Lim SD, Kim KS. 2009. Effects and utilization of GABA. Korean J Dairy Sci Technol 27: 45-51.
  35. Sato M, Ramarathnam N, Suzuki Y, Ohkubo T, Takeuchi M, Ochi H. 1996. Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different sources. J Agric Food Chem 44: 37-41. https://doi.org/10.1021/jf950190a
  36. Fitzpatrick DF, Hirschfield SL, Coffey RG. 1993. Endothelium- dependent vasorelaxing activity of wine and other grape products. Am J Physiol 265: H774-H778.
  37. Yoon S, Kwak HK, Kim YK, Kim HK, Park MS, Yeum KJ, Oh HS, Lee MJ, Lee JH, Ji GE. 2006. Functional foods. Life Science Publishing Co., Seoul, Korea. p 222-231.
  38. Jia Z, Tang M, Wu J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64: 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  39. Hertog MGL, Hollman PCH, Katan MB. 1992. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agric Food Chem 40: 2379-2383. https://doi.org/10.1021/jf00024a011
  40. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338.
  41. Kang YH, Cha HS, Kim HM, Park YK. 1997. The nitrite scavenging and electron donating ability of pumpkin extracts. Korean J Food & Nutr 10: 31-36.
  42. Park JS, Kim HS, Chin KB. 2012. The antioxidant activity of Yacon (Polymnia sonchifoliaty) and its application to the pork patties as a natural antioxidant. Korean J Food Sci Ani Resour 32: 190-197. https://doi.org/10.5851/kosfa.2012.32.2.190
  43. Lee JH, Chin KB. 2012. Evaluation of antioxidant activities of red beet extracts, and physicochemical and microbial changes of ground pork patties containing red beet extracts during refrigerated storage. Korean J Food Sci An 32: 497-503. https://doi.org/10.5851/kosfa.2012.32.4.497
  44. Choi PS, Kim HS, Chin KB. 2013. Antioxidant activities of water or methanol extract from cherry (Prunus yedoensis) and its utilization to the pork patties. Korean J Food Sci An 33: 268-275. https://doi.org/10.5851/kosfa.2013.33.2.268
  45. Moon JS, Kim SJ, Park YM, Hwang IS, Kim EH, Park JW, Park IB, Kim SW, Kang SG, Park YK, Jung ST. 2004. Antimicrobial effect of methanol extracts from some medicinal herbs and content of phenolic compounds. Korean J Food Preserv 11: 207-213.
  46. Kim H, Shin H, Hwang D, Lee J, Jeong H, Kim D. 2015. Functional cosmetic characteristics of Momordica charantia fruit extract. Korean Chem Eng Res 53: 289-294. https://doi.org/10.9713/kcer.2015.53.3.289
  47. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 232-239.

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