Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Study on Distribution of Oct4 Expression and Change of Apoptosis in Nuclear Transfer Blastocyst using Oct4-Transfected Mesenchymal Stem Cells

Oct4-Transfection한 중간엽줄기세포 유래 핵이식 배반포의 Oct4 발현 분포 및 세포 자멸사의 변화에 관한 연구

  • Lee, Won-Jae (Dept. of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University / Research Institute of Life Sciences, Gyeongsang National University) ;
  • Lee, Jeong-Hyeon (Dept. of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University / Research Institute of Life Sciences, Gyeongsang National University) ;
  • Rho, Gyu-Jin (Dept. of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University / Research Institute of Life Sciences, Gyeongsang National University) ;
  • Lee, Sung-Lim (Dept. of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University / Research Institute of Life Sciences, Gyeongsang National University)
  • 이원재 (경상대학교 수의과대학 수의학과, 경상대학교 생명과학연구원) ;
  • 이정현 (경상대학교 수의과대학 수의학과, 경상대학교 생명과학연구원) ;
  • 노규진 (경상대학교 수의과대학 수의학과, 경상대학교 생명과학연구원) ;
  • 이성림 (경상대학교 수의과대학 수의학과, 경상대학교 생명과학연구원)
  • Received : 2015.12.03
  • Accepted : 2016.03.29
  • Published : 2016.03.31
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Abstract

There are various factors i.e. donor cell type, culture system as well as technical procedures which influence the pre-implantation embryonic development; however, may attempts have been made and still it is under investigation to improve the cloning efficiency using somatic cell nuclear transfer technique. It is has been investigated that stem cells like mesenchymal stem cell are able to more efficiently reprogram and reactivate the expression of early embryonic genes to promote nuclear transfer efficiency. In addition, Oct4 expression plays a pivotal role in early embryo development. In the present study, we investigated distribution of Oct4 expression and changes of apoptosis and total cell number in nuclear transfer blastocyst after using Oct4 transfected bone marrow stem cell as donor cells. Although Oct4-RFP expression was observed across blastocyst, more concentrated intensity was shown at hatched region in blastocyst on day 7. Reduction of apoptotic bodies was revealed in Oct4 transfected blastocyst by TUNEL staining, however, there was no significant difference in total cell number between Oct4 transfected and non-transfected nuclear transfer embryos. In conclusion, Oct4 transfected donor cells exhibited higher expression in hatching sight in day 7 blastocyst and were able to prevent apoptosis compared to non-transfected donor cells.

Keywords

References

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