The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Oleanolic acid induced autophagic cell death in hepatocellular carcinoma cells via PI3K/Akt/mTOR and ROS-dependent pathway

  • Shi, Yang (Department of General Surgery, Affiliated Hospital of Xuzhou Medical College) ;
  • Song, Qingwei (Department of General Surgery, Affiliated Hospital of Xuzhou Medical College) ;
  • Hu, Dianhe (Department of General Surgery, Affiliated Hospital of Xuzhou Medical College) ;
  • Zhuang, Xiaohu (Department of General Surgery, Affiliated Hospital of Xuzhou Medical College) ;
  • Yu, Shengcai (Department of General Surgery, Affiliated Hospital of Xuzhou Medical College) ;
  • Teng, Dacai (Department of Basic Medical, Xuzhou Medical College)
  • Received : 2015.08.09
  • Accepted : 2015.09.11
  • Published : 2016.05.01
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Abstract

Oleanolic acid (OA) has a wide variety of bioactivities such as hepatoprotective, anti-inflammatory and anti-cancer activity and is used for medicinal purposes in many Asian countries. In the present study, the effect of OA on induction of autophagy in human hepatocellular carcinoma HepG2 and SMC7721 cells and the related mechanisms were investigated. MTT assay showed that OA significantly inhibited HepG2 and SMC7721 cells growth. OA treatment enhanced formation of autophagic vacuoles as revealed by monodansylcadaverine (MDC) staining. At the same time, increasing punctuate distribution of microtubule-associated protein 1 light chain 3 (LC3) and an increasing ratio of LC3-II to LC3-I were also triggered by OA incubation. In addition, OA-induced cell death was significantly inhibited by autophagy inhibitors 3-methyladenine (3-MA) and chloroquine (CQ) pretreatment. And we found out that OA can suppress the PI3K/Akt1/mTOR signaling pathway. Furthermore, our data suggested that OA-triggered autophagy was ROS-dependent as demonstrated by elevated cellular ROS levels by OA treatment. When ROS was cleared by N-acetylcysteine (NAC), OA-induced LC3-II convertsion and cell death were all reversed. Taken together, our results suggest that OA exerts anticancer effect via autophagic cell death in hepatocellular carcinoma.

Keywords

References

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