Asian Pacific Journal of Cancer Prevention

  • 제17권3호
  • /
  • Pages.1089-1092
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  • 2016
  • /
  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Novel Nonsense Variants c.58C>T (p.Q20X) and c.256G>T (p.E85X) in the CHEK2 Gene Identified dentified in Breast Cancer Patients from Balochistan

  • Baloch, Abdul Hameed (Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences Uthal) ;
  • Khosa, Ahmad Nawaz (Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences Uthal) ;
  • Bangulzai, Nasrullah (Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences Uthal) ;
  • Shuja, Jamila (Center for Nuclear Medicine and Radiotherapy (CENAR)) ;
  • Naseeb, Hafiz Khush (Center for Nuclear Medicine and Radiotherapy (CENAR)) ;
  • Jan, Mohammad (Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences Uthal) ;
  • Marghazani, Illahi Bakhsh (Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences Uthal) ;
  • Kakar, Masood-ul-Haq (Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences Uthal) ;
  • Baloch, Dost Mohammad (Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture, Water and Marine Sciences Uthal) ;
  • Cheema, Abdul Majeed (Institute of Molecular Biology and Biotechnology, The University of Lahore) ;
  • Ahmad, Jamil (Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS))
  • 발행 : 2016.04.11
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초록

Breast cancer is the most commonly occurring and leading cause of cancer deaths among women globally. Hereditary cases account 5-10% of all the cases and CHEK2 is considered as a moderate penetrance breast cancer risk gene. CHEK2 plays a crucial role in response to DNA damage to promote cell cycle arrest and repair DNA damage or induce apoptosis. Our objective in the current study was to analyze mutations in the CHEK2 gene related to breast cancer in Balochistan. A total of 271 individuals including breast cancer patients and normal subjects were enrolled. All 14 exons of CHEK2 were amplified and sequenced. The majority of the patients (>95%) had invasive ductal carcinomas (IDCs), 52.1% were diagnosed with tumor grade III and 56.1% and 27.5% were diagnosed with advance stages III and IV. Two novel nonsense variants i.e. c.58C>T (P.Q20X) and c.256G>T (p.E85X) at exon 1 and 2 in two breast cancer patients were identified in the current study. Both the variants identified were novel and have not been reported elsewhere.

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피인용 문헌

  1. Identification and analysis of CHEK2 germline mutations in Chinese BRCA1/2-negative breast cancer patients vol.169, pp.1, 2018, https://doi.org/10.1007/s10549-018-4673-6