식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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서향에서 분리한 신종 포티바이러스(Daphne Mottle Virus)의 동정

Identification of Daphne Mottle Virus Isolated from Daphne odora, a New Member of the Genus Potyvirus

  • 박충열 (경북대학교 응용생명과학부) ;
  • 박정안 (농림축산검역본부 식물검역기술개발센터) ;
  • 이부자 (농림축산검역본부 식물검역기술개발센터) ;
  • 박상민 (경북대학교 응용생명과학부) ;
  • 이홍규 (경북대학교 응용생명과학부) ;
  • 김정선 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 윤영남 (농촌진흥청 국립식량과학원 남부작물부) ;
  • 서상재 (경북대학교 응용생명과학부) ;
  • 이수헌 (경북대학교 응용생명과학부)
  • Park, Chung Youl (School of Applied Biosciences, Kyungpook National University) ;
  • Park, Jungan (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Lee, Boo-Ja (Plant Quarantine Technology Center, Animal and Plant Quarantine Agency) ;
  • Bak, Sangmin (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Hong-Kyu (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Jeong-Sun (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Yoon, Youngnam (Functional Crop Resource Development Division, National Institute of Crop Science (NICS), Rural Development Administration) ;
  • Suh, Sang Jae (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
  • 투고 : 2015.11.15
  • 심사 : 2016.03.16
  • 발행 : 2016.03.31
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초록

2014년, 국내 4개 지역에서 바이러스와 같은 병징을 보이는 서향 잎에서 신종 potyvirus를 분리하였다. 병징을 보이는 잎에서 추출한 즙액을 DN법(direct negative stain)으로 전자현미경을 이용해 관찰한 결과 사상형 형태의 입자가 관찰되었다. RT-PCR 검출 결과 3점의 시료에서 Cucumber mosaic virus와 potyvirus에 대하여 양성반응을 보였으며, 1점의 시료에서는 potyvirus 양성반응을 보였다. HC-Pro, CI, CP 유전자 일부를 BLAST 분석한 결과 각각 Daphne mosaic virus와 76%, 72%, 72%의 높은 뉴클레오티드 상동성을 보였다. 신종 potyvirus는 국부병반을 나타내는 지표식물(붉은명아주)을 이용하여 분리하였다. 본 논문에서는 서향에서 신종 potyvirus를 동정하였으며, 본 바이러스를 Daphne mottle virus (DapMoV)로 명명하였다.

A new poty-like virus was isolated from plants of winter daphne (Daphne odora) that showed virus-like symptoms on leaves, from four regions of Korea during 2014. Filamentous-shaped particles were observed by transmission electron microscopy of preparations extracted from symptomatic leaves and examined by the direct negative stain method. RT-PCR assay showed that three samples were positive for both Cucumber mosaic virus and potyvirus, and only one sample was positive for potyvirus only. A BLAST comparison to partial sequences from helper-component proteinase, cylindrical inclusion and coat protein genes detected the highest nucleotide identity of 76%, 72%, and 72% with Daphne mosaic virus, respectively, levels below the potyvirus species discrimination threshold. The new potyvirus was isolated using indicator plants (Chenopodium amaranticolor), in which local lesions were produced. In this study, we identified a novel potyvirus from winter daphne, which we have named Daphne mottle virus (DapMoV).

키워드

참고문헌

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피인용 문헌

  1. The first complete sequence and genome structure of daphne virus Y vol.161, pp.10, 2016, https://doi.org/10.1007/s00705-016-2939-z