Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Distribution of Bacterial Angular Leaf Spot of Strawberry and Characterization of Xanthomonas fragariae Strains from Korea

한국의 딸기세균모무늬병 발생분포 및 딸기세균모무늬병균 특성조사

  • Yoon, Myung-Ju (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Myung, Inn-Shik (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Jae-Yeon (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, You-Shin (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Yong-Hwan (Disaster Management Division, Extension Service Bureau, Rural Development Administration) ;
  • Kim, Dae-Young (Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Lee, Young-Ki (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • 윤명주 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 명인식 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이재연 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 김유신 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이용환 (농촌진흥청 농촌지원국 재해대응과) ;
  • 김대영 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 이영기 (농촌진흥청 국립농업과학원 작물보호과)
  • Received : 2015.10.29
  • Accepted : 2016.03.17
  • Published : 2016.03.31
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Abstract

Nationwide survey for angular leaf spot (ALS) of strawberry caused by Xanthomonas fragariae, a quarantine disease in Korea, was performed in November 2012. In the survey, ALS was observed in eighty eight farmers' fields of Sukok, Jinju and Okjong, Hadong in Gyeongnam Province, and one field in Namwon of Jeollabuk Province. The infected field of Namwon closed immediately after the disease diagnosed ALS. In detailed survey of Sukok and Okjong areas during February 2012 to January 2015, ALS occurrence decreased from 45% farmer's fields on December 2012 to 5% on January 2015, and from 38% on November 2013 to 5% on January 2015, respectively. Phenotypic characteristics of the Korean strains were similar to those of the type strain of X. fragariae. A multilocus sequence analysis of Korean strains of X. fragariae was conducted using four genes; dnaK, fyuA, gyrB, and rpoD. All the Korean strains had the same sequences of the four genes. The concatenated sequences of the Korean strains shared 100% with that of the type strain of X. fragariae. All strawberry cultivars tested were susceptible to the strains of X. fragariae two weeks after inoculation. The inoculated sites were necrosis and expanded, which were rated 4 based on evaluation of inoculation site.

2012년 11월 국가관리세균병인 딸기세균모무늬병 발생 분포를 조사하였다. 충청남도와 제주도를 제외한 전국 1,482농가포장을 조사한 결과, 경남 진주시 수곡면, 하동군 옥종면 88농가포장과 전북 남원 1농가포장에서 병이 발생되었다. 남원에서 발생된 포장은 병 진단 후 폐원되었다. 2012년 2월에서 2015년 1월까지 경남 진주시 수곡면과 하동군 옥종면의 딸기세균모무늬병 발생을 조사하였다. 2012년 12월 조사에서 수곡면은 45% 발생 후 감소하여 2015년 1월까지 약 5% 발생되었고, 옥종면은 2013년 11월 38% 발생후 감소하여 2015년 1월 약 5% 발생하였다. 분리된 세균의 특성을 조사하였다. 생리생확학적 특성은 X. fragariae 대표 균주와 일치하였다. 딸기세균모무늬병균의 dnaK (940 bp), fyuA (698 bp), gyrB (865 bp), rpoD (873 bp) 유전자들을 대상으로 3374 bp 염기의 MLSA 분석 결과를 딸기세균모무늬병 대표 균주와 비교한 결과 모든 유전자는 100% 일치하였다. X. fragariae BC3191과 BC3195에 대한 딸기 품종의 저항성을 조사하였다. 조사된 모든 품종은 딸기세균모무늬병에 감수성이었다. 모든 품종들은 접종 2주 후 접종 부위가 괴저되고 확장되어 저항성 등급 4로 평가되었다.

Keywords

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