Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Oral Toxicity Study and Skin Sensitization Test of a Cricket

  • Ryu, Hyeon Yeol (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL)) ;
  • Lee, Somin (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL)) ;
  • Ahn, Kyu Sup (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL)) ;
  • Kim, Hye Jin (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL)) ;
  • Lee, Sang Sik (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL)) ;
  • Ko, Hyuk Ju (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL)) ;
  • Lee, Jin Kyu (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL)) ;
  • Cho, Myung-Haing (Laboratory of Toxicology, College of Veterinary Medicine, Seoul National University) ;
  • Ahn, Mi Young (Department of Agricultural Biology, National Academy of Agricultural Science (RDA)) ;
  • Kim, Eun Mi (Korea Food Research Institute) ;
  • Lim, Jeong Ho (Korea Food Research Institute) ;
  • Song, Kyung Seuk (Toxicity Evaluation Center, Korea Conformity Laboratories (KCL))
  • Received : 2015.11.03
  • Accepted : 2016.01.27
  • Published : 2016.04.15
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Abstract

Crickets have been attracting considerable interest in the field of nutrition and toxicology due to the global exhaustion of food resulting from a growing population. The cricket is normally eaten in several countries after roasting, similar to the grasshopper; however, safety evaluation data on cricket powder is limited. Here, we performed general toxicity studies of cricket powder including a single, 2-week repeated dose range evaluation test, a 13-week repeated oral dose toxicity test in Sprague-Dawley rats, a single oral dose toxicity test in Beagle dogs, and a skin sensitization test in guinea pigs following the Organization for Economic Cooperation and Development test guidelines 406 and 408 in addition to Good Laboratory Practice. To investigate the NOAEL and target organs of cricket powder, Sprague-Dawley rats were allocated to 4 groups: vehicle control, 1,250 mg/kg, 2,500 mg/kg, 5,000 mg/kg dose test groups and cricket powder was administered over 13 weeks after single dose and dose range finding studies in rats based on the results of the single oral administration toxicity study in rats and Beagle dogs. The results of the study showed that the NOAEL of cricket powder was over 5,000 mg/kg for both sexes of rats without adverse effects in a 13-week repeated oral toxicity study and there was no skin hypersensitivity reaction. Therefore, our results reveal that crickets can be widely used as a new substitute food or nutrient resource.

Keywords

References

  1. Ahn, M.Y., Lee, Y.W., Ryu, K.S., Lee, H.S., Kim, I.S., Kim, J.W. and Lim, S.S. (2004) Effects of water and methanol extracts of cricket (Gryllus bimaculatus) on alcohol metabolism. Korean J. Pharmacogn., 35, 175-178.
  2. Ahn, M.Y., Hwang, J.S. and Yun, E.Y. (2015) Gene expression profiling of glycosaminoglycan drived from G. bimaculatus in high fat dieted rat. FASEB J., 29, LB152. https://doi.org/10.1096/fj.14-258541
  3. Ahn, M.Y., Han, J.W., Hwang, J.S., Yun, E.Y. and Lee, B.M. (2014) Anti-inflammatory effect of glycosaminoglycan derived from Gryllus bimaculatus (a type of cricket, insect) on adjuvant-treated chronic arthritis rat model. J. Toxicol. Environ. Health Part A, 77, 1332-1345. https://doi.org/10.1080/15287394.2014.951591
  4. Kim, I.S., Ahn, M.Y., Ryu, K.S. and Lee, B.M. (2002) Acute oral toxicity of G. bimaculatus in rats. J. Toxicol. Pub. Health, 18, 397-400.
  5. OECD (1992) Test No. 406: Skin sensitisation in OECD Guidelines for the Testing of Chemicals, Section 4. Available from: http://www.oecd-ilibrary.org/environment/test-no-406-skinsensitisation_9789264070660-en (Accessed Dec. 27, 2012).
  6. OECD (1998) Test No. 408: Repeated Dose 90-Day Oral Toxicity Study in Rodents in OECD Guidelines for the Testing of Chemicals, Section 4. Available from: http://www.oecd-ilibrary.org/environment/test-no-408-repeated-dose-90-day-oraltoxicity-study-in-rodents_9789264070707-en (Accessed Dec. 27, 2012).
  7. Basketter, D.A. and Gerberick, G.F. (1996) An interlaboratory evaluation of the Buehler test for the identification and classification of skin sensitizers. Contact Derm., 35, 146-151. https://doi.org/10.1111/j.1600-0536.1996.tb02332.x
  8. Ramos-Elorduy, J. (2002) Edible insects of chiapas, Mexico. Ecol. Food Nutr., 41, 271-299. https://doi.org/10.1080/03670240214081
  9. Hanboonsong, Y., Jamjanya, T. and Durst, P.B. (2013) Sixlegged livestock: edible insect farming, collection and marketing in Thailand, FAO, Bangkok, p. 5.
  10. Rumpold, B.A. and Schluter, O.K. (2013) Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res., 57, 802-823. https://doi.org/10.1002/mnfr.201200735
  11. van Huis, A. (2013) Potential of insects as food and feed in assuring food security. Annu. Rev. Entomol., 58, 563-583. https://doi.org/10.1146/annurev-ento-120811-153704
  12. Wang, D., Bai, Y.Y., Li, J.H. and Zhang, C.X. (2004) Nutritional value of the field cricket (Gryllus testaceus Walker). Insect Sci., 11, 275-283. https://doi.org/10.1111/j.1744-7917.2004.tb00424.x
  13. Oonincx, D.G., van Itterbeeck, J., Heetkamp, M.J., van den Brand, H., van Loon, J.J. and van Huis, A. (2010) An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption. PLoS One, 5, e14445.. https://doi.org/10.1371/journal.pone.0014445
  14. Steinfeld, H., Gerber, P., Wassenaar, T., Castel, V., Rosales, M. and Haan, C.D. (2006) Livestock's long shadow: environmental issues and options, FAO, Rome, pp. 82-83, 151-152.
  15. Kinyuru, J.N., Mogendi, J.B., Riwa, C.A. and Ndung'u, N.W. (2015) Edible insects-a novel source of essential nutrients for human diet: Learning from traditional knowledge. Animal Frontiers, 5, 14-19.
  16. Michaelsen, K.F., Hoppe, C., Roos, N., Kaestel, P., Stougaard, M., Lauritzen, L., Molgaard, C., Girma, T. and Friis, H. (2009) Choice of foods and ingredients for moderately malnourished children 6 months to 5 years of age. Food Nutr. Bull., 30, S343-S404. https://doi.org/10.1177/15648265090303S303
  17. Huis, V.A., Itterbeeck, V.J., Klunder, H., Mertens, E., Halloran, A., Muir, G. and Vantomme, P. (2013) Edible insects: future prospects for food and feed security, FAO, Rome, pp. 67-68.
  18. McLean, E., Cogswell, M., Egli, I., Wojdyla, D. and Benoist, B. (2009) Worldwide prevalence of anaemia, WHO vitamin and mineral nutrition information system, 1993-2005. Public Health Nutr., 12, 444-454. https://doi.org/10.1017/S1368980008002401
  19. Pasricha, S.R., Drakesmith, H., Black, J., Hipgrave, D. and Biggs, B.A. (2013) Control of iron deficiency anemia in lowand middle-income countries. Blood, 121, 2607-2617. https://doi.org/10.1182/blood-2012-09-453522
  20. Kassebaum, N.J., Jasrasaria, R., Naghavi, M., Wulf, S.K., Johns, N., Lozano, R., Regan, M., Weatherall, D., Chou, D.P., Eisele, T.P., Flaxman, S.R., Pullan, R.L., Brooker, S.J. and Murray, C.J. (2014) A systematic analysis of global anemia burden from 1990 to 2010. Blood, 123, 615-624. https://doi.org/10.1182/blood-2013-06-508325
  21. Yamada, T., Alpers, D.H., Kalloo, A.N., Kaplowitz, N., Owyang, C. and Powell, D.W. (2009) Textbook of gastroenterology (5th edition), Wiley-blackwell pub, Chichester, pp. 17-18.
  22. Kumar, P. and Clark, M.L. (2012) Kumar & Clark's clinical medicine (8th edition), Elsevier Saunders, Edinburgh, pp. 587-588.
  23. Lassi, Z.S., Haider, B.A. and Bhutta, Z.A. (2010) Zinc supplementation for the prevention of pneumonia in children aged 2 months to 59 months. Cochrane Database Syst. Rev., (12), CD005978.
  24. Suzuki, H., Asakawa, A., Li, J.B., Tsai, M., Amitani, H., Ohinata, K., Komai, M. and Inui, A. (2011) Zinc as an appetite stimulator-the possible role of zinc in the progression of diseases such as cachexia and sarcopenia. Recent Pat. Food Nutr. Agric., 3, 226-231. https://doi.org/10.2174/2212798411103030226
  25. Swardfager, W., Herrmann, N., Mazereeuw, G., Goldberger, K., Harimoto, T. and Lanctot, K.L. (2013) Zinc in depression: a meta-analysis. Biol. Psychiatry, 74, 872-878. https://doi.org/10.1016/j.biopsych.2013.05.008
  26. Muller, O. and Krawinkel, M. (2005) Malnutrition and health in developing countries. CMAJ, 173, 279-286. https://doi.org/10.1503/cmaj.050342
  27. World Health Organization and Food and Agriculture Organization of the United Nations (1994) Fats and oils in human nutrition: report of a joint expert consultation. FAO and WHO Expert Consultation, Rome, Available from: http://www.fao.org/docrep/V4700E/V4700E00.htm (Accessed Sep. 12, 2012).