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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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LC-PDA/MS/MS Analysis of Glucosinolates in Dolsan Leaf Mustard Kimchi and Dolsan Leaf Mustard Pickles

돌산갓 김치와 돌산갓 피클의 Glucosinolates의 LC-PDA/MS/MS분석

  • Oh, Sun Kyung (Department of Biomedical and Electronic Engineering, Chonnam National University) ;
  • Tsukamoto, Chigen (Department of Applied Biological Chemistry, Iwate University) ;
  • Kim, Ki Woong (Department of Marine Bio Food Science, Chonnam National University) ;
  • Choi, Myeong Rak (Department of Biomedical and Electronic Engineering, Chonnam National University)
  • 오선경 (전남대학교 바이오전자 메디컬공학 협동과정) ;
  • ;
  • 김기웅 (전남대학교 해양바이오 식품학과) ;
  • 최명락 (전남대학교 바이오전자 메디컬공학 협동과정)
  • Received : 2015.11.04
  • Accepted : 2016.02.09
  • Published : 2016.03.31
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Abstract

Changes in the concentrations of glucosinolates and related compounds in different extracts of Dolsan leaf mustard kimchi (DLMK) and Dolsan leaf mustard pickles (DLMP) were during storage investigated. Samples were kept at 0oC for 35 days and collected at 7 day intervals. The leaves and stems of DLMK and DLMP were refluxed for 24 h with 50% acetonitrile, and the extracts were analyzed by LC-PDA/MS/MS. The main glucosinolates detected in DLMK were sinigrin, gluconapoleiferin, glucobrassicanapin, and gluconapin, whereas those in DLMP were sinigrin, gluconapoleiferin, glucobrassicanapin, glucobrassicin, and glucoerucin. Sinigrin concentrations were quantified by UV absorption at 228 nm. Sinigrin concentrations in the leaves and stems of DLMK on the day of preparation were 2.14 mg/g and 2.25 mg/g, respectively, and those on day 35 after preparation were 1.25 mg/g and 1.00 mg/g, respectively. DLMP showed a similar trend: the concentrations in the leaves and stems on the day of preparation were 2.04 mg/g and 0.29 mg/g, respectively, whereas those on day 35 after preparation were 0.59 mg/g and 0.41 mg/g, respectively. Thus, sinigrin concentrations decreased during storage.

Keywords

References

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