Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Phylogenetic diversity and UV resistance analysis of radiation-resistant bacteria isolated from the water in Han River

한강물로부터 분리된 방사선 내성 세균들의 계통학적 다양성 및 UV 내성 분석

  • Lee, Jae-Jin (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Joo, Eun Sun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Lee, Do Hee (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Jung, Hee-Young (College of Agricultural and Life Sciences, Kyungpook National University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • 이재진 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 주은선 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 이도희 (서울여자대학교 자연과학대학 생명환경공학과) ;
  • 정희영 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김명겸 (서울여자대학교 자연과학대학 생명환경공학과)
  • Received : 2016.03.04
  • Accepted : 2016.03.22
  • Published : 2016.03.31
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Abstract

The aim of this study was to investigate the UV-resistance of radiation-resistant bacteria isolated from the water of Han River, South Korea. The water sample was irradiated with 3 kGy gamma radiation prior to isolation. Radiation-resistant bacterial strains were isolated by standard serial dilution method on R2A and 1/10 diluted R2A agar. The resulting purely isolated 60 cultures of bacteria were analysed for UV resistance and used in further studies. Based on the comparative analyses of 16S rRNA gene sequences, the bacterial isolates were divided into 3 phyla (4 genera): the phylum Deinococcus-Thermus (the genus Deinococcus) was 61.7%, Bacteroidetes (Hymenobacter and Spirosoma) was 23.4%, and Firmicutes (Exiguobacterium) was 15%. The results suggested that twenty-nine isolates are candidates new species belonging to Deinococcus, Hymenobacter, and Spirosoma, or other new genera. Nine bacterial strains were selected among the novel candidates and the UV-resistance analysis was conducted. All the candidate bacterial strains showed high UV resistance, similar to that of D. radiodurans R1.

이 논문은 서울 한강물에서 분리한 방사선 내성 세균군집과 분리된 신종 세균의 UV 내성 특성에 관한 내용이다. 세균은 R2A agar와 1/10 R2A agar를 사용하여 3 kGy가 조사된 한강물에서 분리되었다. 그 결과 방사선에 내성을 가지는 것으로 추측되는 균주를 60주 분리하였고, 본 연구 분석 자료로 사용하였다. 16S rRNA 유전자 염기서열 분석을 통해 분리균주 60개의 계통수를 파악한 결과, 3개의 문(4개의 속)이 확인되었고, Deinococcus-Thermus (Deinococcus)가 61.7%, Firmicutes(Exiguobacterium)는 15%, Bacteroidetes (Hymenobacter, Spirosoma)는 23.4%의 비중을 나타냈다. 분리균주 중 29개 균주가 Deinococcus, Hymenobacter, Spirosoma에 속하는 신종 또는 다른 신속으로 분류될 가능성을 보였으며, 앞으로 추가적인 신종 실험이 진행될 예정이다. 그리고 신종 예상균주를 9개 선정하여 UV 내성 실험을 진행한 결과, 9개 균주 모두 D. radiodurans $R1^T$ 균주 만큼 높은 UV 내성을 가지는 것으로 나타났다. 또한 분리된 Firmicutes (Exiguobacterium) 균주는 아직까지 방사선 내성 연구 보고가 되어 있지 않아서 추가적인 방사선 내성 연구가 필요하다.

Keywords

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