The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지
DOI QR코드

DOI QR Code

Disease Severity of Angular Leaf Spot Disease by Different Inoculation Method and Eco-Friendly Control Efficacy in Strawberry.

접종방법에 따른 딸기모무늬병원균의 발병도 검증 및 친환경 방제 효과

  • Kim, Da-Ran (Division of Applied Life Science, Gyeongsang National University) ;
  • Gang, Gun-hye (Division of Applied Life Science, Gyeongsang National University) ;
  • Cho, Hyun-ji (Division of Applied Life Science, Gyeongsang National University) ;
  • Yoon, Hae-Suk (Gyeongsangnam-do Agricultural Research and Extension Service) ;
  • Myoung, In Sik (National Academy of Agricultural Science) ;
  • kwak, Youn-Sig (Division of Applied Life Science, Gyeongsang National University)
  • Received : 2016.01.08
  • Accepted : 2016.03.25
  • Published : 2016.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, bacterial angular leaf spot disease, caused by Xanthomonas fragariae, causes severe damage in strawberry production and its' export to other countries, since the pathogen has been classified as an A2 quarantine pathogen. Typical the Angular Leaf Spot (ALS) disease represent that water-soaked angular spots symptoms, bacteria ooze exudate under relatively high humidity condition and later the spot become reddish brown on the leaf surface. The pathogen disseminated by irrigation water, infected mother plant and farmer's hand. In this study, we reported that rubbing inoculation method showed more effective in the pathogen dissemination than infiltration with needles, regardless the strawberry cultivars. Additionally, Dichloroisocyanurate (NaDCC) treatment in commercial strawberry fields provided reliable efficiency to reduce the bacterial angular leaf spot disease incidency and severity.

딸기 세균성모무늬병은 딸기를 기주로 하여 발생하며 큰 피해를 발생시킨다. 병원균의 전반은 관수의 형태, 감염된 모주의 이용 그리고 작업자에 의해 이루어지며 현재까지 명확한 화학적방제법이 개발되어 있지 않다. 2010년, 국내의 모무늬병원균에 대한 최초 보고에 따르면 병징으로서 잎 뒷면에 수침상의 각진 병반이 형성되며 습도가 높고 적합한 환경에서 세균침출액을 형성하게 되고 또한 진전된 경우 잎에서 적갈색의 병반이 형성된다고 보고하였다. 현재까지 이루어진 연구에 따르면 딸기 세균성모무늬병에 대하여 두 가지 품종이 저항성을 지니고 있다는 연구 결과가 있으며 감염된 포장에서는 수확량의 10%를 감소를 나타낸다고 알려져 있다. 그러나 이와 같은 모무늬병원균에 대한 국내에서의 활발한 연구가 이루어져 있지 않은 상황이다. 이에 본 연구는 딸기 모무늬병에 대하여 원인병원균이 Xanthomonas fragariae 병원균에 의하여 발생됨을 보고하는 바이며 병원성검증실험 진행한 결과 국내의 딸기재배 품종인 매향과 설향 중 매향이 감수성을 나타냄을 확인하였다. 또한 NaDCC처리시 98%이상의 방제가가 확인되었으므로 친환경방제제로서의 가능성을 제시하며 이러한 연구결과들은 국내에 발생되어있는 딸기 세균성모무늬병에 대하여 기초적 자료로서 보고하는 바이다.

Keywords

References

  1. Allan-Wojtas, R., P. D. Hildebrand, P. G. H. Braum, H. L. Smith-King, S. Carbyn and W. W. Renderos (2010) Low temperature and anhydrous electron microscopy techniques to observe the infection process of the bacterial pathogen Xanthomonas fragariae on strawberry leaves. J. Microscopy. 239:249-258. https://doi.org/10.1111/j.1365-2818.2010.03373.x
  2. CABI/EPPO: Xanthomonas fragariae. - Distribution maps of Quarantine Pests for Europe., 1998. Cab International, Wallingford, UK. No. 284.
  3. Fatima. F., I. Chaudhary, J. Ali, S. Rastogi and N. Pathak (2011) Microbial DNA extraction from soil by different methods and ITS PCR amplification. Biochem. Cellular Arch. 11: 85-90.
  4. Gillings, M. R., P. C. Fahy and J. Bradley (1998) Identification of Xanthomonas fragariae, the cause of an outbreak of angular leaf spot on strawberry in South Australia, and comparison with the cause of previous outbreaks in new south wales and New Zealand. Australasian Plant Pathol. 27:97-103. https://doi.org/10.1071/AP98012
  5. Jansea, J. D., M. P. Rossib, R. F. J. Gorkinka, J. H. J. Derksa, J. Swingsc, D. Janssensd and M. Scortichinib (2001) Bacterial leaf blight of strawberry (Fragaria (x) ananassa) caused by a pathovar of Xanthomonas arboricola, not similar to Xanthomonas fragariae Kennedy & King. Description of the causal organism as Xanthomonas arboricola pv. fragariae (pv. nov., comb. nov.). Plant Pathol 50:653-665. https://doi.org/10.1046/j.1365-3059.2001.00644.x
  6. Kim, D. -R., G. -H. Gang, H. -J. Cho, H. -S. Yoon and Y. -S. Kwak (2015) Evaluation of antimicrobial activity and disease control efficacy of sodium dichloroisocyanurate (NaDCC) against major strawberry diseases. Korean J. Pesticide Sci. 19:47-53. https://doi.org/10.7585/kjps.2015.19.1.47
  7. Kim, S. -K., C. Kim, J. Yoon and J. -H. Lee (2011) Inhibition of quorum sensing and biofilm formation by synthetic quorum signal analogues in Pseudomonas aeruginosa. Korean J. Microbiol. Biotech. 39:29-36.
  8. Kennedy, B. W. and T. H. King (1962) Angular leaf spot of strawberry caused by Xanthomonas fragariae sp. nov. Phytopathology 52:873-875.
  9. Kwon, J. -H., H. -S. Yoon, J. -S. Kim, C. -K. Shim and M. -H. Nam (2010) Angular leaf spot of strawberry caused by Xanthomonas fragariae. Plant Path. J. 16:97-100.
  10. Lewers, K. S., J. L. Maas, S. C. Hokanson, C. Gouin and J. S. Hartung (2003) Inheritance of resistance in strawberry to bacterial angular leafspot disease caused by Xanthomonas fragariae. J. Horticul Sci. 128:209-212.
  11. Mass, J. L. (1998) Compendium of strawberry diseases. 2nd ed APS press. 98pp.
  12. Nam, M. H., H. S. Kim, W. K. Lee, M. K. Gleason and H. G. Kim (2011) Control Efficacy of Gray Mold on Strawberry Fruits by Timing of Chemical and Microbial Fungicide Applications. Kor. J. Hort. Sci. Technol. 29:151-155.
  13. Perez-Jimenez, R. M., A. De Cal, P. Melgarejo, J. Cubero, C. Soria, T. Zea-Bonilla and I. Larena (2012) Resistance of several strawberry cultivars against three different pathogens. Spanish J. Agri. Res. 10:502-512. https://doi.org/10.5424/sjar/2012102-345-11
  14. Roberts, P. D., J. B. Jones, C. K. Chandler, R. E. Stall and R. D. Berger (1996) Survival of Xanthomonas fragariae on strawberry in summer nurseries in Florida detection by specific primers and nested polymerase chain reaction. Florida Agri. Experi. Station J. 80:1283-1288.
  15. Rowhani, A., A. J. Feliciano, T. Lips and W. D. Gubler (1994) Rapid identification of Xathomonas fragariae in infected strawberry leaves by enzyme-linked immunosorbent assay. Plant Dis. 78:248-250. https://doi.org/10.1094/PD-78-0248
  16. Yoon, H. S., J. Y. Kim, J. U. An, Y. H. Chang and K. P. Hong (2014) Effects of infected mother plants and irrigation methods on control of bacterial angular spot of strawberry. Korean J. Horticul. Sci. Technol. 32:80.
  17. Xue, S and R. H. Bors (2005) Resistance sources to Xanthomonas fragariae in non-octoploid strawberry species. Hori. Sci. 40: 1653-1656.
  18. Zimmermann, C., J. Hinrichs-Berger, E. Moltmann and H. Buchenauer (2004) Nested PCR (polymerase chain reaction) for detection of Xanthomonas fragariae in symptomless strawberry plants. J. Plant Dis. Prot. 111:39-51. https://doi.org/10.1007/BF03356131