Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Prevalence of Porphyromonas gingivalis fimA genotypes in peri-implant sulcus of Koreans using new primer

  • Kim, Sung-Geun (Department of Periodontology, Kyung Hee University School of Dentistry) ;
  • Hong, Ji-Youn (Department of Periodontology, Kyung Hee University School of Dentistry) ;
  • Shin, Seung-Il (Department of Periodontology, Kyung Hee University School of Dentistry) ;
  • Moon, Ji-Hoi (Department of Maxillofacial Biomedical Engineering, Kyung Hee University School of Dentistry) ;
  • Lee, Jin-Yong (Department of Maxillofacial Biomedical Engineering, Kyung Hee University School of Dentistry) ;
  • Herr, Yeek (Department of Periodontology, Kyung Hee University School of Dentistry)
  • Received : 2015.11.19
  • Accepted : 2016.01.23
  • Published : 2016.02.15
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Abstract

Purpose: Porphyromonas gingivalis fimA is a virulence factor associated with periodontal diseases, but its role in the pathogenesis of peri-implantitis remains unclear. We aimed to evaluate the relationship between the condition of peri-implant tissue and the distribution of P. gingivalis fimA genotypes in Koreans using a new primer. Methods: A total of 248 plaque samples were taken from the peri-implant sulci of 184 subjects. The control group consisted of sound implants with a peri-implant probing depth (PD) of 5 mm or less with no bleeding on probing (BOP). Test group I consisted of implants with a peri-implant PD of 5 mm or less and BOP, and test group II consisted of implants with a peri-implant PD of more than 5 mm and BOP. DNA was extracted from each sample and analyzed a using a polymerase chain reaction (PCR) with P. gingivalis -specific primers, followed by an additional PCR assay to differentiate the fimA genotypes in P. gingivalis-positive subjects. Results: The Prevalence of P. gingivalis in each group did not significantly differ (P>0.05). The most predominant fimA genotype in all groups was type II. The prevalence of type Ib fimA was significantly greater in test group II than in the control group (P<0.05). Conclusions: The fimA type Ib genotype of P. gingivalis was found to play a critical role in the destruction of peri-implant tissue, suggesting that it may be a distinct risk factor for periimplantitis.

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