Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Analysis and Anti-inflammatory of Lyoniside from the Pourthiaea villosa var. brunnea

떡윤노리나무로부터 분리된 Lyoniside의 함량분석과 항염증 효과

  • Woo, Kyeong Wan (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Seong, Tae Kyoung (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Lee, Hye Mi (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Jang, Ji Hun (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Lee, Ki Ho (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Cho, Hyun Woo (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Cho, Jung Hee (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • An, Byeongkwan (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine)
  • 우경완 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 성태경 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 이혜미 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 장지훈 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 이기호 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 조현우 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 조정희 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 안병관 (한약진흥재단 한약자원본부 한약재연구팀)
  • Received : 2015.11.27
  • Accepted : 2016.03.03
  • Published : 2016.03.31
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Abstract

In this study, the purification of the MeOH extract from the stems and leaves of Pourthiaea villosa var. brunnea using column chromatography furnished a main compound, lyoniside. The structure was elucidated on the basis of $^1H$ and $^{13}C$ NMR spectroscopic data. Quantitative analysis of lyoniside was conducted by HPLC method and the highest content of lyoniside was found in 50% MeOH reflux extraction. To investigate the anti-inflammatory effect of the lyoniside, we measured nitric oxide and pro-inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, IL-6 levels in lipopolysaccharide-induced murine macrophage cell line RAW 264.7. As a results, lyoniside decreased the level of nitric oxide and IL-6 in concentration dose dependent manner in RAW 264.7 cells.

Keywords

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