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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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ACE and α-glucosidase Inhibitory Activity of the Glucosinolates in Dolsan Leaf Mustard Pickle during Storage

저장기간 중 돌산갓피클에서 Glucosinolates의 ACE 및 α-glucosidase 저해활성

  • Son, Hae-Reon (Department of Biomedical and Electronic Engineering, Chonnam National University) ;
  • Oh, Sun-Kyung (Department of Biomedical and Electronic Engineering, Chonnam National University) ;
  • Tsukamoto, Chigen (Department of Applied Biological Chemistry, Iwate University) ;
  • Choi, Myeong-Rak (Department of Biomedical and Electronic Engineering, Chonnam National University)
  • 손혜련 (전남대학교 바이오전자메디컬협동과정) ;
  • 오선경 (전남대학교 바이오전자메디컬협동과정) ;
  • ;
  • 최명락 (전남대학교 바이오전자메디컬협동과정)
  • Received : 2016.06.14
  • Accepted : 2016.09.23
  • Published : 2016.09.30
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Abstract

This study was carried out for investigate that physiological activity, quantification and qualitative were sinigrin of Dolsan leaf mustard pickle (DLMP) during storage. sinigrin contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. ACE inhibitory activity was ranged between 43.2 and 79.4%. DLMP methanol extracts demonstrated highest ACE inhibitory activity at 79.4% on day 14, whereas DLMP ethanol extracts demonstrated highest Angiotensin I-converting Enzyme (ACE) inhibitory activity of 43.2% at day 0. The ${\alpha}-glucosidase$ inhibitory activity of positive control 0.02% (v/v) acarbose was 78%. The DLMP methanol extracts had the highest ${\alpha}-glucosidase$ inhibitory activity at 64.0% on day 14, whereas DLMP ethanol extracts had the lowest ${\alpha}-glucosidase$ inhibitory activity of 42.8% at day 28. Sinigrin was high in DLMP methanol extracts at $49.55{\mu}g/ml$ on day 14 of storage. Sinigrin standard was eluted at 2.73 min and MS analysis was m/z 283.03 along with fragment ions at m/z 204 and 149.06. These data show that sinigrin formed desulfo-glucosinolates $[M-SO_3-2H_2O+K+2H]^+$. Sinigrin concentration increased until day 14 and then decreased after that. DLMP methanol extracts had consistently higher sinigrin concentration than DLMP acetonitrile extracts during 28 days of storage.

Keywords

References

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