Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effect of Hydrosol Extracted from Chrysanthemum boreale Makino Flower on Proliferation and Migration in Human Skin Keratinocyte

산국화(Chrysanthemum boreale Makino) 유래 Hydrosol의 피부 각질형성세포 증식 및 이주 유도 활성에 미치는 효과

  • Kim, Do Yoon (Department of Cosmetic Science, College of Life and Health Science, Hoseo University) ;
  • Hwang, Dae Il (Department of Cosmetic Science, College of Life and Health Science, Hoseo University) ;
  • Yoon, Mi-so (Department of Cosmetic Science, College of Life and Health Science, Hoseo University) ;
  • Choi, In Ho (Department of Cosmetic Science, College of Life and Health Science, Hoseo University) ;
  • Lee, Hwan Myung (Department of Cosmetic Science, College of Life and Health Science, Hoseo University)
  • Received : 2016.01.23
  • Accepted : 2016.03.17
  • Published : 2016.03.30
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Abstract

In the present study, we extracted the hydrosol from flower of Chrysanthemum boreale Makino (CBMF hydrosol) by steam distillation and tested the effect of the CBMF hydrosol on skin regeneration using normal human keratinocytes (HaCaTs). CBMF hydrosol induced proliferation as well as migration in HaCaTs in a dose-dependent manner. Treatment with $1{\mu}g/mL$ CBMF hydrosol increased proliferation to $143.71{\pm}3.37%$ and migration to $139.98{\pm}5.72%$ compared with a control group. CBMF hydrosol also significantly enhanced the phosphorylations of extracellular signal-regulated kinase (Erk) 1/2 and serine/threonine-specific protein kinase (Akt) in HaCaTs. Moreover, CBMF hydrosol dose-dependently induced sprout outgrowth in HaCaTs. These results demonstrate that CBMF hydrosol has skin regeneration and wound healing activity in HaCaTs. Therefore, CBMF hydrosol could be used as a potential cosmetic material.

본 연구는 수증기 증류법을 이용하여 추출한 산국화 유래 hydrosol의 피부각질형성세포(HaCaTs) 증식 및 이주 유도 활성 평가를 통해 피부재상피화 및 상처치유 활성을 확인하였다. 산국화 hydrosol은 HaCaTs의 증식과 이주를 농도 의존적으로 유도하였으며, 특히 $1{\mu}g/mL$에서 음성 대조군(control)에 비해 $143.71{\pm}3.37%$의 증식과, $139.98{\pm}5.72%$의 이주 유도 활성을 나타내었다. 또한, 산국화 hydrosol은 extracellular signal-regulated kinase 1/2 (Erk 1/2)와 serine/threonine-specific protein kinase (Akt)의 인산화를 유의하게 증가시켰을 뿐만 아니라 collagen sprout out growth를 농도 의존적으로 유도하였다. 이러한 결과들을 통해서 산국화 hydrosol은 정상적인 재상피화 과정과 상처치유 등의 활성이 있음을 확인하였고, 향후 화장품 소재로서 응용가능성을 검증하였다.

Keywords

References

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