Journal of Dental Rehabilitation and Applied Science (구강회복응용과학지)

Korean Academy of Stomatognathic Function and Occlusion (대한턱관절교합학회)
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Effect of fluoride-containing gel on the roughness of a titanium surface and the promotion of bacterial growth

불소함유 겔이 티타늄 표면의 세균성 바이오필름 성장에 미치는 영향

  • Kim, Sun-Jin (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Jae-Kwan (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Chang, Beom-Seok (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Si-Young (Department of Oral Microbiology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Um, Heung-Sik (Department of Periodontology, Research Institute for Oral Sciences, College of Dentistry, Gangneung-Wonju National University)
  • 김선진 (강릉원주대학교 치과대학 치주과학교실) ;
  • 이재관 (강릉원주대학교 치과대학 치주과학교실) ;
  • 장범석 (강릉원주대학교 치과대학 치주과학교실) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학교실) ;
  • 엄흥식 (강릉원주대학교 치과대학 치주과학교실)
  • Received : 2015.09.21
  • Accepted : 2016.03.18
  • Published : 2016.03.31
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Abstract

Purpose: The aim of this study was to evaluate whether fluorides at various pH cause changes in the surface roughness of titanium implants that alter the adherence of bacterial biofilms. Materials and Methods: The titanium disks were assigned randomly to the following seven groups according to the fluoride agents and application time (1 minute or 30 minute) used: control (no treatment); group 1 (1.23% acidulated phosphate fluoride [APF] at pH 3.5 for 1 minute); group 2 (1.23% APF at pH 3.5 for 30 minute); group 3 (1.23% APF at pH 4.0 for 1 minute); group 4 (1.23% APF at pH 4.0 for 30 minute); group 5 (2% NaF gel at pH 7.0 for 1 minute); group 6 (2% NaF gel at pH 7.0 for 30 minute). The surface roughness of the titanium disks and the amount of adherent bacteria were measured. Results: Group 2 showed a significantly greater surface roughness than the control group (P < 0.0001). No significant differences in the amount of surface bacteria were observed between the treated samples and the controls. In addition, there were no significant differences in bacterial adherence relative to the incubation period between the treated samples and the controls. Conclusion: The surface roughness of the titanium disks was significantly greater after treatment with APF at pH 3.5 for 30 min compared with that of the controls. In addition, we found that the amount of Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibactor actinomycetemcomitans was similar among all groups

목적: 이번 연구는 다양한 pH의 불소 제재들이 티타늄 표면 거칠기에 미치는 영향을 평가하는 것이었다. 연구 재료 및 방법: 기계절삭형 티타늄 디스크를 시중에서 유통되는 세 가지 불소겔로 처리하였다. 불소겔의 종류와 처리 시간에 따라, 대조군, 1군(pH 3.5의 APF로 1분간 처리), 2군(pH 3.5의 APF로 30분간 처리), 3군(pH 4.0의 APF로 1분간 처리), 4군(pH 4.0의 APF로 30분간 처리), 5군(pH 7.0의 NaF로 1분간 처리), 6군(pH 7.0의 NaF로 30분간 처리)의 7군으로 분류하였다. 디스크의 표면 거칠기를 측정한 후, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum을 배양하여 디스크에 부착하는 세균의 양을 측정하였다. 결과: 표면 거칠기는 그룹2에서만 유의하게 증가하였다(P < 0.0001). 세균의 부착량은 실험군과 대조군 사이에 유의한 차이를 보이지 않았다. 결론: pH 3.5의 APF를 30분간 처리한 그룹에서 표면 거칠기가 유의하게 증가하였지만, 세균의 부착에 대해서는 유의한 차이를 보이지 않았다.

Keywords

References

  1. Adell R, Lekholm U, Rockler B, Branemark PI. A 15-year study of osseointegrated implants in the treatment of the edentulous jaw. Int J Oral Surg 1981;10:387-416. https://doi.org/10.1016/S0300-9785(81)80077-4
  2. Buser D, Mericske-Stern R, Bernard JP, Behneke A, Behneke N, Hirt HP, Belser UC, Lang NP. Longterm evaluation of non-submerged ITI implants. Part 1: 8-year life table analysis of a prospective multi-center study with 2359 implants. Clin Oral Implants Res 1997;8:161-72. https://doi.org/10.1034/j.1600-0501.1997.080302.x
  3. Romeo E, Lops D, Margutti E, Ghisolfi M, Chiapasco M, Vogel G. Long-term survival and success of oral implants in the treatment of full and partial arches: a 7-year prospective study with the ITI dental implant system. Int J Oral Maxillofac Implants 2004;19:247-59.
  4. Zitzmann NU, Berglundh T. Definition and prevalence of peri-implant diseases. J Clin Periodontol 2008;35:286-91. https://doi.org/10.1111/j.1600-051X.2008.01274.x
  5. Heitz-Mayfield LJ. Peri-implant diseases: diagnosis and risk indicators. J Clin Periodontol 2008;35:292-304. https://doi.org/10.1111/j.1600-051X.2008.01275.x
  6. Quirynen M, Listgarten MA. Distribution of bacterial morphotypes around natural teeth and titanium implants ad modum Branemark. Clin Oral Implants Res 1990;1:8-12. https://doi.org/10.1034/j.1600-0501.1990.010102.x
  7. Pontoriero R, Tonelli MP, Carnevale G, Mombelli A, Nyman SR, Lang NP. Experimentally induced periimplant mucositis. A clinical study in humans. Clin Oral Implants Res 1994;5:254-9. https://doi.org/10.1034/j.1600-0501.1994.050409.x
  8. Leonhardt A, Berglundh T, Ericsson I, Dahlen G. Putative periodontal pathogens on titanium implants and teeth in experimental gingivitis and periodontitis in beagle dogs. Clin Oral Implants Res 1992;3:112-9. https://doi.org/10.1034/j.1600-0501.1992.030303.x
  9. Leonhardt A, Renvert S, Dahlen G. Microbial findings at failing implants. Clin Oral Implants Res 1999;10:339-45. https://doi.org/10.1034/j.1600-0501.1999.100501.x
  10. Quirynen M, Marechal M, Busscher HJ, Weerkamp AH, Darius PL, van Steenberghe D. The influence of surface free energy and surface roughness on early plaque formation. An in vivo study in man. J Clin Periodontol 1990;17:138-44. https://doi.org/10.1111/j.1600-051X.1990.tb01077.x
  11. Bollen CM, Papaioanno W, Van Eldere J, Schepers E, Quirynen M, van Steenberghe D. The influence of abutment surface roughness on plaque accumulation and peri-implant mucositis. Clin Oral Implants Res 1996;7:201-11. https://doi.org/10.1034/j.1600-0501.1996.070302.x
  12. Teughels W, Van Assche N, Sliepen I, Quirynen M. Effect of material characteristics and/or surface topography on biofilm development. Clin Oral Implants Res 2006;17 Suppl 2:68-81. https://doi.org/10.1111/j.1600-0501.2006.01353.x
  13. Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol 1995;22:1-14.
  14. Bollen CM, Lambrechts P, Quirynen M. Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dent Mater 1997;13:258-69. https://doi.org/10.1016/S0109-5641(97)80038-3
  15. Aykent F, Yondem I, Ozyesil AG, Gunal SK, Avunduk MC, Ozkan S. Effect of different finishing techniques for restorative materials on surface roughness and bacterial adhesion. J Prosthet Dent 2010;103:221-7. https://doi.org/10.1016/S0022-3913(10)60034-0
  16. Dionysopoulos P, Gerasimou P, Tolidis K. The effect of home-use fluoride gels on glass-ionomer, compomer and composite resin restorations. J Oral Rehabil 2003;30:683-9. https://doi.org/10.1046/j.1365-2842.2003.01104.x
  17. Benderli Y, Gokce K, Kazak M. Effect of APF gel on micromorphology of resin modified glassionomer cements and flowable compomers. J Oral Rehabil 2005;32:669-75. https://doi.org/10.1111/j.1365-2842.2005.01484.x
  18. Walker MP, Ries D, Kula K, Ellis M, Fricke B. Mechanical properties and surface characterization of beta titanium and stainless steel orthodontic wire following topical fluoride treatment. Angle Orthod 2007;77:342-8. https://doi.org/10.2319/0003-3219(2007)077[0342:MPASCO]2.0.CO;2
  19. Khoury ES, Abboud M, Bassil-Nassif N, Bouserhal J. Effect of a two-year fluoride decay protection protocol on titanium brackets. Int Orthod 2011;9:432-51.
  20. Stajer A, Urban E, Pelsoczi IK, Mihalik E, Rakonczay Z, Nagy K, Turzo K, Radnai M. Effect of caries preventive products on the growth of bacterial biofilm on titanium surface. Acta Microbiol Immunol Hung 2012;59:51-61. https://doi.org/10.1556/AMicr.59.2012.1.6
  21. Probster L, Lin W, Huttemann H. Effect of fluoride prophylactic agents on titanium surfaces. Int J Oral Maxillofac Implants 1992;7:390-4.
  22. Hix JO, O'Leary TJ. The relationship between cemental caries, oral hygiene status and fermentable carbohydrate intake. J Periodontol 1976;47:398-404. https://doi.org/10.1902/jop.1976.47.7.398
  23. Ravald N, Hamp SE. Prediction of root surface caries in patients treated for advanced periodontal disease. J Clin Periodontol 1981;8:400-14. https://doi.org/10.1111/j.1600-051X.1981.tb00889.x
  24. Cobb HB, Rozier RG, Bawden JW. A clinical study of the caries preventive effects of an APF solution and APF thixotropic gel. Pediatr Dent 1980;2:263-6.
  25. Horowitz HS, Doyle J. The effect on dental caries of topically applied acidulated phosphatefluoride: results after three years. J Am Dent Assoc 1971;82:359-65. https://doi.org/10.14219/jada.archive.1971.0063
  26. Reclaru L, Meyer JM. Effects of fluorides on titanium and other dental alloys in dentistry. Biomaterials 1998;19:85-92. https://doi.org/10.1016/S0142-9612(97)00179-8
  27. Schiff N, Grosgogeat B, Lissac M, Dalard F. Influence of fluoride content and pH on the corrosion resistance of titanium and its alloys. Biomaterials 2002;23:1995-2002. https://doi.org/10.1016/S0142-9612(01)00328-3
  28. Toniollo MB, Tiossi R, Macedo AP, Rodrigues RC, Ribeiro RF, Mattos Mda G. Effect of fluoridecontaining solutions on the surface of cast commercially pure titanium. Braz Dent J 2009;20:201-4. https://doi.org/10.1590/S0103-64402009000300005
  29. Amoroso PF, Adams RJ, Waters MG, Williams DW. Titanium surface modification and its effect on the adherence of Porphyromonas gingivalis: an in vitro study. Clin Oral Implants Res 2006;17:633-7. https://doi.org/10.1111/j.1600-0501.2006.01274.x
  30. Kim YJ, Jang KT, Garcia-Godoy F. Effect of acidulated phosphate fluoride (APF) gel on the adherence of cariogenic bacteria to resin composites. Am J Dent 2005;18:91-4.