Parasites, Hosts and Diseases

  • 제54권1호
  • /
  • Pages.103-107
  • /
  • 2016
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Effects of Disinfectants on Larval Development of Ascaris suum Eggs

  • Oh, Ki-Seok (Department of Obstetrics, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Geon-Tae (Department of Parasitology, College of Veterinary Medicine, Chonnam National University) ;
  • Ahn, Kyu-Sung (Department of Parasitology, College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Sung-Shik (Department of Parasitology, College of Veterinary Medicine, Chonnam National University)
  • 투고 : 2015.12.17
  • 심사 : 2016.01.11
  • 발행 : 2016.02.29
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The objective of this study was to evaluate the effects of several different commercial disinfectants on the embryogenic development of Ascaris suum eggs. A 1-ml aliquot of each disinfectant was mixed with approximately 40,000 decorticated or intact A. suum eggs in sterile tubes. After each treatment time (at 0.5, 1, 5, 10, 30, and 60 min), disinfectants were washed away, and egg suspensions were incubated at $25^{\circ}C$ in distilled water for development of larvae inside. At 3 weeks of incubation after exposure, ethanol, methanol, and chlorohexidin treatments did not affect the larval development of A. suum eggs, regardless of their concentration and treatment time. Among disinfectants tested in this study, 3% cresol, 0.2% sodium hypochlorite and 0.02% sodium hypochlorite delayed but not inactivated the embryonation of decorticated eggs at 3 weeks of incubation, because at 6 weeks of incubation, undeveloped eggs completed embryonation regardless of exposure time, except for 10% povidone iodine. When the albumin layer of A. suum eggs remained intact, however, even the 10% povidone iodine solution took at least 5 min to reasonably inactivate most eggs, but never completely kill them with even 60 min of exposure. This study demonstrated that the treatment of A. suum eggs with many commercially available disinfectants does not affect the embryonation. Although some disinfectants may delay or stop the embryonation of A. suum eggs, they can hardly kill them completely.

키워드

참고문헌

  1. Overgaauw PA, van Zutphen L, Hoek D, Yaya FO, Roelfsema J, Pinelli E, van Knapen F, Kortbeek LM. Zoonotic parasites in fecal samples and fur from dogs and cats in The Netherlands. Vet Parasitol 2009; 163: 115-122. https://doi.org/10.1016/j.vetpar.2009.03.044
  2. Leles D, Gardner SL, Reinhard K, Iniguez A, Araujo A. Are Ascaris lumbricoides and Ascaris suum a single species? Parasit Vectors 2012; 5: 42. https://doi.org/10.1186/1756-3305-5-42
  3. Woodruff A, De Savigny D, Jacobs D. Study of toxocaral infection in dog breeders. Br Med J 1978; 2: 1747-1748.
  4. Robertson ID, Thompson R. Enteric parasitic zoonoses of domesticated dogs and cats. Microbes Infect 2002; 4: 867-873. https://doi.org/10.1016/S1286-4579(02)01607-6
  5. Shin SS. Parasitic diseases of companion animals. Hanyang Med Rev 2010; 30: 246-264. https://doi.org/10.7599/hmr.2010.30.3.246
  6. Overgaauw PA, van Knapen F. Veterinary and public health aspects of Toxocara spp. Vet Parasitol 2013; 193: 398-403. https://doi.org/10.1016/j.vetpar.2012.12.035
  7. Sinniah B. Daily egg production of Ascaris lumbricoides: the distribution of eggs in the faeces and the variability of egg counts. Parasitology 1982; 84: 167-175. https://doi.org/10.1017/S0031182000051763
  8. O'lorcain P, Holland C. The public health importance of Ascaris lumbricoides. Parasitology 2000; 121: S51-S71. https://doi.org/10.1017/S0031182000006442
  9. Barrett J. Studies on the induction of permeability in Ascaris lumbricoides eggs. Parasitology 1976; 73: 109-121. https://doi.org/10.1017/S0031182000051374
  10. Brownell SA, Nelson KL. Inactivation of single-celled Ascaris suum eggs by low-pressure UV radiation. Appl Environ Microbiol 2006; 72: 2178-2184. https://doi.org/10.1128/AEM.72.3.2178-2184.2006
  11. Butkus MA, Hughes KT, Bowman DD, Liotta JL, Jenkins MB, Labare MP. Inactivation of Ascaris suum by short-chain fatty acids. Appl Environ Microbiol 2011; 77: 363-366. https://doi.org/10.1128/AEM.01675-10
  12. Rosypal AC, Bowman DD, Holliman D, Flick GJ, Lindsay DS. Effects of high hydrostatic pressure on embryonation of Ascaris suum eggs. Vet Parasitol 2007; 145: 86-89. https://doi.org/10.1016/j.vetpar.2006.11.001
  13. Pecson BM, Nelson KL. Inactivation of Ascaris suum eggs by ammonia. Environ Sci Technol 2005; 39: 7909-7914. https://doi.org/10.1021/es050659a
  14. Zaman V, Visuvalingam N. Action of aqueous iodine on ova of Ascaris lumbricoides and Ascaris suum. Trans R Soc Trop Med Hyg 1967; 61: 443-444. https://doi.org/10.1016/0035-9203(67)90026-0
  15. Labare MP, Soohoo H, Kim D, yan Tsoi K, Liotta JL, Bowman DD. Ineffectiveness of a quaternary ammonium salt and povidone-iodine for the inactivation of Ascaris suum eggs. Am J Infect Control 2013; 41: 360-361. https://doi.org/10.1016/j.ajic.2012.05.013
  16. Wattal C, Malla N, Khan I, Agarwal S. Reversible inhibition of development and hatching of infective eggs of Ascaris lumbricoides var. hominis. J Parasitol 1985; 71: 518. https://doi.org/10.2307/3281551
  17. Feachem R, Bradley D, Garelick H, Mara D. Ascaris and ascariasis. Sanitation and disease: health aspects of excreta and wastewater management. Washington DC, USA. World Bank. 1983, pp. 391.
  18. Borgsteede F. Effects of various disinfectants on the development and survival possibilities of the pre-parasitic stages of Ostertagia ostertagi, Cooperia oncophora and Ascaris suum. Tijdschr Diergeneesk 1987; 112: 769-778.
  19. Zamora JL. Chemical and microbiologic characteristics and toxicity of povidone-iodine solutions. Am J Surg 1986; 151: 400-406. https://doi.org/10.1016/0002-9610(86)90477-0
  20. Lindquist TD, Maxwell AJ, Miller TD, Win'E TL, Novicki T, Fritsche TR, Iliakis B, Montoya M. Preparation of corneal donor eyes comparing 1% versus 5% povidone iodine. Cornea 2011; 30: 333-337. https://doi.org/10.1097/ICO.0b013e3181eeb5d2
  21. Pelletier JS, Miller D, Liang B, Capriotti JA. In vitro efficacy of a povidone-iodine 0.4% and dexamethasone 0.1% suspension against ocular pathogens. J Cataract Refract Surg 2011; 37: 763-766. https://doi.org/10.1016/j.jcrs.2010.11.028
  22. Ito H, Ito T, Hikida M, Yashiro J, Otsuka A, Kida H, Otsuki K. Outbreak of highly pathogenic avian influenza in Japan and anti-influenza virus activity of povidone-iodine products. Dermatology 2006; 212: 115-118. https://doi.org/10.1159/000089210
  23. Morrondo P, Diez-Morrondo C, Pedreira J, Diez-Banos N, Senchez-Andrade R, Paz-Silva A, Diez-Banos P. Toxocara canis larvae viability after disinfectant-exposition. Parasitol Res 2006; 99: 558-561. https://doi.org/10.1007/s00436-006-0200-5
  24. Verocai G, Tavares P, Ribeiro DA, Correia T, Scott F. Effects of disinfectants on Toxocara canis embryogenesis and larval establishment in mice tissues. Zoonoses Public Health 2010; 57: e213-e216. https://doi.org/10.1111/j.1863-2378.2010.01330.x
  25. Krishnaswami S, Post F. Effect of chlorine on Ascaris (Nematoda) eggs. Health Lab Sci 1968; 5: 225.

피인용 문헌

  1. Effects of In Vivo and In Vitro Treatment of Ascaris suum Eggs with Anthelmintic Agents on Embryonation and Infectivity for Mice vol.103, pp.5, 2016, https://doi.org/10.1645/17-21
  2. In vitro study of disinfectants on the embryonation and survival of Toxascaris leonina eggs vol.92, pp.5, 2016, https://doi.org/10.1017/s0022149x17000839
  3. Membrane-separated electrochemical latrine wastewater treatment vol.5, pp.1, 2019, https://doi.org/10.1039/c8ew00698a
  4. Assessment of the inhibitory effects of disinfectants on the embryonation of Ascaridia columbae eggs vol.14, pp.5, 2019, https://doi.org/10.1371/journal.pone.0217551
  5. Effects of single and integrated water, sanitation, handwashing, and nutrition interventions on child soil-transmitted helminth and Giardia infections: A cluster-randomized controlled trial in rural K vol.16, pp.6, 2016, https://doi.org/10.1371/journal.pmed.1002841
  6. Viability of Pseudocapillaria tomentosa Eggs Exposed to Heat, Ultraviolet Light, Chlorine, Iodine, and Desiccation vol.16, pp.5, 2016, https://doi.org/10.1089/zeb.2019.1736
  7. Is routine disinfection efficient in preventing contamination with Toxocara canis eggs? vol.94, pp.None, 2016, https://doi.org/10.1017/s0022149x1900052x
  8. The impact of certain flavourings and preservatives on the survivability of eggs of Ascaris suum and Trichuris suis vol.11, pp.2, 2016, https://doi.org/10.15421/022052
  9. Deactivation of Ascaris suum eggs using electroporation and sequential inactivation with chemical disinfection vol.10, pp.3, 2016, https://doi.org/10.2166/washdev.2020.192
  10. Efficacy of a chlorocresol-based disinfectant product on Toxocara canis eggs vol.119, pp.10, 2016, https://doi.org/10.1007/s00436-020-06769-2
  11. Inactivating Effects of Common Laboratory Disinfectants, Fixatives, and Temperatures on the Eggs of Soil Transmitted Helminths vol.9, pp.3, 2021, https://doi.org/10.1128/spectrum.01828-21