대한수의학회지 (Korean Journal of Veterinary Research)

  • 제56권1호
  • /
  • Pages.23-28
  • /
  • 2016
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
DOI QR코드

DOI QR Code

최근 국내에서 유행하는 돼지 유행성 설사 바이러스 nucleocapsid 유전자의 다양성 및 계통 분석

Genetic diversity of nucleocapsid genes of recent porcine epidemic diarrhea viruses isolated in Korea

  • 김기주 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 박유경 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 박보경 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 람쯩꽝 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 박소연 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 김재훈 (제주대학교 수의과대학) ;
  • 한태욱 (강원대학교 수의과대학 및 동물의학종합연구소)
  • Kim, Kiju (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Park, Yookyung (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Park, Bokyung (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Truong, Quang Lam (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Park, Soyeon (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Jaehun (College of Veterinary Medicine, Jeju National University) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2016.01.14
  • 심사 : 2016.03.02
  • 발행 : 2016.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Porcine epidemic diarrhea virus (PEDV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea in piglets, resulting in large economic losses because of high mortality. In November 2013, PEDV reemerged in Korea, and these outbreaks have since continuously occurred. In the present study, we determined the full-length nucleocapsid (N) gene sequences of three Korean PEDV field isolates collected in 2014-2015. Sequence and phylogenetic analysis of N genes revealed that recent prevalent Korean PEDV isolates were very closely related to the US PEDV isolates in 2013. Interestingly, the phylogenetic tree based on the nucleotide sequencing of the PEDV N gene was similar to the tree topology of the PEDV complete genomes. Therefore, our data provide a better understanding of the genetic diversity and contribute to the accurate diagnosis and development of vaccines against PEDV.

키워드

참고문헌

  1. Chen JF, Sun DB, Wang CB, Shi HY, Cui XC, Liu SW, Qiu HJ, Feng L. Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China. Virus Genes 2008, 36, 355-364. https://doi.org/10.1007/s11262-007-0196-7
  2. Choi JC, Lee KK, Pi JH, Park SY, Song CS, Choi IS, Lee JB, Lee DH, Lee SW. Comparative genome analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains isolated in Korea. Infect Genet Evol 2014, 26, 348-351. https://doi.org/10.1016/j.meegid.2014.06.005
  3. Debouck P, Pensaert M. Experimental infection of pigs with a new porcine enteric coronavirus, CV 777. Am J Vet Res 1980, 41, 219-223.
  4. Hoang H, Killian ML, Madson DM, Arruda PH, Sun D, Schwartz KJ, Yoon KJ. Full-length genome sequence of a plaque-cloned virulent porcine epidemic diarrhea virus isolate (USA/Iowa/18984/2013) from a Midwestern U.S. swine herd. Genome Announc 2013, 1, e01049-13.
  5. Hou XL, Yu LY, Liu J. Development and evaluation of enzyme-linked immunosorbent assay based on recombinant nucleocapsid protein for detection of porcine epidemic diarrhea (PEDV) antibodies. Vet Microbiol 2007, 123, 86-92. https://doi.org/10.1016/j.vetmic.2007.02.014
  6. Huang YW, Dickerman AW, Pineyro P, Li L, Fang L, Kiehne R, Opriessnig T, Meng XJ. Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States. MBio 2013, 4, e00737-13.
  7. Kim SH, Lee JM, Jung J, Kim IJ, Hyun BH, Kim HI, Park CK, Oem JK, Kim YH, Lee MH, L KK. Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013. Arch Virol 2015, 160, 1055-1064. https://doi.org/10.1007/s00705-015-2353-y
  8. Kim SY, Song DS, Park BK. Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR. J Vet Diagn Invest 2001, 13, 516-520. https://doi.org/10.1177/104063870101300611
  9. Kim Y, Lee C. Porcine epidemic diarrhea virus induces caspase-independent apoptosis through activation of mitochondrial apoptosis-inducing factor. Virology 2014, 460-461, 180-193. https://doi.org/10.1016/j.virol.2014.04.040
  10. Kocherhans R, Bridgen A, Ackermann M, Tobler K. Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence. Virus Genes 2001, 23, 137-144. https://doi.org/10.1023/A:1011831902219
  11. Kubota S, Sasaki O, Amimoto K, Okada N, Kitazima T, Yasuhara H. Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus. J Vet Med Sci 1999, 61, 827-830. https://doi.org/10.1292/jvms.61.827
  12. Kweon C, Kwon B, Jung T, Kee Y, Hur D, Hwang E, Rhee J, An S. Isolation of porcine epidemic diarrhea virus (PEDV) in Korea. Korean J Vet Res 1993, 33, 249-254.
  13. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG. Clustal W and Clustal X version 2.0. Bioinformatics 2007, 23, 2947-2948. https://doi.org/10.1093/bioinformatics/btm404
  14. Lee HK, Yeo SG. Cloning and sequence analysis of the nucleocapsid gene of porcine epidemic diarrhea virus Chinju99. Virus Genes 2003, 26, 207-212. https://doi.org/10.1023/A:1023447732567
  15. Lee S, Kim Y, Lee C. Isolation and characterization of a Korean porcine epidemic diarrhea virus strain KNU-141112. Virus Res 2015, 208, 215-224. https://doi.org/10.1016/j.virusres.2015.07.010
  16. Lee S, Ko DH, Kwak SK, Lim CH, Moon SU, Lee DS, Lee C. Reemergence of porcine epidemic diarrhea virus on Jeju Island. Korean J Vet Res 2014, 54, 185-188. https://doi.org/10.14405/kjvr.2014.54.3.185
  17. Lee S, Lee C. Outbreak-related porcine epidemic diarrhea virus strains similar to US strains, South Korea, 2013. Emerg Infect Dis 2014, 20, 1223-1226. https://doi.org/10.3201/eid2007.140294
  18. Lee S, Park GS, Shin JH, Lee C. Full-genome sequence analysis of a variant strain of porcine epidemic diarrhea virus in South Korea. Genome Announc 2014, 2, e01116-14.
  19. Liu DQ, Ge JW, Qiao XY, Jiang YP, Liu SM, Li YJ. High-level mucosal and systemic immune responses induced by oral administration with Lactobacillus-expressed porcine epidemic diarrhea virus (PEDV) S1 region combined with Lactobacillus-expressed N protein. Appl Microbiol Biotechnol 2012, 93, 2437-2446. https://doi.org/10.1007/s00253-011-3734-0
  20. Li Z, Chen F, Yuan Y, Zeng X, Wei Z, Zhu L, Sun B, Xie Q, Cao Y, Xue C, Ma J, Bee Y. Sequence and phylogenetic analysis of nucleocapsid genes of porcine epidemic diarrhea virus (PEDV) strains in China. Arch Virol 2013, 158, 1267-1273. https://doi.org/10.1007/s00705-012-1592-4
  21. Oldham J. Letter to the editor. Pig Farming 1972, October (Suppl), 72-73.
  22. Park SJ, Kim HK, Song DS, An DJ, Park BK. Complete genome sequences of a Korean virulent porcine epidemic diarrhea virus and its attenuated counterpart. J Virol 2012, 86, 5964. https://doi.org/10.1128/JVI.00557-12
  23. Park SJ, Kim HK, Song DS, Moon HJ, Park BK. Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea. Arch Virol 2011, 156, 577-585. https://doi.org/10.1007/s00705-010-0892-9
  24. Pijpers A, van Nieuwstadt AP, Terpstra C, Verheijden JH. Porcine epidemic diarrhoea virus as a cause of persistent diarrhoea in a herd of breeding and finishing pigs. Vet Rec 1993, 132, 129-131. https://doi.org/10.1136/vr.132.6.129
  25. Saif LJ. Coronavirus immunogens. Vet Microbiol 1993, 37, 285-297. https://doi.org/10.1016/0378-1135(93)90030-B
  26. Sun R, Leng Z, Zhai SL, Chen D, Song C. Genetic variability and phylogeny of current Chinese porcine epidemic diarrhea virus strains based on spike, ORF3, and membrane genes. ScientificWorldJournal 2014, 2014, 208439.
  27. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24, 1596-1599. https://doi.org/10.1093/molbev/msm092

피인용 문헌

  1. Genetic sequence and phylogenetic analysis of spike genes of Porcine epidemic diarrhea virus (PEDV) in Chung-Nam area vol.39, pp.4, 2016, https://doi.org/10.7853/kjvs.2016.39.4.259
  2. Regulatory Effect of Methylation of the Porcine AQP3 Gene Promoter Region on Its Expression Level and Porcine Epidemic Diarrhea Virus Resistance vol.11, pp.10, 2020, https://doi.org/10.3390/genes11101167