Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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The hepatitis B virus X protein induced fibrosis in Huh7 cells

간암세포주 Huh7에서 Hepatitis B virus X protein에 의한 간섬유화

  • Son, Moa (Faculty of Biotechnology, College of Applied Life Sciences, SARI, Jeju National University) ;
  • Park, Sanggyu (Division of Life & Environmental Science, Daegu University) ;
  • Cho, Moonjae (Department of Biochemistry and Institute of Medical Science, School of Medicine, Jeju National University)
  • Received : 2015.10.23
  • Accepted : 2015.11.04
  • Published : 2016.03.31
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Abstract

Hepatitis B virus infection can cause hepatic fibrosis leading to cirrhosis and hepatocellular carcinoma. However the mechanism remains poorly understood. In this study, we found that Hepatitis B virus X-protein (HBx) increases vimentin, fibronectin, slug, snail and NOX4 expression. Because NOX4-mediated reactive oxygen species can increase slug and snail, which can induce fibrosis, HBx may be a key regulator of hepatic fibrosis development via NOX4 induction.

B형 간염 바이러스는 간경변증과 간세포암의 원인이 되는 간섬유화를 유발한다. 하지만 현재까지 그와 관련한 자세한 메커니즘은 밝혀지지 않았기에 본 연구에서 이를 알아보고자 하였다. 실험 결과, B형 간염 바이러스에서 발현되는 HBx 단백질에 의해 vimentin, fibronectin, slug, snail, NOX4가 증가되는 것을 확인하였다. NOX4에 의한 활성산소가 snail, slug 발현을 유도하고 섬유화 과정을 촉진할 수 있기 때문에 NOX4의 발현을 유도하는 HBx가 간섬유화를 조절, 유도하는 것을 확인하였다.

Keywords

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