Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Effects of Fasting on Brain Expression of Kiss2 and GnRH I and Plasma Levels of Sex Steroid Hormones, in Nile Tilapia Oreochromis niloticus

절식이 나일 틸라피아 Oreochromis niloticus의 Kiss2, GnRH I mRNA 발현 및 성 스테로이드 호르몬 농도에 미치는 영향

  • Park, Jin Woo (Marine Ecosystem and Biological Research Center, KIOST) ;
  • Kwon, Joon Yeong (Department of Aquatic Life Medical Sciences, College of Health Science, Sunmoon University) ;
  • Jin, Ye Hwa (Department of Aquatic Life Medical Sciences, College of Health Science, Sunmoon University) ;
  • Oh, Sung-Yong (Tongyeong Marine Science Station, KIOST)
  • 박진우 (한국해양과학기술원 생태기반연구센터) ;
  • 권준영 (선문대학교 건강보건대학 수산생명의학과) ;
  • 진예화 (선문대학교 건강보건대학 수산생명의학과) ;
  • 오승용 (한국해양과학기술원 통영해상과학기지)
  • Received : 2015.09.07
  • Accepted : 2016.02.25
  • Published : 2016.03.30
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Abstract

In many fish species, including Nile tilapia (Oreochromis niloticus), gonadal development occurs at the expense of stored energy and nutrients. Therefore, reproductive systems are inhibited by limited food supply. It has been well established that reproductive function is highly sensitive to both metabolic status and energy balance. Nothing is known about the possible mediated connection between energy balance and reproduction. Kisspeptin, a neuropeptide product of the Kiss gene has emerged as an essential gatekeeper of reproduction and may be possibly be linked to energy balance and reproduction in non-mammalians. Thus, in this study, the effect of fasting (10 days) on the expression of kisspeptin and the gonadotropin-releasing hormone (GnRH) gene were assessed in Nile tilapia (male and female) using qRT-PCR. In addition, plasma levels of estradiol-$17{\beta}$ ($E_2$) and 11-ketotestosterone (11-KT) in adult tilapia were measured by ELISA. In male tilapia, fasting reduced Kiss2 and GnRH I mRNA expression in the brain and 11-KT level in comparison with the fed tilapia (p < 0.05). In females, however, there were no significant differences in GnRH I mRNA expression and $E_2$ between fish subjected to fasting and those fed (p > 0.05). These data indicate the impact of nutritional states on kisspeptin as a potential regulatory mechanism for the control of reproduction in male Nile tilapia.

Keywords

References

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