Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Human Amnion-Derived Mesenchymal Stem Cells Protect Human Bone Marrow Mesenchymal Stem Cells against Oxidative Stress-Mediated Dysfunction via ERK1/2 MAPK Signaling

  • Wang, Yuli (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University) ;
  • Ma, Junchi (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University) ;
  • Du, Yifei (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University) ;
  • Miao, Jing (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University) ;
  • Chen, Ning (Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University)
  • Received : 2015.06.03
  • Accepted : 2015.11.23
  • Published : 2016.03.31
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Abstract

Epidemiological evidence suggests that bone is especially sensitive to oxidative stress, causing bone loss in the elderly. Previous studies indicated that human amnion-derived mesenchymal stem cells (HAMSCs), obtained from human amniotic membranes, exerted osteoprotective effects in vivo. However, the potential of HAMSCs as seed cells against oxidative stress-mediated dysfunction is unknown. In this study, we systemically investigated their antioxidative and osteogenic effects in vitro. Here, we demonstrated that HAMSCs significantly promoted the proliferation and osteoblastic differentiation of $H_2O_2$-induced human bone marrow mesenchymal stem cells (HBMSCs), and down-regulated the reactive oxygen species (ROS) level. Further, our results suggest that activation of the ERK1/2 MAPK signal transduction pathway is essential for both HAMSCs-mediated osteogenic and protective effects against oxidative stress-induced dysfunction in HBMSCs. U0126, a highly selective inhibitor of extracellular ERK1/2 MAPK signaling, significantly suppressed the antioxidative and osteogenic effects in HAMSCs. In conclusion, by modulating HBMSCs, HAMSCs show a strong potential in treating oxidative stress- mediated bone deficiency.

Keywords

References

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