DOI QR코드

DOI QR Code

Characterization of Caveola-Vesicle Complexes (CVCs) Protein, PHIST/CVC-8195 in Plasmodium vivax

  • Wang, Bo (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Lu, Feng (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Han, Jin-Hee (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Lee, Seong-Kyun (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Cheng, Yang (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Nyunt, Myat Htut (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University) ;
  • Ha, Kwon-Soo (Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University) ;
  • Hong, Seok-Ho (Department of Internal Medicine, School of Medicine, Kangwon National University) ;
  • Park, Won Sun (Department of Physiology, School of Medicine, Kangwon National University) ;
  • Han, Eun-Taek (Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University)
  • Received : 2016.08.23
  • Accepted : 2016.11.29
  • Published : 2016.12.31

Abstract

Plasmodium vivax produces numerous caveola-vesicle complex (CVC) structures beneath the membrane of infected erythrocytes. Recently, a member helical interspersed subtelomeric (PHIST) superfamily protein, $PcyPHIST/CVC-81_{95}$, was identified as CVCs-associated protein in Plasmodium cynomolgi and essential for survival of this parasite. Very little information has been documented to date about $PHIST/CVC-81_{95}$ protein in P. vivax. In this study, the recombinant $PvPHIST/CVC-81_{95}$ N and C termini were expressed, and immunoreactivity was assessed using confirmed vivax malaria patients sera by protein microarray. The subcellular localization of $PvPHIST/CVC-81_{95}$ N and C termini in blood stage parasites was also determined. The antigenicity of recombinant $PvPHIST/CVC-81_{95}$ N and C terminal proteins were analyzed by using serum samples from the Republic of Korea. The results showed that immunoreactivities to these proteins had 61% and 43% sensitivity and 96.9% and 93.8% specificity, respectively. The N terminal of $PvPHIST/CVC-81_{95}$ which contains transmembrane domain and export motif (PEXEL; RxLxE/Q/D) produced CVCs location throughout the erythrocytic-stage parasites. However, no fluorescence was detected with antibodies against C terminal fragment of $PvPHIST/CVC-81_{95}$. These results suggest that the $PvPHIST/CVC-81_{95}$ is localized on the CVCs and may be immunogenic in natural infection of P. vivax.

Keywords

References

  1. Murray CJ, Rosenfeld LC, Lim SS, Andrews KG, Foreman KJ, Haring D, Fullman N, Naghavi M, Lozano R, Lopez AD. Global malaria mortality between 1980 and 2010: a systematic analysis. Lancet 2012; 379: 413-431. https://doi.org/10.1016/S0140-6736(12)60034-8
  2. Collins WE. Plasmodium knowlesi: a malaria parasite of monkeys and humans. Annu Rev Entomol 2012; 57: 107-121. https://doi.org/10.1146/annurev-ento-121510-133540
  3. Miller LH, Ackerman HC, Su XZ, Wellems TE. Malaria biology and disease pathogenesis: insights for new treatments. Nat Med 2013; 19: 156-167. https://doi.org/10.1038/nm.3073
  4. Mueller I, Galinski MR, Tsuboi T, Arevalo-Herrera M, Collins WE, King CL. Natural acquisition of immunity to Plasmodium vivax: epidemiological observations and potential targets. Adv Parasitol 2013; 81: 77-131.
  5. Kilejian A. Characterization of a protein correlated with the production of knob-like protrusions on membranes of erythrocytes infected with Plasmodium falciparum. Proc Natl Acad Sci USA 1979; 76: 4650-4653. https://doi.org/10.1073/pnas.76.9.4650
  6. McMillan PJ, Millet C, Batinovic S, Maiorca M, Hanssen E, Kenny S, Muhle RA, Melcher M, Fidock DA, Smith JD, Dixon MW, Tilley L. Spatial and temporal mapping of the PfEMP1 export pathway in Plasmodium falciparum. Cell Microbiol 2013; 15: 1401-1418. https://doi.org/10.1111/cmi.12125
  7. Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. Rosetting Plasmodium falciparum-infected erythrocytes bind to human brain microvascular endothelial cells in vitro, demonstrating a dual adhesion phenotype mediated by distinct P. falciparum erythrocyte membrane protein 1 domains. Infect Immun 2014; 82: 949-959. https://doi.org/10.1128/IAI.01233-13
  8. Tilley L, Sougrat R, Lithgow T, Hanssen E. The twists and turns of Maurer's cleft trafficking in P. falciparum-infected erythrocytes. Traffic 2008; 9: 187-197.
  9. Mueller I, Shakri AR, Chitnis CE. Development of vaccines for Plasmodium vivax malaria. Vaccine 2015; 33: 7489-7495. https://doi.org/10.1016/j.vaccine.2015.09.060
  10. Matsumoto Y, Aikawa M, Barnwell JW. Immunoelectron microscopic localization of vivax malaria antigens to the clefts and caveola-vesicle complexes of infected erythrocytes. Am J Trop Med Hyg 1988; 39: 317-322. https://doi.org/10.4269/ajtmh.1988.39.317
  11. Aikawa M, Miller LH, Rabbege J. Caveola--vesicle complexes in the plasmalemma of erythrocytes infected by Plasmodium vivax and P. cynomolgi. Unique structures related to Schuffner's dots. Am J Pathol 1975; 79: 285-300.
  12. Udagama PV, Atkinson CT, Peiris JS, David PH, Mendis KN, Aikawa M. Immunoelectron microscopy of Schuffner's dots in Plasmodium vivax-infected human erythrocytes. Am J Pathol 1988; 131: 48-52.
  13. Barnwell JW, Ingravallo P, Galinski MR, Matsumoto Y, Aikawa M. Plasmodium vivax: malarial proteins associated with the membrane-bound caveola-vesicle complexes and cytoplasmic cleft structures of infected erythrocytes. Exp Parasitol 1990; 70: 85-99. https://doi.org/10.1016/0014-4894(90)90088-T
  14. Akinyi S, Hanssen E, Meyer EV, Jiang J, Korir CC, Singh B, Lapp S, Barnwell JW, Tilley L, Galinski MR. A 95 kDa protein of Plasmodium vivax and P. cynomolgi visualized by three-dimensional tomography in the caveola-vesicle complexes (Schuffner's dots) of infected erythrocytes is a member of the PHIST family. Mol Microbiol 2012; 84: 816-831. https://doi.org/10.1111/j.1365-2958.2012.08060.x
  15. Sanchez MR, Ramirez JA, Larriva-Sahd J, Rodriguez MH, Mancilla R, Ortiz-Ortiz L. Antigenic characterization of Plasmodium vivax with monoclonal antibodies. Am J Trop Med Hyg 1994; 51: 60-67. https://doi.org/10.4269/ajtmh.1994.51.60
  16. Molloy S. Parasitology: Layers of control for Plasmodium protein export. Nat Rev Microbiol 2013; 11: 3. https://doi.org/10.1038/nrmicro2947
  17. Sargeant TJ, Marti M, Caler E, Carlton JM, Simpson K, Speed TP, Cowman AF. Lineage-specific expansion of proteins exported to erythrocytes in malaria parasites. Genome Biol 2006; 7: R12. https://doi.org/10.1186/gb-2006-7-2-r12
  18. Lu F, Li J, Wang B, Cheng Y, Kong DH, Cui L, Ha KS, Sattabongkot J, Tsuboi T, Han ET. Profiling the humoral immune responses to Plasmodium vivax infection and identification of candidate immunogenic rhoptry-associated membrane antigen (RAMA). J Proteomics 2014; 102: 66-82. https://doi.org/10.1016/j.jprot.2014.02.029
  19. Wang B, Lu F, Cheng Y, Chen JH, Jeon HY, Ha KS, Cao J, Nyunt MH, Han JH, Lee SK, Kyaw MP, Sattabongkot J, Takashima E, Tsuboi T, Han ET. Immunoprofiling of the tryptophan-rich antigen family in Plasmodium vivax. Infect Immun 2015; 83: 3083-3095. https://doi.org/10.1128/IAI.03067-14
  20. Chen JH, Jung JW, Wang Y, Ha KS, Lu F, Lim CS, Takeo S, Tsuboi T, Han ET. Immunoproteomics profiling of blood stage Plasmodium vivax infection by high-throughput screening assays. J Proteome Res 2010; 9: 6479-6489. https://doi.org/10.1021/pr100705g
  21. Kelley LA, Mezulis S, Yates CM, Wass MN, Sternberg MJ. The Phyre2 web portal for protein modeling, prediction and analysis. Nat Protoc 2015; 10: 845-858. https://doi.org/10.1038/nprot.2015.053
  22. Marsh K, Kinyanjui S. Immune effector mechanisms in malaria. Parasite Immunol 2006; 28: 51-60. https://doi.org/10.1111/j.1365-3024.2006.00808.x
  23. Tran TM, Samal B, Kirkness E, Crompton PD. Systems immunology of human malaria. Trends Parasitol 2012; 28: 248-257. https://doi.org/10.1016/j.pt.2012.03.006
  24. Crompton PD, Moebius J, Portugal S, Waisberg M, Hart G, Garver LS, Miller LH, Barillas-Mury C, Pierce SK. Malaria immunity in man and mosquito: insights into unsolved mysteries of a deadly infectious disease. Annu Rev Immunol 2014; 32: 157-187. https://doi.org/10.1146/annurev-immunol-032713-120220
  25. Riley EM, Stewart VA. Immune mechanisms in malaria: new insights in vaccine development. Nat Med 2013; 19: 168-178. https://doi.org/10.1038/nm.3083

Cited by

  1. Molecular characterization of Plasmodium falciparum PHISTb proteins as potential targets of naturally-acquired immunity against malaria vol.5, pp.None, 2016, https://doi.org/10.12688/wellcomeopenres.15919.2
  2. Familial Hyperckemia and Calf Hypertrophy Secondary to a Caveolin-3 Mutation vol.100, pp.7, 2021, https://doi.org/10.1097/phm.0000000000001604