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Optimization of Blanching Process of Cirsium setidens by Response Surface Methodology and Influence of Blanching on Antioxidant Capacity

표면 반응 분석법에 의한 곤드레 블랜칭 최적 공정 확립 및 항산화 능에 미치는 영향

  • Jo, Hyeon Seon (Chungcheongnam-do Forest Environment Research Institute) ;
  • Ha, Yoo Jin (Department of Food and Biotechnology, Joongbu University) ;
  • Kim, Yeon Tae (Chungcheongnam-do Forest Environment Research Institute) ;
  • Kang, Gil Nam (Chungcheongnam-do Forest Environment Research Institute) ;
  • Yoo, Sun Kyun (Department of Food and Biotechnology, Joongbu University)
  • Received : 2016.10.31
  • Accepted : 2016.12.28
  • Published : 2016.12.30

Abstract

The purpose of this research was the optimization of Cirsium setidens blanched at various conditions by response surface methodology. Conducted to investigate the change of Cirsium setidens DPPH radical scavenging activity, total phenolic compounds and flavonoids during the blanching processes. With two parameters such as blanching time and temperature, response surface methodology and central composite design was used to study the combined effect of blanching time (70 to 170 seconds) and blanching temperature (70 to $99^{\circ}C$). The changes of DPPH radical scavenging activity, phenolic compounds, and flavonoids on blanching process conditions such as temperature and time were evaluated. After blanching, total phenolic compound was determined from 13.00 to 35.48 mg/ml. total flavonoids was determined from 2.31 to 8.38 mg/ml. DPPH radical scavenging activity was determined from 42.10 to 67.14 %. The optimum conditions were determined to be blanching temperature of $85^{\circ}C$ and blanching time of 150 sec.

본 연구는 표면 반응 분석법을 이용하여 곤드레 데치기 최적 공정 조건을 확립하고 각각의 공정으로부터 생산된 곤드레 추출물로부터 총 페놀 화합물, 플라보노이드의 함량, DPPH 라디칼 소거능을 분석하였다. 최적 공정 확립을 위한 데치기 공정 범위로 온도는 $70^{\circ}C-99^{\circ}C$, 시간은 70 - 170 sec.로 하였다. 데치기 공정 후 총 페놀 함량의 범위는 13.00 - 35.48 mg/g 이고 최대 예측 총 페놀함량은 35.48 mg/g, 총 페놀 함량의 경향을 보면 데치기 온도에 따라 급격히 감소하다가 증가하는 것으로 보여 지므로 온도에 의해 더 많은 영향을 받는 것으로 나타난다. 플라보노이드의 함량의 범위는 2.31 - 8.38 mg/g 이고 최대 예측 플라보노이드 함량은 8.38 mg/g 이었다. 플라보노이드 함량의 경향을 보면 데치기 시간에 따라 서서히 증가하다가 급격히 감소하는 것으로 보여 지므로 시간에 의해 더 많은 영향을 받는 것으로 나타났다. DPPH 라디칼 소거능의 범위는 42.10 - 67.14%이고 최대 예측 DPPH 라디칼 소거능은 67.14%로 측정 되었다. DPPH의 경향을 보면 데치기 온도에 따라 증가하다가 급격히 감소하는 것으로 보여 지므로 온도에 의해 더 많은 영향을 받는 것으로 나타난다. 따라서 데치기 공정 최적조건은 온도 $85^{\circ}C$와 시간 150 sec. 로 결정되었다.

Keywords

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