Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Characteristics of bioethanol production using sweet sorghum juice as a medium of the seed culture

단수수 착즙액이용 배양종균의 바이오에탄올 생산 특성 연구

  • Cha, Young-Lok (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Moon, Youn-Ho (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Yu, Gyeong-Dan (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Lee, Ji-Eun (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Choi, In-Seung (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Song, Yeon-Sang (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA) ;
  • Lee, Kyeong-Bo (Bioenergy Crop Research Institute, National Institute of Crop Science, RDA)
  • 차영록 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 문윤호 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 유경단 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 이지은 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 최인성 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 송연상 (농촌진흥청 국립식량과학원 바이오에너지작물연구소) ;
  • 이경보 (농촌진흥청 국립식량과학원 바이오에너지작물연구소)
  • Received : 2016.11.15
  • Accepted : 2016.11.18
  • Published : 2016.12.30
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Abstract

Sweet sorghum [Sorghum bicolor (L)] is one of the major crops for biofuels such as sugarcane and sugar beet which raw materials rich in saccharide. Sweet sorghum juice was extracted from the stem. It's composed of fermentable sugars such as glucose, fructose and sucrose. Ethanol from the extracted sweet sorghum juice can be easily produced by yeast fermentation process. Sweet sorghum juice is consisted of not only sugars but also various nutrients like nitrogen and phosphate. For commercial production of bioethanol, seed culture is one of the important parts of fermentation, so that optimal culture medium should be selected for the reduction of processing costs. In this study, sweet sorghum juice was estimated as a culture medium for seed culture of cellulosic bioethanol. For the comparison of cultures with various substrates, it used YPD including each 5 g/L yeast extract and peptone, sweet sorghum juice and hydrolyzed Miscanthus was taken part in the culture with 2%, 5% and 10% sugar conditions. Based on media of YPD and sweet sorghum juice, cell-mass concentration was obtained maximum more than $2.5{\times}10^8CFU/mL$ after 24 h of cultivation. Consequently sweet sorghum juice is suitable for the cell culture with more than $1.0{\times}10^8CFU/mL$ after 12 h of cultivation. This can be used as a culture medium for the cellulosic bioethanol industry.

단수수[Sorghum bicolor (L)]는 당류가 풍부한 사탕수수, 사탕무와 같은 주요 바이오연료 작물 중의 하나이다. 단수수 착즙액은 단수수 줄기를 압착하여 추출하며 주요 성분는 glucose와 fructose 그리고 sucrose로 구성되어 있다. 에탄올은 효모발효공정을 통하여 단수수 착즙액으로부터 쉽게 생산할 수 있다. 단수수 착즙액은 당류 뿐만 아니라 질소나 인산과 같은 다양한 무기영양원도 함유하고 있어 종균 배지로 활용성이 높다. 상업적인 에탄올 생산 공정에서 종균배양은 중요한 발효공정 중의 하나이며 공정비용 절감을 위해 최적화된 배지가 필수적이다. 본 연구에서는 섬유질계 바이오에탄올 생산을 위한 종균의 배지로서 단수수 착즙액의 특성을 평가하였다. 단수수착즙액의 발효기질 적합성을 평가하기 위해 YPD 및 억새 당화액을 비교하여 실험하였으며, YPD배지 중 yeast extract 와 peptone은 각각 5 g/L의 농도로 사용하였다. 각각의 기질들은 당 농도가 2%, 5%, 10%가 함유되도록 제조하여 발효를 통한 균체농도를 측정하였다. 그 결과 YPD와 단수수착즙액을 배지로 사용하였을 때 발효 24시간 후에 최대 $2.5{\times}10^8CFU/mL$ 이상의 균체농도를 나타내었다. 결과적으로 단수수착즙액은 12시간 발효 후에 $1.0{\times}10^8CFU/mL$ 균체를 생산하여 섬유질계 바이오에탄올 생산을 위한 종균 배지로서 YPD를 대체하여 사용할 수 있음을 확인하였다.

Keywords

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