한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제36권4호
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  • Pages.393-401
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  • 2016
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  • 2287-5824(pISSN)
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  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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혐기소화처리액을 배지로 이용한 클로렐라 배양액 시용이 이탈리안 라이그라스의 초기생육에 미치는 영향

Effects of Chlorella Culture Solution Using As Midium of Anaerobic Digestate on Early Growth of Italian Ryegrass (Lolium multiflorum L.)

  • 투고 : 2016.09.16
  • 심사 : 2016.11.21
  • 발행 : 2016.12.31
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초록

본 연구는 가축분뇨 혐기소화처리액과 클로렐라 배양액 시용이 이탈리안 라이그라스의 발아와 초기생육에 미치는 영향을 구명하기 위하여 수행하였다. 처리는 혐기소화처리액과 이를 배지로 활용하여 배양한 클로렐라 배양액의 희석 농도를 각각 25% (v/v), 50% (v/v), 75% (v/v), 100% (v/v)로 처리하였다. 초기생육의 평가를 위한 조사항목으로 발아율, 초장, 엽수, 엽폭, 엽록소함량 (SPAD) 측정, 지상부, 지하부 생체중과 건물중, T/R율, 뿌리길이 등을 조사하였다. 발아율은 클로렐라 배양액 25% 처리구에서 무처리구(지하수) 보다 높은 발아율을 나타내었다. 지상부 생육 특성(초장, 엽장, 엽수 및 엽폭)은 모든 처리구 중에서 클로렐라 배양액 50% 처리구가 가장 높았다. 엽록소 측정값(SPAD)은 무처리구의 30.7에 비해 클로렐라 배양액 50%처리구는 58.7로 가장 높은 수치를 나타내었다. 지상부 생체중은 클로렐라 배양액 처리구가 혐기소화액 보다 높았으며 50% 처리구에서 29.2 g으로 가장 높게 나타났다. 지하부 생체중은 클로렐라 배양액 처리구에서 21~27 g로 혐기소화처리액에 비하여 높았다. T/R율은 혐기소화처리액 처리구에서 0.98~1.04 (평균 0.97)로 클로렐라배양액 처리구의 0.94~1.06 (평균 1.02) 유의한 차이를 나타나지 않았다. 클로렐라 배양액 처리구가 혐기소화처리액 처리구보다 길었다. 이상의 결과에서 이탈리안 라이그라스의 지상부 및 뿌리 생육에 클로렐라 배양액의 초기 생육촉진 효과를 나타내었으며, 클로렐라 배양액의 적정 시용농도는 50%로 보여진다.

Anaerobic digestion is a collection of naturally occurring processes that convert organic matter and liquid residue, so-called digestate. The use of digestate biofertilizers is one of the important components of integrated nutrient management, as they are renewable sources of plant nutrients for sustainable agriculture. Seeds of Italian ryegrass (Lolium multiflorum L.) were germinated in different concentration of Chlorella in order to investigate it's the effect of Chlorella on growth parameters, seed germination and early growth. The experiment using plug tray was conducted at the green house placed in the Sangji University. The experiment consisted of nine treatments including different concentrations of Chlorella sp. culture solution and non-treated control. The germination percentage at the treatment with 25% Chlorella sp. culture solution was greater than that of control. The 50% concentration of Chlorella sp. culture solution was found to promote a better seedling growth in terms of shoot length, fresh weight and dry weight compared to the anaerobic digestate. Results showed that the best concentration of Chlorella culture solution was achieved by the 50% concentration of Chlorella culture solution treatment. As a conclusion, the application of Chlorella culture solution was found to be able to promote the germination and shoots growth of Italian ryegrass.

키워드

참고문헌

  1. Agnew, J., Lague, C., Landry, H. and Roberge, M. 2003. Handling and land application systems for solid and semi-solid manure. The Focus on the Future Conference March 25-26.
  2. Almansouri, M, Kinet, J. M, Lutts S. 2001. Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant and Soil, 231:243. https://doi.org/10.1023/A:1010378409663
  3. Bai, S.C. and Cha, Y.T. 1997. Comparison of growth and body composition in olive flounder larvae (Paralichthys olivaceus) fed domestic experimental and imported commercial microparticulated diets. Journal of Aquaculture 10:87-95.
  4. Becker, E.W. 1994. Microalgae-biotechnology and microbiology, Cambridge studies in biotechnology Vol. 10. Cambridge University Press, Cambridge, UK. pp 202.
  5. Curry, N. and P. Pillay. 2012. Biogas prediction and design of a food waste to energy system for the urban environment. Renewable Energy. 41. pp. 200-209. https://doi.org/10.1016/j.renene.2011.10.019
  6. Choi, K.C., Na, S.P., Jung, M.W., Lim, Y.C., Park, H.S., Kim, J.K., Kim, W.H., Kim, M.J., Choi, G.J., Kim, M.H., Lee, S.R., Kim, D.H. and Yook, W.B. 2012. Effect of application of cattle slurry on dry matter yield and nutritive value of whole crop barley and italian ryegrass and environmental pollution in paddy land. The Korean Society for Livestock Housing and Environment. 18: 123-130.
  7. Ha, Y.S. and Park, K.K. 2012. Cost analysis of wrap silage production in the paddy field for forage crop cropping system. Journal of the Korean Society of Grassland and Forage Science. 32:75-84. https://doi.org/10.5333/KGFS.2012.32.1.75
  8. Heo, J.Y., Shin, H.J., Oh, D.H., Cho, S.K., Yang, C.J., Kong, I.K., Lee, S.S., Choi, K.S., Choi, S.H., Kim, S.C., Choi, H.Y. and Bae, I.H. 2006. Quality properties of appenzeller cheese added with Chlorella. Korean Journal Food Science. Animal. Resources. 26:525-531.
  9. Ji, H.C., Kwon, O.D., Kim, W.H., Lim, Y.C., Cho, J.H. and Lee, K.W. 2011. Selection of pasture species at paddy field in southern region of korea. Journal of the Korean Society of Grassland and Forage Science. 31:113-118. https://doi.org/10.5333/KGFS.2011.31.2.113
  10. Kim, S.S., Park, M.K., Oh, N.S., Kim, D.C., Han, M.S. and In, M.J. 2003. Studies on quality characteristics and shelf-life of chlorella soybean (Tofu). Joural of the Korean Society Agricultural Chemistry and Biotechnology. 46: 12-15.
  11. Kim, W.H., Kim, K.Y., Jung, M.W., Ji, H.J., Lim, Y.C., Seo, S., Kim, J.D., Yoon, B.K. and Lee, H.W. 2011. Dry matter yield and forage quality at mixture of annual legumes and italian ryegrass on paddy field. Journal of the Korean Society of Grassland and Forage Science. 31:33-38. https://doi.org/10.5333/KGFS.2011.31.1.33
  12. Kim, MJ., Shim, CK., Kim, YK., Park, JH., Hong, SJ., Han, EJ. and Yoon, JC. 2014. Effect of Chlorella vulgaris on enhancement of storage and freshness in organic strawberry fruit and leaf vegetables. Korean Journal of Horticultural Science & Technology. 32:872-878. https://doi.org/10.7235/hort.2014.14107
  13. Moore, J.A. and Gamroth, M.J. 1993. Calculating the fertilizer of manure from livestock. Water Quality / Waste Management. Oregon State University.
  14. Park, H.S., Kim, J.H., Seo, S., Jung, J.S., Lee, S.H., Lee, K.W. and Choi, G.J. 2013. Effect of conditioning methods and tedding frequency on the drying rate and quality in italian ryegrass hay. Journal of the Korean Society of Grassland and Forage Science. 19(1):69-74.
  15. Park, J.G., Kim, J.D. and Kwon, C.H. 2006. Effect of liquid manure source, application rate and time. Journal of the Korean Society of Grassland and Forage Science. 26(4):227-232. https://doi.org/10.5333/KGFS.2006.26.4.227
  16. Park, M.K., Lee, J.M., Park, C.H. and In, M.J. 2002. Quality characteristics of Sulgidduk containing Chlorella powder. Journal. Korean Society Food Science. Nutrition. 31:225-229. https://doi.org/10.3746/jkfn.2002.31.2.225
  17. Raposo, R.M., Filomena, M., Morais, C.D. and Santos, R.M. 2011. Chlorella vulgaris as soil amendment: Influence of encapsulation and enrichment with rhizobacteria. International Journal Agricultural and Biological Engineering. 13:719-724.
  18. Sa, D.M. 2012. Biofertilizer development using indigenous microorganisms for healthy seedling production. Report of Korea Institute of Plannung and Evaluation for Technology in Food and Agriculture. 20-52.
  19. RDA (Rural Development Administration). 2013. Investigation guidelines for agricultural experiment. pp. 313-500.
  20. RDA (Rural Development Administration). 2013. Method of physiochemical examination by fertilizer, 144-234.
  21. Schmitt, M.A., Russelle, M.P., Randall, G.W. and Lory, J.A. 1999. Manure nitrogen crediting and management in the United States; survey of Unversity faculty. Journal of Production and Agriculture. 12:419-422. https://doi.org/10.2134/jpa1999.0419
  22. So, Y.M. 2007. Effect of Effective Microorganisms (EM) on growth properties of Brassica campestris L. M.S. thesis. Kangwon national University. Kangwon, Korea.
  23. Stirk, W.A., Balint, P., Tarkowska, D., Novak, O., Maroti, G., Ljung, K., Tureckova, V., Strnad, M., Ordog, V. and van Staden, J. 2014. Effect of light on growth and endogenous hormones in Chlorella minutissima (Trebouxiophyceae). Plant Physiol. Biochemistry. 79:66-76. https://doi.org/10.1016/j.plaphy.2014.03.005
  24. Travieso, L., Pellon, A., Benitez, F., Sanchez, E., Borja, R., O'Farrill, N. and Weiland, P. 2002. Bioalga reactor: preliminary studies for heavy metals removal. Biochemical. Engineering. Journal. 12:87-91. https://doi.org/10.1016/S1369-703X(02)00045-1