The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Management of Powdery Mildew in Squash by Plant and Alga Extract Biopesticides

  • Zhang, Shouan (Tropical Research and Education Center, University of Florida's Institute of Food and Agricultural Sciences (UF/IFAS)) ;
  • Mersha, Zelalem (Tropical Research and Education Center, University of Florida's Institute of Food and Agricultural Sciences (UF/IFAS)) ;
  • Vallad, Gary E. (Gulf Coast Research and Education Center, University of Florida's Institute of Food and Agricultural Sciences (UF/IFAS)) ;
  • Huang, Cheng-Hua (Gulf Coast Research and Education Center, University of Florida's Institute of Food and Agricultural Sciences (UF/IFAS))
  • Received : 2016.05.31
  • Accepted : 2016.08.15
  • Published : 2016.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Although many fungicides are registered for use to control powdery mildew on cucurbits, management of resistance to fungicides in pathogen populations still remains a major challenge. Two biopesticides Regalia SC and HMO 736 were evaluated in the greenhouse and field for their efficacy against powdery mildew in squash. In greenhouses, Regalia SC alone significantly (P < 0.05) reduced powdery mildew compared to the nontreated control, and was as effective as the chemical standard Procure 480SC (triflumizole). In alternation with Procure 480SC, Regalia SC demonstrated greater or equivalent effects on reducing the disease. HMO 736 alone showed varying levels of disease control, but alternating with Procure 480SC significantly improved control efficacy. In addition, application of Regalia SC or HMO 736 each in alternation with Procure 480SC significantly increased the chlorophyll content in leaves and the total fresh weight of squash plants, when compared with the water control, Regalia SC and HMO 736 alone. In field trials, application of Regalia SC and HMO 736 each alone significantly reduced disease severity in one of two field trials during the early stage of disease development, but not during later stages when disease pressure became high. Both Regalia SC and HMO 736 each applied in alternation with Procure 480SC significantly improved the control efficacy compared to Procure 480SC alone. Results from this study demonstrated that an integrated management program can be developed for powdery mildew in squash by integrating the biopesticides Regalia SC, HMO 736 with the chemical fungicide Procure 480SC.

Keywords

References

  1. Aziz, A., Poinssot, B., Daire, X., Adrian, M., Bezier, A., Lambert, B., Joubert, J. M. and Pugin, A. 2003. Laminarin elicits defense responses in grapevine and induces protection against Botrytis cinerea and Plasmopara viticola. Mol. Plant-Microbe Interact. 16:1118-1128. https://doi.org/10.1094/MPMI.2003.16.12.1118
  2. Cardinale, F., Jonak, C., Ligterink, W., Niehaus, K., Boller, T. and Hirt, H. 2000. Differential activation of four specific MAPK pathways by distinct elicitors. J. Biol. Chem. 275:36734-36740. https://doi.org/10.1074/jbc.M007418200
  3. Daayf, F., Schmitt, A. and Belanger, R. R. 1995. The effects of plant extracts of Reynoutria sachalinensis on powdery mildew development and leaf physiology of long English cucumber. Plant Dis. 79:577-580. https://doi.org/10.1094/PD-79-0577
  4. Daayf, F., Schmitt, A. and Belanger, R. R. 1997. Evidence of phytoalexins in cucumber leaves infected with powdery mildew following treatment with leaf extracts of Reynoutria sachalinensis. Plant Physiol. 113:719-727. https://doi.org/10.1104/pp.113.3.719
  5. Dik, A. and Albajes, R. 1999. Principles of epidemiology, population biology, damage relationships and integrated control of diseases and pests. In: Integrated pest and disease management in greenhouse crops, eds. by R. Albajes, M. L. Gullino, J. C. van Lenteren and Y. Elad, pp. 69-81. Kluwer Academic Publishers, Dordrecht, the Netherlands.
  6. Dik, A. J. and van der Staay, M. 1995. The effect of Milsana on cucumber powdery mildew under Dutch conditions. Int. S. Crop 59:1027-1034.
  7. Gay, J. L., Martin, M. and Ball, E. 1985. The impermeability of powdery mildew conidia and their germination in arid environments. Plant Pathol. 34:353-362. https://doi.org/10.1111/j.1365-3059.1985.tb01373.x
  8. Herger, G., Klingauf, F., Mangold, D., Pommer, E. H. and Scherer, M. 1988. Efficacy of extracts of Reynoutria sachalinensis (F. Schmidt) Nakai (Polygonaceae), against fungal diseases, especially powdery mildews. Nachr.bl. Dtsch. Pflanzenschutzd. 40:56-60.
  9. Horsfall, J. G. and Barratt, R. W. 1945. An improved grading system for measuring plant diseases. Phytopathology 35:655.
  10. Inui, H., Yamaguchi, Y. and Hirano, S. 1997. Elicitor actions of N-acetylchitooligosaccharides and laminarioligosaccharides for chitinase and L-phenylalanine ammonia-lyase induction in rice suspension culture. Biosci. Biotechnol. Biochem. 61:975-978. https://doi.org/10.1271/bbb.61.975
  11. Klarzynski, O., Plesse, B., Joubert, J. M., Yvin, J. C., Kopp, M., Kloareg, B. and Fritig, B. 2000. Linear beta-1, 3 glucans are elicitors of defense responses in tobacco. Plant Physiol. 124:1027-1038. https://doi.org/10.1104/pp.124.3.1027
  12. Konstantinidou-Doltsinis, S., Markellou, E., Kasselaki, A. M., Fanouraki, M. N., Koumaki, C. M., Schmitt, A., Liopa-Tsakalidis, A. and Malathrakis, N. E. 2006. Efficacy of $Milsana^{(R)}$, a formulated plant extract from Reynoutria sachalinensis, against powdery mildew of tomato (Leveillula taurica). BioControl 51:375-392. https://doi.org/10.1007/s10526-005-5247-1
  13. Konstantinidou-Doltsinis, S. and Schmitt, A. 1998. Impact of treatment with plant extracts from Reynoutria sachalinensis (F. Schmidt) Nakai on intensity of powdery mildew severity and yield in cucumber under high disease pressure. Crop Prot. 17:649-656. https://doi.org/10.1016/S0261-2194(98)00066-0
  14. Matheron, M. E. and Porchas, M. 2013. Efficacy of fungicides and rotational programs for management of powdery mildew von cantaloupe. Plant Dis. 97:196-200. https://doi.org/10.1094/PDIS-04-12-0370-RE
  15. McGrath, M. T. 1996a. Increased resistance to triadimefon and to benomyl in Sphaerotheca fuliginea populations following fungicide use over one season. Plant Dis. 80:633-639. https://doi.org/10.1094/PD-80-0633
  16. McGrath, M. T. 1996b. Successful management of powdery mildew in pumpkin with disease threshold-based fungicide programs. Plant Dis. 80:910-916. https://doi.org/10.1094/PD-80-0910
  17. McGrath, M. T. 2001. Fungicide resistance in cucurbit powdery mildew: experiences and challenges. Plant Dis. 85:236-245. https://doi.org/10.1094/PDIS.2001.85.3.236
  18. McGrath, M. T. and Shishkoff, N. 1999. Evaluation of biocompatible products for managing cucurbit powdery mildew. Crop Prot. 18:471-478. https://doi.org/10.1016/S0261-2194(99)00048-4
  19. McGrath, M. T., Staniszewska, H., Shishkoff, N. and Casella, G. 1996. Fungicide sensitivity of Sphaerotheca fuliginea populations in the United States. Plant Dis. 80:697-703. https://doi.org/10.1094/PD-80-0697
  20. McGrath, M. T. and Thomas, C. E. 1996. Powdery mildew. In: Compendium of cucurbit diseases, eds. by T. A. Zitter, D. L. Hopkins and C. E. Thomas, pp. 28-30. American Phytopathological Society, St. Paul, MN, USA.
  21. Menard, R., Alban, S., de Ruffray, P., Jamois, F., Franz, G., Fritig, B., Yvin, J. C. and Kauffmann, S. 2004. ${\beta}$-1,3 glucan sulfate, but not ${\beta}$-1,3 glucan, induces the salicylic acid signaling pathway in tobacco and Arabidopsis. Plant Cell 16:3020-3032. https://doi.org/10.1105/tpc.104.024968
  22. Mossler, M. A. and Nesheim, O. N. (2001 onwards). Florida crop/pest management profile: squash. University of Florida, IFAS Extension, #CIR1265. URL http://edis.ifas.ufl.edu/pi046 [25 May 2016].
  23. Nunez-Palenius, H. G., Hopkins, D. and Cantliffe, D. J. (2009 onwards). Powdery mildew of cucurbits in Florida. University of Florida, IFAS Extension, #HS1067. URL http://www.hos.ufl.edu/ProtectedAg/pdf/HS32100.pdf [25 May 2016].
  24. O'Brien, R. G., Vawdrey, L. L. and Glass, R. J. 1988. Fungicide resistance in cucurbit powdery mildew (Sphaerotheca fuliginea) and its effect on field control. Aust. J. Exp. Agric. 28:417-423. https://doi.org/10.1071/EA9880417
  25. Olson, S. M., Simonne, E. H., Stall, W. M., Roberts, P. D., Webb, S. E. and Smith, S. A. 2010. Cucurbit production in Florida. In: Vegetable production handbook for Florida 2009-2010, eds. by S. M. Olson and B. Santos, pp. 83-105. University of Florida, IFAS Extension, Gainesville, FL, USA.
  26. Petsikos-Panayotarou, N., Schmitt, A., Markellou, E., Kalamarakis, A. E., Tzempelikou, K., Siranidou, E. and Konstantinidou-Doltsinis, S. 2002. Management of cucumber powdery mildew by new formulations of Reynoutria sachalinensis (F. Schmidt) Nakai extract. Z. Pflanzenk. Pflanzen. 109:478-490.
  27. Read, S. M., Currie, G. and Bacic, A. 1996. Analysis of the structural heterogeneity of laminarin by electrospray-ionisation-mass spectrometry. Carbohydr. Res. 281:187-201. https://doi.org/10.1016/0008-6215(95)00350-9
  28. Seal, D., Zhang, S., Ozores-Hampton, M., Dittmar, P., Li, Y., Klassen, W., Wang, Q. and Olczyk, T. (2006 onwards). Summer squash production in Miami-Dade County, Florida. University of Florida, IFAS Extension, #HS-861. URL http://edis.ifas.ufl.edu/tr012 [25 May 2016].
  29. Wurms, K., Labbe, C., Benhamou, N. and Belanger, R. R. 1999. Effects of Milsana and benzothiadiazole on the ultrastructure of powdery mildew haustoria on cucumber. Phytopathology 89:728-736. https://doi.org/10.1094/PHYTO.1999.89.9.728
  30. Zhang, S. 2010. Field evaluation of biofungicides for control of powdery mildew on summer squash in Florida, winter 2009-spring 2010. Plant Dis. Manag. Rep. 4:V112.
  31. Zhang, S., Vallad, G. E., White, T. L. and Huang, C. H. 2011. Evaluation of microbial products for management of powdery mildew on summer squash and cantaloupe in Florida. Plant Dis. 95:461-468. https://doi.org/10.1094/PDIS-07-10-0521

Cited by

  1. Effect of certain plant extracts and fungicides against powdery mildew disease of Grapevines in Upper Egypt vol.50, pp.19-20, 2017, https://doi.org/10.1080/03235408.2017.1407471