Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Inhibitory effect of Rhus verniciflua Stokes extract in MCF-7 human breast cancer cells

참옻 추출물의 MCF-7 인체 유방암 세포에서 증식 억제효과

  • Kim, Min Sung (Pusan National University School of Korean Medicine) ;
  • An, Won Gun (Pusan National University School of Korean Medicine) ;
  • Lee, Jang Cheon (Pusan National University School of Korean Medicine)
  • 김민성 (부산대학교 한의학전문대학원) ;
  • 안원근 (부산대학교 한의학전문대학원) ;
  • 이장천 (부산대학교 한의학전문대학원)
  • Received : 2016.11.03
  • Accepted : 2016.11.17
  • Published : 2016.11.30
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Abstract

Objectives : The purpose of this study was to investigate the anti-cancer effects of extract of Rhus verniciflua Stokes (RVS) in human breast cancer cell lines. Methods : In cultured human breast cancer MCF-7 cells, we investigated growth inhibitory effect of RVS. MCF-7 cells were cultured with various concentrations (0, 200, 300, and 400 ug/ml) of RVS at $37^{\circ}C$ for 24 h. We performed CCK-8 assay and flow cytometry for detection of Annexin V-PI staining. Results : As a result, RVS inhibits the cell growth and induction of apoptosis in dose dependent manner in MCF-7 breast cancer cells. Conclusion : RVS has anti-cancer activities and induced apoptosis in human breast cancer MCF-7 cells. Therefore we suggest that RVS can use as a novel class of anti-cancer drugs.

Keywords

References

  1. Farber E, Rubin H. Cellular adaptation in the origin and development of cancer. Cancer Res. 1991;51(11):2751-61.
  2. Jung KW, Won YJ, Kong HJ, Oh CM, Cho H, Lee DH, Lee KH. Cancer Statistics in Korea:Incidence, Mortality, Survival, and Prevalence in 2012. Cancer Res Treat. 2015;47(2):127-41. https://doi.org/10.4143/crt.2015.060
  3. Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: from ancient medicine to current clinical trials. Cell and Mol Life Sci 2008;65(11):1631-52. https://doi.org/10.1007/s00018-008-7452-4
  4. Namba T. Coloured illustrations of Wakan-Yaku. Hoikusha Publishing Co.1980: p215.
  5. Jeon WK, Lee JH, Kim HK, Lee AY, Lee SO, Kim YS, Ryu SY, Kim SY, Lee YJ, Ko BS. Anti-platelet effects of bioactive compounds isolated from the bark of Rhus verniciflua Stokes. J Ethnopharmacol. 2006;106(1):62-9. https://doi.org/10.1016/j.jep.2005.12.015
  6. Kim J, Kim KL. Study on the Characteristics of Fermented Rhus verniciflua Stem Bark (FRVSB) as a Cosmetic Raw Material. 2015;13(5):559-67.
  7. Son YO, Lee KY, Lee JC, Jang HS, Kim JG, Jeon YM, Jang YS. Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. Toxicol Lett. 2005;155(1):115-25. https://doi.org/10.1016/j.toxlet.2004.09.003
  8. Park, KY, Jung GO, Lee KT, Choi JW, Choi MY, Kim GT, Park HJ. Antimutagenic acrivity of flavonoids from the heartwood of verniciflua. J. Ethnopharmacol. 2004;90(1): 73-9. https://doi.org/10.1016/j.jep.2003.09.043
  9. Lee JS, Yoo DY. Effect of water extract of Kun Chil San on human female tumor cells. 1997;10(2):201-21
  10. Hong SH, Han MH, Choi YH, Park SE. Induction of p53-Dependent G1 Cell Cycle Arrest by Rhus verniciflua. Stokes Extract in Human Breast Carcinoma MCF-7 Cells. J.Int. Korea Med. 2015;36(1):13-21
  11. An JY, Ko SG, Ko H. Effects of Rhus verniciflua Stokes Extract on Cell Viability, Cell Cycle Progression and Apoptosis of AGS Cell J Physiol&Pathol Korean Med. 2007;20(3):701-9.
  12. Choi HS, Kim MK, Choi YK, Shin YC, Cho SG, Ko SG. Rhus verniciflua Stokes (RVS) and butein induce apoptosis of paclitaxel-resistant SKOV-3/PAX ovarian cancer cells through inhibition of AKT phosphorylation. BMC Complement Altern Med. 2016;16:122. https://doi.org/10.1186/s12906-016-1103-3
  13. Lee JC, Lee KY, Kim J, Na CS, Jung NC, Chung GH, Jang YS. Extract from Rhus verniciflua Stokes is capable of inhibiting the growth of human lymphoma cells. Food Chem Toxicol. 2004;42(9):1383-88. https://doi.org/10.1016/j.fct.2004.03.012
  14. Lee SH, Jeong HS, Kang TS. Antimicrobial and antioxidative activities of hot water extracts from heat-air drid Rhus verniciflua stokes. Food Eng. Prog. 2013;17(1):1-7.
  15. Lee JO, Moon JW, Lee SK, Kim SM, Kim N, Ko SG, Kim HS, Park SH. Rhus verniciflua extract modulates survival of MCF-7 breast cancer cells through the modulation of AMPK-pathway. Biol Pharm Bull. 2014;37(5):794-801. https://doi.org/10.1248/bpb.b13-00893
  16. Park HS. Antioxidant and nitrite scavenging activities of solvent extracts from Rhus verniciflua Stokes. J. East Asian Soc. Dietary Life. 2011;21(5):677-682.
  17. Kim JS, Kwon YS, Chun WJ, Kim TY, Sun J, Yu CY, Kim MJ. Rhus verniciflua Stokes flavonoid extracts have antioxidant, antimicrobial and a-glucosidase inhibitory effect. Food Chem. 2010;120(2):539-543. https://doi.org/10.1016/j.foodchem.2009.10.051
  18. Huang Z. Bcl-2 family proteins as targets for anticancer drug design. Oncogene. 2000;19(56):6627-6631. https://doi.org/10.1038/sj.onc.1204087
  19. Dorssers LC, Van der Flier S, Brinkman A, van Agthoven T, Veldscholte J, Berns EM, Klijn JG, Beex LV, Foekens JA. Tamoxifen resistance in breast cancer: elucidating mechanisms. Drugs. 2001;61(12):1721-1733. https://doi.org/10.2165/00003495-200161120-00004